|本期目录/Table of Contents|

[1]王喜英,赵辉,卢志宏,等.汞胁迫对蔬菜土壤细菌和真菌群落结构及丰度的影响[J].江苏农业科学,2023,51(14):229-239.
　Wang Xiying,et al.Influences of Hg stress on structure and abundance of soil bacterial and fungal communities in soils for growing vegetables[J].Jiangsu Agricultural Sciences,2023,51(14):229-239.
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汞胁迫对蔬菜土壤细菌和真菌群落结构及丰度的影响(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第14期
页码:
229-239
栏目:
资源与环境
出版日期:
2023-07-20

文章信息/Info

Title:
Influences of Hg stress on structure and abundance of soil bacterial and fungal communities in soils for growing vegetables
作者:
王喜英; 赵辉; 卢志宏; 谭智勇; 宋希娟
铜仁学院，贵州铜仁 554300
Author(s):
Wang Xiying; et al
关键词:
汞; 蔬菜土壤; hgcA基因; 细菌; 真菌; 高通量测序
Keywords:
-
分类号:
S182
DOI:
-
文献标志码:
A
摘要:
汞(Hg)是一种广泛存在于土壤环境中的全球污染物之一，土壤微生物对汞胁迫的敏感性强于动植物，可从微生物角度为蔬菜土壤汞污染生态风险评估提供科学依据。采用盆栽试验，应用荧光定量PCR和高通量测序(Illumina HiSeq)技术，分析对照(CK)、低浓度汞(T1)、中浓度汞(T2)和高浓度汞(T3)胁迫处理下蔬菜土壤hgcA基因数量、细菌数量、真菌数量和群落结构变化特征。结果表明，T1处理增加细菌和hgcA基因数量，分别比CK、T2和T3提高了37.48%和 12.01%、57.31%和 19.37%、88.85%和14.82%。汞胁迫降低了真菌数量，其中T2处理降低最显著。T3处理降低了土壤细菌群落α多样性指数(丰富度和多样性)，T1处理降低了土壤真菌群落α多样性指数(丰富度和多样性)。土壤细菌门水平上，共获得18个类群，其中放线菌门、变形菌门和绿弯菌门为优势类群，且在不同处理间差异极显著。T2和T3处理分别显著增加了变形菌门和放线菌门相对丰度。绿弯菌门相对丰度均表现随汞浓度增加逐渐递减的趋势。土壤真菌门水平上，共获得9个类群，其中子囊菌门、被孢菌门和担子菌门为优势类群，其相对丰度共占真菌群落的94.64%。随着汞浓度增加，子囊菌门相对丰度递增，被孢菌门相对丰度递减。担子菌门在 T1 处理中最高(5.40%)。土壤pH值、铵态氮含量、硝态氮含量和全汞含量与土壤细菌数量、细菌和真菌群落结构有显著关系。综上，汞胁迫对蔬菜土壤hgcA基因、细菌和真菌数量有显著影响，高汞胁迫造成了土壤细菌多样性减少，细菌和真菌群落结构和组成发生变化，而细菌群落对汞胁迫的敏感性强于真菌。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2022-07-20
基金项目：贵州省教育厅自然科学项目(编号：黔教合KY字［2020］163)；铜仁学院博士科研启动基金(编号：tyxyDH2002、tyxyDH1603)；绿色农药与农业生物工程教育部重点实验室开放基金(编号：黔教合KY字［2019］036)；铜仁学院硕士点及学科建设研究子项目(编号：trxyxwdxm-027)；贵州省科技项目(编号：黔科合SY字［2014］3035)。
作者简介：王喜英(1981—)，女，河南汝南人，硕士，讲师，主要从事生物化学及生物信息学分析。E-mail：810315971@qq.com。
通信作者：赵辉，博士，教授，主要从事土壤微生态及作物栽培研究。E-mail：yancao504@163.com。
更新日期/Last Update: 2023-07-20