[1]姜泽锴,徐宁,王明海,等.干旱胁迫24 h不同绿豆品种的转录组分析[J].江苏农业科学,2025,53(22):66-72.
 Jiang Zekai,et al.Transcriptome analysis of different mung bean cultivars under drought stress for 24 hours[J].Jiangsu Agricultural Sciences,2025,53(22):66-72.
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干旱胁迫24 h不同绿豆品种的转录组分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第22期
页码:
66-72
栏目:
生物技术
出版日期:
2025-11-20

文章信息/Info

Title:
Transcriptome analysis of different mung bean cultivars under drought stress for 24 hours
作者:
姜泽锴12徐宁2王明海2邓昆鹏2兰天娇2程钰鑫2包淑英2窦金光2韩丹2徐亚鹏3王涵12郭中校12
1.吉林农业大学农学院,吉林长春 130118; 2.吉林省农业科学院作物资源研究所,吉林公主岭 136100; 3.延边大学农学院,吉林延吉 133000
Author(s):
Jiang Zekaiet al
关键词:
绿豆干旱胁迫萌发期基因表达功能富集分析
Keywords:
-
分类号:
S522.01
DOI:
-
文献标志码:
A
摘要:
为了筛选出与绿豆抗旱性相关的差异表达基因,从277份绿豆种质资源中选出耐旱性良好的绿丰2号和耐旱性较差的LD0146,采用24%的PEG-6000溶液模拟干旱胁迫,在24 h后对其萌发期样本进行转录组测序(RNA-Seq)分析。基于差异倍数 |log2(Fold Change)|≥1且P≤0.05的筛选标准,获得若干显著差异表达基因(DEG),这些基因涉及氨基酸代谢过程、氧化压力响应和碳代谢等重要代谢途径,其中jg18845、jg2723、jg143这3个差异表达基因已被证明与抗旱性有关。在基因本体(GO)数据库中,差异表达基因显著富集于代谢过程和生物合成等生物过程。在京都基因与基因组百科全书(KEGG)数据库中,差异表达基因显著富集于光合作用、碳代谢等关键代谢途径。研究结果表明,耐旱绿豆品种通过调节这些关键代谢途径和基因表达,在干旱胁迫下维持了生长和代谢活动。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2024-12-24
基金项目:国家现代农业产业技术体系建设专项(编号:CARS-08-Z8)。
作者简介:姜泽锴(2000—),男,山东济南人,硕士研究生,主要研究方向为作物遗传育种。E-mail:135167933@qq.com。
通信作者:郭中校,博士,研究员,主要研究方向为食用豆种质资源耐旱、耐盐碱、抗豆象等鉴评与种质创新及食用豆高产优质新品种选育及栽培技术。E-mail:guozhx@cjaas.com。
更新日期/Last Update: 2025-11-20