|本期目录/Table of Contents|

[1]吴芸,欧正淼,殴小嫚,等.赤水乌骨鸡PTGDS基因克隆、序列特征及组织表达分析[J].江苏农业科学,2023,51(23):22-27.
 Wu Yun,et al.Cloning,sequence characteristics and tissue expression of PTGDS gene in Chishui black bone chicken[J].Jiangsu Agricultural Sciences,2023,51(23):22-27.
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赤水乌骨鸡PTGDS基因克隆、序列特征及组织表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第23期
页码:
22-27
栏目:
生物技术
出版日期:
2023-12-05

文章信息/Info

Title:
Cloning,sequence characteristics and tissue expression of PTGDS gene in Chishui black bone chicken
作者:
吴芸1欧正淼2殴小嫚2陈粉粉2闫吉美1李盼盼1
1.遵义师范学院生物与农业科技学院,贵州遵义 563006; 2.西南林业大学生命科学学院,云南昆明 650224
Author(s):
Wu Yunet al
关键词:
赤水乌骨鸡PTGDS基因生物信息学分析组织mRNA表达
Keywords:
-
分类号:
S831.2
DOI:
-
文献标志码:
A
摘要:
为研究赤水乌骨鸡PTGDS基因的结构和功能,检测其在赤水乌骨鸡不同组织、胸肌和腿肌中的表达情况,探究PTGDS基因对赤水乌骨鸡肉质性能的影响。运用RT-PCR获得PTGDS基因CDS序列,对其氨基酸序列进行生物信息学分析,揭示其结构特征和亲缘关系,同时通过qPCR检测赤水乌骨鸡PTGDS基因在同一时期不同组织中的表达特性。结果显示,赤水乌骨鸡PTGDS基因编码区长558 bp,编码186个氨基酸,保守性分析发现赤水乌骨鸡PTGDS基因与原鸡的同源性最高,核酸和氨基酸序列比对同源性分别为100%;系统进化分析发现,赤水乌骨鸡与原鸡的亲缘关系最近,与黑天鹅和鸿雁的亲缘关系最远。PTGDS基因在各组织中均有表达,在肾中的表达量最高,显著高于其他组织(P<0.05);肌肉中腿肌的表达量高于胸肌,但差异不显著(P>0.05)。研究结果可为进一步揭示PTGDS基因的功能和调控机制提供参考。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2023-02-03
基金项目:贵州省教育厅特色领域项目(编号:黔教合KY字[2019]076);遵义师范学院服务地方产业革命项目(编号:遵师合地方产业字[2020]05号);遵义师范学院博士启动基金(编号:遵师BS[2017]15号)。
作者简介:吴芸(1982—),女,重庆人,博士,副教授,主要从事家禽遗传育种研究。E-mail:kyzywy002@163.com。
更新日期/Last Update: 2023-12-05