|本期目录/Table of Contents|

[1]韩兴,刘亚南,甄丹妹,等.玫瑰黄链霉菌Men-myco-93-63产抗生素发酵培养基的优化[J].江苏农业科学,2017,45(10):100-103.
 Han Xing,et al.Optimization of antibiotic-producing fermentation medium of Streptomyces roseoflavus Men-myco-93-63[J].Jiangsu Agricultural Sciences,2017,45(10):100-103.
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玫瑰黄链霉菌Men-myco-93-63产抗生素
发酵培养基的优化
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年10期
页码:
100-103
栏目:
植物保护
出版日期:
2017-05-20

文章信息/Info

Title:
Optimization of antibiotic-producing fermentation medium of Streptomyces roseoflavus Men-myco-93-63
作者:
韩兴 刘亚南 甄丹妹 刘大群 赤国彤
河北农业大学植物保护学院/河北省农作物病虫害生物防治工程技术研究中心/国家北方山区农业工程技术研究中心,河北保定 071001
Author(s):
Han Xinget al
关键词:
玫瑰黄链霉菌Men-myco-93-63发酵抗生素响应面分析
Keywords:
-
分类号:
S476+.8
DOI:
-
文献标志码:
A
摘要:
以玫瑰黄链霉菌Men-myco-93-63为发酵菌株,采用Plackett-Burman试验设计、最陡爬坡试验、中心复合试验设计和响应面分析法,以抗生素产量为响应值,对其发酵培养基进行优化。首先,采用Plackett-Burman设计筛选出具有正显著效应的花生饼粉和玉米浆。其次,通过最陡爬坡试验逼近最大响应区域。最后,采用中心复合试验对这2个因素进行优化。最终确定发酵的最适培养基含量配方为葡萄糖2%,可溶性淀粉1%,花生饼粉4.96%,玉米浆2.66%,氯化钠0.4%,碳酸钙0.3%,磷酸二氢钾0.02%。优化后发酵培养基抗生素产量为2 030.6 mg/L,比优化前的771.9 mg/L 提高了1.63倍。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2016-02-26
基金项目:国家自然科学基金(编号:31572050);河北省中药材产业技术体系(编号:1004029)。
作者简介:韩兴(1990—),男,河北石家庄人,硕士研究生,主要从事天然产物农药研究。E-mail:15100201526@163.com。
通信作者:赤国彤,硕士,教授,硕士生导师,主要从事农药剂型加工研究,E-mail:chiguotong@126.com;刘大群,博士,教授,博士生导师,主要从事植物病理学研究,E-mail:ldq@hebau.edu.cn。
更新日期/Last Update: 2017-05-20