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[1]董晶晶,薛春雨,习丙文,等.团头鲂黑素皮质素受体2基因克隆、组织分布和应激后的表达分析[J].江苏农业科学,2014,42(06):20-26.
　Dong Jingjing,et al.Gene cloning，tissue distribution and expression after stress of melanocortin receptor 2 in Megalobrama amblycephala[J].Jiangsu Agricultural Sciences,2014,42(06):20-26.
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团头鲂黑素皮质素受体2基因克隆、组织分布和应激后的表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年06期
页码:
20-26
栏目:
生物技术
出版日期:
2014-06-25

文章信息/Info

Title:
Gene cloning，tissue distribution and expression after stress of melanocortin receptor 2 in Megalobrama amblycephala
作者:
董晶晶1; 2;  薛春雨1; 2;  习丙文1;  梁利国2;  刘波2;  任鸣春1;  谢骏1; 2
1.南京农业大学无锡渔业学院，江苏 无锡 214081；2.中国水产科学研究院淡水渔业研究中心/农业部淡水渔业和种质资源利用重点实验室，江苏无锡 214081
Author(s):
Dong Jingjing; et al
关键词:
团头鲂; MC2R基因; 皮质醇; 克隆; 组织表达; 应激
Keywords:
-
分类号:
S917
DOI:
-
文献标志码:
A
摘要:
在神经内分泌下丘脑-垂体-肾间组织轴调控中，黑素皮质素受体2(MC2R)与垂体释放的促肾上腺皮质激素结合而激活相应的cAMP信号转导途径，对鱼类的应激反应调控具有重要的作用。本研究通过PCR、RACE技术克隆获得团头鲂MC2R mRNA序列，并通过实时定量PCR分析了团头鲂MC2R组织分布情况及捕捞胁迫后的表达变化。结果显示，获得的MC2R mRNA序列开放阅读框为915 bp，编码304个氨基酸。序列同源性及系统育分析显示，团头鲂MC2R氨基酸序列与鲤科鱼类的MC2R聚为一支，与金鱼(Carassius auratus)MC2R的氨基酸序列同源性最高(87%)。组织分布结果表明，头肾中MC2R的表达量最高，脾脏、精巢和脑中有相对较高的表达量，中肾、肝、肠和肌肉等MC2R的表达量相对较低。经捕捞出水体60 s的急性应激处理后，皮质醇在4 h时显著升高，24 h时表现出下降的趋势；MC2R基因表达量在头肾中变化不显著，然而脾、脑和精巢中MC2R 在4 h时表达量相对于1 h则呈现出显著的下降。通过对团头鲂应激后主要调控因子变化规律的研究，可为鱼类应激后调控措施的实施提供参考依据。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2014-03-11
基金项目：国家现代农业产业技术体系建设专项资金(编号：CARS-46-10)；中央级公益性科研院所基本科研业务费专项资金(编号：2013JBFM10)。
作者简介：董晶晶(1989—)，女，河南周口人，硕士研究生，主要从事水产动物营养与疾病防控的研究。E-mail：jingzsmx@163.com。
通信作者：谢骏，研究员，主要从事池塘微生态学、水产动物的应激机制、细菌性病害生态防控技术的研究。E-mail：xiej@ffrc.cn。
更新日期/Last Update: 2014-06-25