|本期目录/Table of Contents|

[1]贺建忠,陈宏伟,王贵强,等.金黄色葡萄球菌生物被膜形成与生物被膜相关基因的调查研究[J].江苏农业科学,2015,43(04):55-59.
　He Jianzhong,et al.Investigation of biofilm formation and biofilm-associated genes in Staphylococcus aureus[J].Jiangsu Agricultural Sciences,2015,43(04):55-59.
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金黄色葡萄球菌生物被膜形成与生物被膜
相关基因的调查研究(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第43卷
期数:
2015年04期
页码:
55-59
栏目:
生物技术
出版日期:
2015-04-25

文章信息/Info

Title:
Investigation of biofilm formation and biofilm-associated genes in Staphylococcus aureus
作者:
贺建忠1;  陈宏伟1;  王贵强2;  王永1;  白万胜1;  郭新怀1;  王勇1
1.新疆生产建设兵团塔里木畜牧科技重点实验室，新疆阿拉尔 843300； 2.湖北省畜禽育种中心，湖北武汉 430070
Author(s):
He Jianzhong; et al
关键词:
金黄色葡萄球菌; 生物被膜; 奶牛乳房炎; 生物被膜相关基因
Keywords:
-
分类号:
S852.61+1
DOI:
-
文献标志码:
A
摘要:
以分离自全国9个省(市、区)的102株奶牛乳房炎源性金黄色葡萄球菌为研究对象，应用刚果红法(congo red agar，CRA)和半定量黏附试验(semi quantitative adherence assay，SQAA)检测了生物被膜形成情况，应用PCR法检测了8种生物被膜相关基因分布情况。结果发现，携带7种基因的菌株占有绝对优势，其次是携带6种基因的菌株；最流行的基因组合是SigB-icaR-icaA-icaD-sarA-rbf-SasG，比例高达66.7%；北京、内蒙古、宁夏、甘肃、广西、上海等地生物被膜形成与生物被膜相关基因的分布高度一致。以上研究结果表明，多种生物被膜相关基因组合是奶牛乳房炎源性金黄色葡萄球菌流行的主要特点，生物被膜形成和生物被膜相关基因的分布在大多数地区表现高度一致性，rbf和SigB基因可能在金黄色葡萄球菌生物被膜形成和乳房感染过程中发挥着重要作用。本研究结果将为金黄色葡萄球菌性乳房炎的防治提供一定的科学参考。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2014-04-15
基金项目：国家自然科学基金地区基金(编号：31260628)；塔里木畜牧科技兵团重点实验室开放课题(编号：HS201204)。
作者简介：贺建忠(1977—)，男，内蒙古五原人，硕士，副教授，研究方向为临床兽医学。E-mail：talimuhe_he@126.com。
通信作者：白万胜，副教授，研究方向为临床兽医学。E-mail：bwsdky@126.com。
更新日期/Last Update: 2015-04-25