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[1]代其林,王金玲,马明莉,等.NaCl胁迫处理对豇豆幼苗抗氧化酶活性的影响[J].江苏农业科学,2015,43(10):193-196.
　Dai Qilin,et al.Effect of NaCl stress on antioxidant enzymes activity of cowpea (Vigna unguiculata Linn.) seedlings[J].Jiangsu Agricultural Sciences,2015,43(10):193-196.
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NaCl胁迫处理对豇豆幼苗抗氧化酶活性的影响(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第43卷
期数:
2015年10期
页码:
193-196
栏目:
园艺与林学
出版日期:
2015-10-25

文章信息/Info

Title:
Effect of NaCl stress on antioxidant enzymes activity of cowpea (Vigna unguiculata Linn.) seedlings
作者:
代其林1;  王金玲2;  马明莉1;  吕旭才1;  郭翠1;  王劲3;  杜世章2
1. 西南科技大学生命科学与工程学院，四川绵阳 621000； 2.绵阳师范学院，四川绵阳 621000；
3.中国农业科学研究院生物技术研究所，北京 100081
Author(s):
Dai Qilin; et al
关键词:
NaCl胁迫; 豇豆幼苗; 抗氧化酶; 可溶性蛋白; 脯氨酸; 丙二醛; 实时定量PCR
Keywords:
-
分类号:
S643.401
DOI:
-
文献标志码:
A
摘要:
主要研究150 mmol/L NaCl胁迫处理对豇豆(Vigna unguiculata Linn.)幼苗叶片抗氧化酶活性的影响。结果表明，豇豆幼苗在受到NaCl胁迫后，其叶片内可溶性蛋白含量、脯氨酸含量、丙二醛含量随胁迫时间的延长均逐渐升高，胁迫12 h后，它们的含量都达到峰值；在150 mmol/L NaCl胁迫过程中，豇豆幼苗叶片的抗氧化酶如超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)等活性在0～12 h期间逐渐上升，12 h后其活性也均达到最高值；在12~48 h胁迫期间，3种抗氧化酶活性逐渐下降，但仍强于非盐胁迫下的抗氧化酶活性。同时，对NaCl胁迫下这3种抗氧化酶基因的相对表达水平进行荧光定量分析，结果表明，SOD和CAT 等2种抗氧化酶基因相对转录表达水平与胁迫期间内相应酶的活性变化一致，而POD基因表达在胁迫6 h后就已经达到最大值，与其酶活性在12 h后达最大值是不一致的。分析结果表明，在盐胁迫下NaCl诱导了SOD、POD、CAT等 3种酶基因的表达，抗氧化酶活性相应增强，从而增强了豇豆幼苗应对NaCl胁迫的能力，为盐碱地培育豇豆作物具有一定指导意义。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2014-09-28
基金项目：国家“973”计划(编号：2013CB733903)；国家“863”计划(编号：2012AA063503)；国家转基因专项(编号：2014ZX0801201B)；公益性行业(农业)科研专项 (编号：201103007)；西南科技大学博士研究基金(编号：11zx7104)。
作者简介：代其林(1970—)，男，重庆人，博士，副教授，主要从事植物逆境生理与分子生物学研究。E-mail：daiqilinmj@sina.com。
通信作者：杜世章，博士，教授，主要从事分子生物学研究。E-mail：wjdsz@vip.sina.com。
更新日期/Last Update: 2015-10-25