|本期目录/Table of Contents|

[1]解修超,贾娇,宋玉,等.响应面法优化黑木耳新品种森盛1号液体发酵条件[J].江苏农业科学,2022,50(14):138-143.
 Xie Xiuchao,et al.Optimization of liquid fermentation conditions for a new black fungus variety Sensheng No.1 by response surface methodology[J].Jiangsu Agricultural Sciences,2022,50(14):138-143.
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响应面法优化黑木耳新品种森盛1号液体发酵条件(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第14期
页码:
138-143
栏目:
园艺与林学
出版日期:
2022-07-20

文章信息/Info

Title:
Optimization of liquid fermentation conditions for a new black fungus variety Sensheng No.1 by response surface methodology
作者:
解修超12贾娇12宋玉12谢海彬23邓百万12孙娅1徐易洁1
1.陕西理工大学生物科学与工程学院,陕西汉中 723000; 2.陕西省食药用菌工程技术研究中心,陕西汉中 723000;3.汉中植物研究所,陕西汉中 723000
Author(s):
Xie Xiuchaoet al
关键词:
黑木耳响应面工艺优化Plackett-BurmanBox-Behnken
Keywords:
-
分类号:
S646.604
DOI:
-
文献标志码:
A
摘要:
为缩短黑木耳新品种森盛1号的液体种生产周期,提高菌丝体产量,拟为黑木耳液体种应用于生产提供技术参考,通过Plackett-Burman设计试验,筛选到葡萄糖含量、磷酸二氢钾含量、初始pH值为影响菌丝生物量的3个显著性因子,通过最陡爬坡试验得到其最佳相应区域,最后应用响应面Box-Behnken分析法和验证试验对森盛1号黑木耳菌株的液体发酵工艺进行优化。结果表明,森盛1号的最佳发酵工艺条件为马铃薯200.0 g/L、葡萄糖50.27 g/L、蛋白胨4.5 g/L、麦麸15.0 g/L、磷酸二氢钾4.93 g/L、七水硫酸镁1.0 g/L、初始pH值 5.1、接种量4%、温度28.0 ℃、160 r/min摇床发酵8 d,菌丝干质量可达到21.57 g/L,比优化前提高了23.53%,误差仅为1.75%。该模型能准确地预测出菌丝体产量变化情况,证明响应面法优化黑木耳新品种森盛1号是有效可行的。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2021-09-10
基金项目:陕西省重点研发计划(编号:2020NY-062);陕西省科技创新基地建设项目(编号:2021ZY2-JD-09)。
作者简介:解修超(1978—),男,山东临沂人,博士,副教授,主要从事微生物资源开发与利用研究。E-mail:xiexiuchao@126.com。
通信作者:谢海彬,副研究员,主要从事食用菌品种选育及栽培技术研究。E-mail:770335223@qq.com。
更新日期/Last Update: 2022-07-20