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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第46卷

期数:

2018年07期

页码:

91-95

栏目:

植物保护

出版日期:

2018-04-05

文章信息/Info

Title:

Cloning of acetylcholinesterase gene from Myzus persicae and detection of S431F mutation frequency of Myzus persicae in different areas of China

作者:

刘晓岚¹;  田兆丰²;  李永丹¹

1.中国农业大学农学与生物技术学院，北京100193； 2.北京市农林科学院植物保护环境保护研究所，北京 100097

Author(s):

Liu Xiaolan; et al

关键词:

桃蚜; 乙酰胆碱酯酶; 基因克隆; cDNA; 变异检测

Keywords:

-

分类号:

S436.621.2⁺1；Q785

DOI:

-

文献标志码:

A

摘要:

克隆我国桃蚜乙酰胆碱酯酶基因(ace1、ace2)cDNA。我国桃蚜ace1经与NCBI(GenBank：AF287291.1)比对未发现氨基酸替换，ace2基因经cDNA末端快速克隆技术得到全长(NCBI已注册的ace2 GenBank：AY147797.1缺失3′ 端182 bp)，ace2全长为2 022 bp，编码674个氨基酸，序列已提交NCBI(GeneBank：KJ561353)。与NCBI(GenBank：AY147797.1)比对发现，我国桃蚜ace2除已报道的与桃蚜抗药性有关的S431F突变(氨基酸序列431位丝氨酸变为苯丙氨酸)外，在氨基酸序列55位处发现亮氨酸突变为丝氨酸(L55S)。利用突变检测技术，对2015、2016年采自我国河北、辽宁、江苏、青海等多个地区的桃蚜种群单头乙酰胆碱酯酶基因突变(S431F)频率进行检测。结果表明，2015、2016年各种群桃蚜单头乙酰胆碱酯酶基因突变(S431F)频率均大于85%，均已处于高水平突变，突变频率与生物测定抗性水平趋势一致。

Abstract:

-

参考文献/References:

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备注/Memo

备注/Memo:

收稿日期：2017-09-19
基金项目：国家科技支撑计划(编号：2012BAD19B01)。
作者简介：刘晓岚(1988—)，女，山东青岛人，硕士，科研助理，从事昆虫生理毒理研究。E-mail：lxldjj@163.com。
通信作者，李永丹，博士，副教授，硕士生导师，从事害虫综合治理研究。E-mail：yongdanli@cau.edu.cn。

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更新日期/Last Update: 2018-04-05