|本期目录/Table of Contents|

[1]李丽丽,郎敬,杨洪一,等.大豆根际解磷菌的鉴定[J].江苏农业科学,2014,42(08):363-365.
 Li Lili,et al.Identification of phosphate-solubilizing bacteria in rhizosphere of soybean[J].Jiangsu Agricultural Sciences,2014,42(08):363-365.
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年08期
页码:
363-365
栏目:
资源与环境
出版日期:
2014-08-25

文章信息/Info

Title:
Identification of phosphate-solubilizing bacteria in rhizosphere of soybean
作者:
李丽丽123 郎敬4 杨洪一4 邰飞飞4 来永才1
1.黑龙江省农业科学院耕作栽培研究所,黑龙江哈尔滨 150086; 2.黑龙江省农业科学院博士后科研工作站,黑龙江哈尔滨 150086;3.黑龙江省林业科学研究所,黑龙江哈尔滨 150081; 4.东北林业大学生命科学学院,黑龙江哈尔滨 150040
Author(s):
Li Liliet al
关键词:
解磷菌溶磷圈16S rDNA大豆生物磷肥
Keywords:
-
分类号:
S154.3
DOI:
-
文献标志码:
A
摘要:
利用PVK平板,从大豆根际土壤中分离出8株解磷菌。培养分析结果显示,这8株解磷菌的溶磷圈均较大。其中,海伦Ⅲ号为革兰氏阴性菌,其余均为革兰氏阳性菌。形态学观察结果显示,北安Ⅰ号为链球菌,海伦Ⅱ号、海伦Ⅲ号为球菌,海伦Ⅳ号、北安Ⅲ号为杆菌。基于细菌核糖体16S rDNA序列对北安Ⅲ号菌株进行鉴定,结合形态特征确认该菌株为巨大芽孢杆菌,经研究发现该菌株是一种重要的微生物资源,可进一步开发为生物磷肥。
Abstract:
-

参考文献/References:

[1]李晓婷. 解磷细菌K3的GFP标记与在土壤中定殖及对玉米生长效应研究[D]. 南京:南京农业大学,2009:1-5.
[2]Gyaneshwar P,Naresh K G,Parekh L J,et al. Role of soil microorganisms in improving P nutrition of plants[J]. Plant and Soil,2002,245:83-93.
[3]Rodríguez H,Fraga R. Phosphate solubilizing bacteria and their role in plant growth promotion[J]. Biotechnology Advances,1999,17:319-339.
[4]万翠番,章昭琳,王报贵,等. 高黏附力双歧杆菌的筛选与鉴定[J]. 中国乳品工业,2012(5):13-15.
[5]Moreno C,Romero J,Romilio T E. Polymorphism in repeated 16S rRNA genes is a common property of type strains and environmental isolates of the genus Vibrio[J]. Microbiology,2002,148:1233-1239.
[6]Altschul S F,Madden TL,Chffer A A,et al. Gapped BLAST and PSI-BLAST:a new generation of protein database search programs[J]. Nucleic Acids Res,1997,25(17):3389-3402.
[7]Kucey R M N,Janzen H H,Legett M E. Microbially mediated increases in plant available phosphorus[J]. Advances in Agronomy,1989,42:199-228.

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备注/Memo

备注/Memo:
收稿日期:2014-02-06
基金项目:黑龙江省自然科学基金(编号:QC2011C110);黑龙江省哈尔滨市科技创新人才基金(编号:2012RFQYN023);中国博士后科学基金(编号:2011M500706、2012T50382);黑龙江省博士后科学基金(编号:LBH-Z11031);黑龙江省农业科学院博士后基金。
作者简介:李丽丽(1978—),女,辽宁阜新人,博士,助理研究员,主要从事微生物学研究。E-mail:lilili0622@126.com。
通信作者:杨洪一
更新日期/Last Update: 2014-08-25