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[1]李欣,李鲁华,任明见,等.小麦TaMICU1-6A基因的克隆、生物信息学及表达分析[J].江苏农业科学,2024,52(5):42-51.
　Lixin,et al.Cloning，bioinformatics and expression analysis of TaMICU1-6A gene in wheat[J].Jiangsu Agricultural Sciences,2024,52(5):42-51.
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小麦TaMICU1-6A基因的克隆、生物信息学及表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第5期
页码:
42-51
栏目:
生物技术
出版日期:
2024-03-05

文章信息/Info

Title:
Cloning，bioinformatics and expression analysis of TaMICU1-6A gene in wheat
作者:
李欣; 李鲁华; 任明见; 安畅; 洪鼎立; 赵鹏鹏; 徐如宏
贵州大学农学院，贵州贵阳 550025；国家小麦改良中心贵阳分中心，贵州贵阳 550025
Author(s):
Lixin; et al
关键词:
小麦; TaMICU1基因; 基因克隆; 生物信息学分析; 基因表达
Keywords:
-
分类号:
Q78；S512.101
DOI:
-
文献标志码:
A
摘要:
为了探讨线粒体稳态调节蛋白TaMICU1基因在小麦生长发育中的功能，以小麦中国春为材料，对其TaMICU1-6A基因进行克隆、生物信息学分析、表达分析研究，结果表明，小麦TaMICU1-6A基因全长为1 398 bp，编码465个氨基酸，定位于线粒体，具有2个EF-hand家族的保守结构域，启动子含3种激素响应元件、3种光响应相关反应元件、1个缺氧特异性诱导相关元件；多重序列比对发现，其与野生二粒小麦、乌拉尔图小麦、小麦中的同源蛋白或同源基因的亲缘关系比较近。RT-PCR结果表明，TaMICU1-6A基因具有组织特异性，不同非生物胁迫、不同激素处理下在根、茎、叶中的表达水平不同。黑暗处理下，TaMICU1-6A基因在根、茎、叶中的表达水平均在6 h时达到最低；低温处理下，TaMICU1-6A基因在根、茎中的表达水平呈先降后升的趋势；在赤霉素处理下，TaMICU1-6A基因在叶片中的表达水平在6 h时达到最高。综上所述，推测TaMICU1-6A基因通过激素介导的信号途径参与不同的非生物胁迫。期待本研究结果可为进一步探讨小麦TaMICU1基因在逆境下的生物学功能提供一定的参考价值。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2023-05-18
基金项目：国家自然科学基金(编号：3216150357)；贵州省科技计划项目(编号：黔科合基础〔2020〕1Z018 号)；贵州省科技支撑计划项目(编号：黔科合支撑〔2021〕一般272)。
作者简介：李欣(1998—)，女，贵州毕节人，硕士研究生，研究方向为作物学遗传育种。E-mail：1196372633@qq.com。
通信作者：徐如宏，教授，主要从事小麦遗传育种研究工作。E-mail：xrhgz@163.com。
更新日期/Last Update: 2024-03-05