|本期目录/Table of Contents|

[1]段明.基于Illumina MiSeq技术分析谷子根际丛枝菌根真菌群落多样性[J].江苏农业科学,2023,51(6):222-229.
　Duan Ming.Study on arbuscular mycorrhizal fungi diversity in rhizosphere of foxtail millet based on Illumina MiSeq technology[J].Jiangsu Agricultural Sciences,2023,51(6):222-229.
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基于Illumina MiSeq技术分析谷子根际丛枝菌根真菌群落多样性(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第6期
页码:
222-229
栏目:
资源与环境
出版日期:
2023-03-20

文章信息/Info

Title:
Study on arbuscular mycorrhizal fungi diversity in rhizosphere of foxtail millet based on Illumina MiSeq technology
作者:
段明
山西农业大学实验教学中心，山西太谷 030801
Author(s):
Duan Ming
关键词:
谷子; 丛枝菌根; Illumina Miseq技术; 群落多样性
Keywords:
-
分类号:
S515.01；S182
DOI:
-
文献标志码:
A
摘要:
为研究谷子(Setaria italica)根际丛枝菌根真菌(AMF)群落的多样性特征，以便充分发掘和利用AMF优势菌群。本试验对土壤进行正常浇水与干旱处理，土壤含水量分别为7.6%和3.7%，并采集谷子(豫谷、安陵和沁黄3个品种)根系及无寄主对照土壤(CK)，获得全部DNA并对其中的18S rRNA基因进行PCR扩增，并运用MiSeq高通量测序技术和生物信息学研究谷子的根际AMF及群落特征。物种多样性分析结果显示，在正常浇水与干旱处理下，谷子根系与CK的OTU数量在1 632~4 290个之间，共检测并鉴定出AMF 126种，隶属1门9属。其中，按优势种的占比高低依次是原囊霉属(Acaulospora)、球囊霉属(Glomus)、类球囊霉属(Paraglomus)和双型囊霉属(Ambispora)。与CK相比，谷子根际AMF群落多样性减少，AMF在原囊霉属水平上丰度显著降低。但正常浇水与干旱处理下，谷子根际AMF群落多样性并未发生显著变化。研究揭示了谷子根际AMF群落组成，主要为球囊霉属；与CK相比，3个谷子品种AMF群落分布在多样性及丰度方面具有普遍的相似性。此外，与正常浇水相比，干旱条件下谷子根际丛枝菌根群落多样性及丰度保持稳定，维持在较高的水平，可保证谷子根系对水分和养分的高效吸收与利用。
Abstract:
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参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2022-05-13
基金项目：山西省自然科学研究面上项目(编号：20210302123395)；山西农业大学科技创新基金(编号：2017GPY01)。
作者简介：段明(1984—)，男，山东威海人，博士，副教授，主要从事植物抗逆分子生物学研究。E-mail：duanming840305@163.com。
更新日期/Last Update: 2023-03-20