|本期目录/Table of Contents|

[1]胡家达,吴海智,薛昌安,等.胆固醇和磷脂酰胆碱对山羊精液冷冻保存效果的影响[J].江苏农业科学,2022,50(14):159-165.
　Hu Jiada,et al.Influence of cholesterol and phosphatidylcholine on cryopreservation of goat semen[J].Jiangsu Agricultural Sciences,2022,50(14):159-165.
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胆固醇和磷脂酰胆碱对山羊精液冷冻保存效果的影响(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第14期
页码:
159-165
栏目:
畜牧兽医与水产蚕桑
出版日期:
2022-07-20

文章信息/Info

Title:
Influence of cholesterol and phosphatidylcholine on cryopreservation of goat semen
作者:
胡家达1; 吴海智2; 薛昌安1; 顾紫薇1; 王若茜1; 潘江山1; 高永恒1; 刘亚1; 方富贵1; 张运海1; 丁建平1; 李运生1
1.安徽农业大学动物科技学院，安徽合肥 230036； 2.安徽省畜牧技术推广总站，安徽合肥 230001
Author(s):
Hu Jiada; et al
关键词:
山羊; 精液; 冷冻保存; 胆固醇; 磷脂酰胆碱
Keywords:
-
分类号:
S827.3+4
DOI:
-
文献标志码:
A
摘要:
为探究胆固醇(CHL)和磷脂酰胆碱(PC)对山羊精液冷冻保存效果，分别在精液冷冻稀释液中添加不同浓度的CHL(1.0、2.5、5.0、10.0 g/L)、PC(10、15、20、25 g/L)，以15%卵黄(EY)作为对照。通过测定冷冻解冻后精子的活力、顶体完整率、精子运动参数、质膜完整率、精子磷脂和胆固醇含量来判断保存效果。结果表明，精液冷冻显著降低山羊精子磷脂和胆固醇含量，分别添加5 g/L CHL 和10 g/L PC，精子冷冻解冻后，在活力、顶体完整率、质膜完整率和运动参数与对照组差异不显著。进一步将CHL和PC混合添加发现，4 g/L CHL+16 g/L PC解冻后精子活力、曲线速率、平均路径速度和顶体完整率均显著高于对照组(P<0.05)，且可以改善冷冻后精子磷脂和胆固醇含量。运用 4 g/L CHL+16 g/L PC冷冻保存精子进行山羊人工授精，其受胎率和产仔率与EY组差异不显著，冷冻稀释液中添加4 g/L CHL+16 g/L PC显著提高山羊精液冷冻保存效果。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2021-09-09
基金项目：安徽省中央引导地方科技发展专项(编号：202007d06020005)；安徽省科技重大专项(编号：202103b06020003)；临泉县2021年度“肉羊产业+金融+科技” 发展试点科技项目(编号：LQRJK2021-02)；亳州市科技重大专项(编号：BZSKXJSJ2020-62)。
作者简介：胡家达(1997—)，男，河南信阳人，硕士研究生，主要从事动物生殖生理相关研究。E-mail：1778686466@qq.com。
通信作者：李运生，博士，副教授，主要从事动物生殖生理相关研究。E-mail：lys@ahau.edu.cn。
更新日期/Last Update: 2022-07-20