|本期目录/Table of Contents|

[1]张珍华,蒋素华,武思,等.马铃薯响应低氮肥胁迫AP2转录因子的生物信息分析[J].江苏农业科学,2024,52(4):55-62.
 Zhang Zhenhua,et al.Bioinformatics analysis of AP2 transcription factors in potato in response to low nitrogen fertilizer stress[J].Jiangsu Agricultural Sciences,2024,52(4):55-62.
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马铃薯响应低氮肥胁迫AP2转录因子的生物信息分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第4期
页码:
55-62
栏目:
生物技术
出版日期:
2024-02-20

文章信息/Info

Title:
Bioinformatics analysis of AP2 transcription factors in potato in response to low nitrogen fertilizer stress
作者:
张珍华蒋素华武思梁芳牛苏燕
郑州师范学院,河南郑州 450044
Author(s):
Zhang Zhenhuaet al
关键词:
马铃薯低氮肥胁迫AP2转录因子生物信息学分析响应
Keywords:
-
分类号:
S532.01
DOI:
-
文献标志码:
A
摘要:
为研究马铃薯(Solanum tuberosum)AP2 亚组类转录因子家族对低氮肥胁迫的响应特征,以马铃薯品种Russet Burbank为材料,分别种植于氮肥供应充足和不足条件下处理10 d后,收取不同处理下的叶片和根构建转录组测序文库,基于转录组测序结果筛选出AP2转录因子并对其进行生物信息分析和对低氮肥胁迫的响应分析。结果表明,18个马铃薯AP2转录因子不均匀地分布在11条染色体上,且都含有不同数目的保守基序结构,亲缘关系分析发现有15个马铃薯AP2家族蛋白与拟南芥的AP2蛋白聚集在了一起。基于马铃薯转录组数据,发现根中12个基因在低氮肥胁迫处理后被抑制性表达,其中3个基因显著降低;在叶片中9个基因被抑制表达,2个基因达到了显著性差异。此外,还发现AP2转录因子在马铃薯不同组织中的表达水平有很大差异,其中14个AP2转录因子在根和叶片中的表达量呈显著性差异。根据亲缘关系和功能分析,推测PGSC0003DMG400027502、PGSC0003DMG400012181和PGSC0003DMG400013587可作为候选的响应氮肥胁迫的关键转录因子,该研究为下一步研究马铃薯AP2响应低氮肥胁迫的生物学功能奠定了基础。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2023-04-21
基金项目:河南省科技攻关项目 (编号:202102110167、212102110211);郑州师范学院高层次人才科研启动专项经费 (编号:2018)。
作者简介:张珍华(1985—),男,河南林州人,硕士,主要从事生物信息学及作物育种研究。E-mail:zhenhuayuemu@163.com。
通信作者:牛苏燕,博士,讲师,主要从事薯类作物育种及功能基因组学研究。E-mail:niusuyan1234@163.com。
更新日期/Last Update: 2024-03-20