|本期目录/Table of Contents|

[1]张中起,梁邦平,王俊涛,等.大豆VLN基因家族的全基因组鉴定及组织表达分析[J].江苏农业科学,2023,51(3):35-45.
　Zhang Zhongqi,et al.Genome-wide identification and tissue expression analysis of VLN gene family in soybean[J].Jiangsu Agricultural Sciences,2023,51(3):35-45.
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大豆VLN基因家族的全基因组鉴定及组织表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第3期
页码:
35-45
栏目:
生物技术
出版日期:
2023-02-05

文章信息/Info

Title:
Genome-wide identification and tissue expression analysis of VLN gene family in soybean
作者:
张中起;  梁邦平;  王俊涛;  高保民;  刘艳;  王秋玲
菏泽市农业科学院大豆研究所，山东菏泽 274000
Author(s):
Zhang Zhongqi; et al
关键词:
大豆; VLN基因家族; 鉴定; 表达分析
Keywords:
-
分类号:
S565.103.2
DOI:
-
文献标志码:
A
摘要:
绒毛蛋白(villin，VLN)是最重要的肌动蛋白结合蛋白(actin-binding proteins，简称ABPs)之一，参与肌动蛋白丝的解聚和组装，具有成核、成帽、切割和捆绑等重要的功能。分析大豆VLN基因家族成员的数量、序列特征、系统进化和表达模式，为阐明大豆VLNs基因的生物学功能奠定基础。通过HMM方法检索大豆中黄13的参考基因组，鉴定大豆VLN基因家族成员；利用在线网站NCBI-CDD、SMART、PDB、ExPASy和Plant-mPLoc分析大豆VLNs蛋白的理化性质；利用MG2C、GSDS、MEME和PlantCARE分析大豆VLNs基因的序列特征；通过转录组测序数据分析大豆VLNs基因表达模式；利用在线网站STRING 11.5构建大豆VLNs蛋白互作网络。从大豆中共鉴定出8个VLNs基因，它们分布于8条染色体，亚细胞定位均位于细胞质。大豆VLN家族基因的外显子/内含子数量较多且基因序列较长，启动子区有大量与生长发育、植物激素、生物胁迫和非生物胁迫等相关的响应元件，成员间蛋白序列有一定的保守性和相似性，大豆VLN家族蛋白在系统进化上分为3组。部分大豆VLNs基因在根、茎、花、荚和种子中特异性表达，蛋白互作网络显示成员间存在潜在的相互作用。大豆VLN基因家族的全基因组鉴定及生物信息学分析结果，为进一步解析大豆VLNs基因功能提供了重要的理论基础。大豆共有8个VLNs基因，它们均定位于细胞质，具有响应生长发育、激素和胁迫的调控元件，GmVLN3、GmVLN5和GmVLN6在根、茎、荚中特异性优势表达。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2022-03-16
基金项目：山东省现代农业产业技术体系杂粮创新团队(编号：SDAIT-15-02)；山东省农业良种工程优质高蛋白大豆突破性新品种选育(编号：2019LZGC004)。
作者简介：张中起(1991—)，男，山东菏泽人，硕士，农艺师，主要从事大豆遗传育种研究。E-mail：soybean2021@163.com。
通信作者：王秋玲，研究员，主要从事大豆遗传育种研究。E-mail：wangqiuling@163.com。
更新日期/Last Update: 2023-02-05