[1]董晶晶,薛春雨,习丙文,等.团头鲂黑素皮质素受体2基因克隆、组织分布和应激后的表达分析[J].江苏农业科学,2014,42(06):20-26.
　Dong Jingjing,et al.Gene cloning，tissue distribution and expression after stress of melanocortin receptor 2 in Megalobrama amblycephala[J].Jiangsu Agricultural Sciences,2014,42(06):20-26.
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团头鲂黑素皮质素受体2基因克隆、组织分布和应激后的表达分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年06期
页码:
20-26
栏目:
生物技术
出版日期:
2014-06-25

文章信息/Info

Title:
Gene cloning，tissue distribution and expression after stress of melanocortin receptor 2 in Megalobrama amblycephala
作者:
董晶晶1; 2;  薛春雨1; 2;  习丙文1;  梁利国2;  刘波2;  任鸣春1;  谢骏1; 2
1.南京农业大学无锡渔业学院，江苏 无锡 214081；2.中国水产科学研究院淡水渔业研究中心/农业部淡水渔业和种质资源利用重点实验室，江苏无锡 214081
Author(s):
Dong Jingjing; et al
关键词:
团头鲂; MC2R基因; 皮质醇; 克隆; 组织表达; 应激
Keywords:
-
分类号:
S917
DOI:
-
文献标志码:
A
摘要:
在神经内分泌下丘脑-垂体-肾间组织轴调控中，黑素皮质素受体2(MC2R)与垂体释放的促肾上腺皮质激素结合而激活相应的cAMP信号转导途径，对鱼类的应激反应调控具有重要的作用。本研究通过PCR、RACE技术克隆获得团头鲂MC2R mRNA序列，并通过实时定量PCR分析了团头鲂MC2R组织分布情况及捕捞胁迫后的表达变化。结果显示，获得的MC2R mRNA序列开放阅读框为915 bp，编码304个氨基酸。序列同源性及系统育分析显示，团头鲂MC2R氨基酸序列与鲤科鱼类的MC2R聚为一支，与金鱼(Carassius auratus)MC2R的氨基酸序列同源性最高(87%)。组织分布结果表明，头肾中MC2R的表达量最高，脾脏、精巢和脑中有相对较高的表达量，中肾、肝、肠和肌肉等MC2R的表达量相对较低。经捕捞出水体60 s的急性应激处理后，皮质醇在4 h时显著升高，24 h时表现出下降的趋势；MC2R基因表达量在头肾中变化不显著，然而脾、脑和精巢中MC2R 在4 h时表达量相对于1 h则呈现出显著的下降。通过对团头鲂应激后主要调控因子变化规律的研究，可为鱼类应激后调控措施的实施提供参考依据。
Abstract:
-

参考文献/References:

［1］Bonga S E W. The stress response in fish［J］. Physiological Reviews，1997，77(3)：591-625.
［2］Flik G，Klaren P H，van den Burg E H，et al. CRF and stress in fish［J］. General and Comparative Endocrinology，2006，146(1)：36-44.
［3］Alsop D，Vijayan M M. Molecular programming of the corticosteroid stress axis during zebrafish development［J］. Comparative Biochemistry and Physiology Part A：Molecular and Integrative Physiology，2009，153(1)：49-54.
［4］Chida D，Nakagawa S，Nagai S，et al. Melanocortin 2 receptor is required for adrenal gland development，steroidogenesis，and neonatal gluconeogenesis［J］. Proceedings of the National Academy of Sciences，2007，104(46)：18205-18210.
［5］Mountjoy K G，Robbins L S，Mortrud M T，et al. The cloning of a family of genes that encode the melanocortin receptors［J］. Science，1992，257(5074)：1248-1251.
［6］Kapas S，Cammas F M，Hinson J P，et al. Agonist and receptor binding properties of adrenocorticotropin peptides using the cloned mouse adrenocorticotropin receptor expressed in a stably transfected HeLa cell line［J］. Endocrinology，1996，137(8)：3291-3294.
［7］Agulleiro M J，Roy S，Sánchez E，et al. Role of melanocortin receptor accessory proteins in the function of zebrafish melanocortin receptor type 2［J］. Molecular and Cellular Endocrinology，2010，320(1/2)：145-152.
［8］Liang L，Sebag J A，Eagelston L，et al. Functional expression of frog and rainbow trout melanocortin 2 receptors using heterologous MRAP1s［J］. General and Comparative Endocrinology，2011，174(1)：5-14.
［9］Kobayashi Y，Chiba H，Yamanome T，et al. Melanocortin receptor subtypes in interrenal cells and corticotropic activity of alpha-melanocyte-stimulating hormones in barfin flounder，Verasper moseri［J］. General and Comparative Endocrinology，2011，170(3)：558-568.
［10］Agulleiro M J，Sánchez E，Leal E，et al. Molecular characterization and functional regulation of melanocortin 2 receptor(MC2R)in the sea bass:A putative role in the adaptation to stress［J］. PloS One，2013，8(5)：e65450.
［11］Aluru N，Vijayan M M. Molecular characterization，tissue-specific expression，and regulation of melanocortin 2 receptor in rainbow trout［J］. Endocrinology，2008，149(9)：4577-4588.
［12］Ming J，Xie J，Xu P，et al. Molecular cloning and expression of two HSP70 genes in the Wuchang bream(Megalobrama amblycephala Yih)［J］. Fish & Shellfish Immunology，2010，28(3)：407-418.
［13］Zhao Y，Gul Y，Li S，et al. Cloning，identification and accurate normalization expression analysis of PPARa gene by GeNorm in Megalobrama amblycephala［J］. Fish & Shellfish Immunology，2011，31(3)：462-468.
［14］Mirzadegan T，Benko G，Filipek S，et al. Sequence analyses of G-protein-coupled receptors：similarities to rhodopsin［J］. Biochemistry，2003，42(10)：2759-2767.
［15］Tao Y X. The melanocortin-4 receptor：physiology，pharmacology，and pathophysiology［J］. Endocrine Reviews，2010，31(4)：506-543.
［16］Vandesompele J，Preter K D，Pattyn F，et al. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes［J］. Genome Biology，2002，3(7)：research0034.Ⅰ-0034.Ⅱ.
［17］Hoffmann C，Zürn A，Bünemann M，et al. Review：conformational changes in G-protein-coupled receptors—the quest for functionally selective conformations is open［J］. British Journal of Pharmacology，2008，153(S1)：S358-S366.
［18］Schith H B，Tesfaye A，Mutulis F，et al. Subtype selective binding properties of substituted linear melanocyte stimulating hormone analogues［J］. Neuropeptides，2002，36(6)：427-434.
［19］Schith H B，Yook P，Muceniece R，et al. Chimeric melanocortin MC1 and MC3 receptors：identification of domains participating in binding of melanocyte-stimulating hormone peptides［J］. Molecular Pharmacology，1998，54(1)：154-161.
［20］Reinick C L，Liang L，Angleson J K，et al. Identification of an MRAP-independent melanocortin-2 receptor：functional expression of the cartilaginous fish，Callorhinchus milii，melanocortin-2 receptor in CHO cells［J］. Endocrinology，2012，153(10)：4757-4765.
［21］Krasnov A，Koskinen H，Pehkonen P，et al. Gene expression in the brain and kidney of rainbow trout in response to handling stress［J］. BMC Genomics，2005，6(1)：3.
[HJ1.72mm] ［22］Mola L，Gambarelli A，Pederzoli A，et al. ACTH response to LPS in the first stages of development of the fish Dicentrarchus labrax L［J］. General and Comparative Endocrinology，2005，143(2)：99-103.
［23］Alsop D，Ings J S，Vijayan M M，et al. Adrenocorticotropic hormone suppresses gonadotropin-stimulated estradiol release from zebrafish ovarian follicles［J］. PLoS One，2009，4(7)：e6463.
［24］Xia Y，Wikberg J E. Localization of ACTH receptor mRNA by in situ hybridization in mouse adrenal gland［J］. Cell Tissue Research，1996，286(1)：63-68.
［25］Takeuchi S，Kudo T，Takahashi S. Molecular cloning of the chicken melanocortin 2(ACTH)-receptor gene［J］. Biochimica et Biophysica Acta-Molecular Cell Research，1998，1403(1)：102-108.
［26］Jacobs K，van Poucke M，Mattheeuws M，et al. Characterization of the porcine melanocortin 2 receptor gene(MC2R)［J］. Animal Genetics，2002，33(6)：415-421.
［27］Rotllant J，Tort L. Cortisol and glucose responses after acute stress by net handling in the sparid red porgy previously subjected to crowding stress［J］. Journal of Fish Biology，1997，51(1)：21-28.
［28］Fryer J N，Peter R E. Hypothalamic control of ACTH secretion in goldfish. Ⅲ. Hypothalamic cortisol implant studies［J］. General and Comparative Endocrinology，1977，33(2)：215-225.

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备注/Memo

备注/Memo:
收稿日期：2014-03-11
基金项目：国家现代农业产业技术体系建设专项资金(编号：CARS-46-10)；中央级公益性科研院所基本科研业务费专项资金(编号：2013JBFM10)。
作者简介：董晶晶(1989—)，女，河南周口人，硕士研究生，主要从事水产动物营养与疾病防控的研究。E-mail：jingzsmx@163.com。
通信作者：谢骏，研究员，主要从事池塘微生态学、水产动物的应激机制、细菌性病害生态防控技术的研究。E-mail：xiej@ffrc.cn。
更新日期/Last Update: 2014-06-25