[1]张二豪,张杰.CRISPR/Cas9基因编辑技术应用于绿僵菌[J].江苏农业科学,2021,49(11):48-53.
　Zhang Erhao,et al.CRISPR/Cas9-mediated genome editing in Metarhizium acridum[J].Jiangsu Agricultural Sciences,2021,49(11):48-53.
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CRISPR/Cas9基因编辑技术应用于绿僵菌()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第49卷
期数:
2021年第11期
页码:
48-53
栏目:
生物技术
出版日期:
2021-06-05

文章信息/Info

Title:
CRISPR/Cas9-mediated genome editing in Metarhizium acridum
作者:
张二豪1; 张杰2
1.西藏农牧学院食品科学学院，西藏林芝 860000； 2.周口师范学院生命科学与农学学院，河南周口 466001
Author(s):
Zhang Erhao; et al
关键词:
CRISPR/Cas9; 重组酶; 基因编辑; 绿僵菌
Keywords:
-
分类号:
S188
DOI:
-
文献标志码:
A
摘要:
近年来的研究显示，CRISPR/Cas9系统是强有力的基因编辑新技术。以蝗绿僵菌为试验对象，以同源重组敲除系统为对照，研究CRISPR/Cas9系统敲除蝗绿僵菌的基因isp4核苷酸序列。阐明了CRISPR/Cas9载体构建的方法，比较了CRISPR/Cas9和Recombinase敲除技术的异同，最后通过PCR和突变菌株的表型验证了CRISPR/Cas9系统能够应用于绿僵菌。结果表明，CRISPR/Cas9在昆虫病原真菌绿僵菌中是有效的基因编辑技术。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2020-10-09
基金项目：周口师范学院高层次人才科研启动经费研究项目(编号：2018B180061)；西藏自治区自然科学基金 (编号：XZ2018ZRG-19)。
作者简介：张二豪(1989—)，男，河南平顶山人，硕士，讲师，主要研究方向为分子生物学。E-mail：1158496424@qq.com。
通信作者：张杰，博士，讲师，主要研究方向为分子生物学与生物工程。E-mail：Zhangjiezk@qq.com。
更新日期/Last Update: 2021-06-05