|本期目录/Table of Contents|

[1]李健,李曦,农艳丰.芒果bZIP转录因子基因的克隆、亚细胞定位及表达分析[J].江苏农业科学,2023,51(21):29-36.
　Li Jian,et al.Cloning，subcellular localization and expression analysis of mango bZIP transcription factor gene[J].Jiangsu Agricultural Sciences,2023,51(21):29-36.
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芒果bZIP转录因子基因的克隆、亚细胞定位及表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第21期
页码:
29-36
栏目:
生物技术
出版日期:
2023-11-05

文章信息/Info

Title:
Cloning，subcellular localization and expression analysis of mango bZIP transcription factor gene
作者:
李健; 李曦; 农艳丰
百色学院农业与食品工程学院/广西芒果生物学重点实验室/亚热带特色农业产业学院，广西百色 533000
Author(s):
Li Jian; et al
关键词:
芒果; 碱性亮氨酸拉链(bZIP)转录因子; 基因克隆; 基因表达; 胁迫处理
Keywords:
-
分类号:
S667.701
DOI:
-
文献标志码:
A
摘要:
碱性亮氨酸拉链(bZIP)转录因子在调控植物生长发育及抵抗非生物胁迫过程中具有重要作用。本研究通过RACE技术，获得了全长1 317 bp的芒果bZIP转录因子基因(MibZIP46)。系统进化树分析结果表明，MibZIP46与同样是漆树科的阿月浑子的bZIP亲缘关系最为接近，同源性最高，与甜橙的同源性次之。构建了原核表达重组质粒pCZN 1-MibZIP46，经0.2 mmol/L IPTG诱导，目的蛋白以包涵体的形式表达，经镍柱纯化，获得高纯度(90%)的目的蛋白并成功进行Western blotting鉴定；MibZIP46蛋白的亚细胞定位分析结果表明，融合的目的蛋白在烟草叶片细胞中定位于细胞核；实时荧光定量PCR分析结果表明，200 mmol/L NaCl、15% PEG和0.1 mmol/L ABA胁迫处理，均能不同程度诱导MibZIP46的表达。本研究为进一步挖掘利用芒果的抗逆相关基因提供了理论依据。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2023-03-15
基金项目：广西自然科学基金(编号：2018GXNSFBA050026)；广西高校中青年教师科研基础能力提升项目(编号：2019KY0743、2020KY19025)。
作者简介：李健(1986—)，男，广西百色人，博士，研究方向为植物抗逆生理与分子生物学。E-mail：337375159@qq.com。
通信作者：农艳丰，硕士，高级实验师，研究方向为植物生物技术。E-mail：1477078570@qq.com。
更新日期/Last Update: 2023-11-05