[1]杨威,闫海霞,刘雨昕,等.芽孢杆菌1JN2产胞外多糖协助寄主植物缓解镉胁迫[J].江苏农业科学,2022,50(6):211-216.
Yang Wei,et al.Study on mechanism of exopolysaccharides produced by Bacillus subtilis 1JN2 to alleviate Cd2+ stress for host plants[J].Jiangsu Agricultural Sciences,2022,50(6):211-216.
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芽孢杆菌1JN2产胞外多糖协助寄主植物缓解镉胁迫(PDF)
Yang Wei,et al.Study on mechanism of exopolysaccharides produced by Bacillus subtilis 1JN2 to alleviate Cd2+ stress for host plants[J].Jiangsu Agricultural Sciences,2022,50(6):211-216.
《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]
- 卷:
- 第50卷
- 期数:
- 2022年第6期
- 页码:
- 211-216
- 栏目:
- 资源与环境
- 出版日期:
- 2022-03-20
文章信息/Info
- Title:
- Study on mechanism of exopolysaccharides produced by Bacillus subtilis 1JN2 to alleviate Cd2+ stress for host plants
- 作者:
- 杨威1; 闫海霞2; 刘雨昕1; 周丹妮1; 董光辉1; 李正鹏1; 罗玉明1
- 1.淮阴师范学院生命科学学院/江苏省环洪泽湖生态农业生物技术重点实验室，江苏淮安 223300；2.淮安市农业技术推广中心，江苏淮安 223300
- Keywords:
- -
- 分类号:
- S182
- DOI:
- -
- 文献标志码:
- A
- 摘要:
- 笔者所在课题组前期筛选到1株芽孢杆菌，能够通过胞外分泌物吸附作用协助寄主植物缓解镉离子胁迫。为了深入研究该菌株吸附镉离子的胞外分泌物组分，构建菌株胞外多糖缺失突变体，首先通过共培养试验比较野生型及突变体菌株在镉离子处理后的生长量变化、镉离子吸附能力；其次通过温室试验评价菌株协助寄主缓解镉胁迫效果及菌株定殖量变化；最后通过扫描电镜比较镉离子共培养后的野生型菌株和突变体菌体及胞外分泌物形态。结果表明，镉离子处理后野生型与突变体菌株生长量无明显差异，但是突变体菌株对镉离子吸附性明显降低。在以番茄作为寄主进行的温室试验中，野生型与突变体菌株定殖量在镉离子处理后先下降后上升，在移栽后21 d与对照组无明显差异，二者均能够增强寄主植物的耐受性，与空白对照相比植物鲜质量分别增加12%、38%，叶绿素含量分别增加14%、1%，根系活力分别增加507%、233%，叶片相对电导率分别降低41%、58%。但是突变体菌株处理组相对于野生型番茄茎中镉离子浓度提高了84%。另外，在镉离子共培养12、24、36、48 h后分别收集菌体，通过扫描电镜发现野生型和突变体菌株在镉离子处理后24 h开始菌体能够进行自我复原。说明菌株1JN2主要通过胞外多糖吸附作用降低镉离子在寄主体内积累从而协助寄主植物缓解镉胁迫。
- Abstract:
- -
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备注/Memo
- 备注/Memo:
-
收稿日期：2021-07-07
基金项目：江苏省高校自然科学基金(编号：21KJA210005)；江苏省自然科学基金(编号：BK20170467)；江苏省大学生创新训练计划(编号：201910323013Z、202110323060Y)。
作者简介：杨威(1983—)，男，河北承德人，博士，副教授，主要从事生物防治研究。E-mail：yangw107@126.com。
通信作者：罗玉明，教授，主要从事生物防治研究。E-mail：yumingluo@163.com。
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