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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第46卷

期数:

2018年07期

页码:

91-95

栏目:

植物保护

出版日期:

2018-04-05

文章信息/Info

Title:

Cloning of acetylcholinesterase gene from Myzus persicae and detection of S431F mutation frequency of Myzus persicae in different areas of China

作者:

刘晓岚¹;  田兆丰²;  李永丹¹

1.中国农业大学农学与生物技术学院，北京100193； 2.北京市农林科学院植物保护环境保护研究所，北京 100097

Author(s):

Liu Xiaolan; et al

关键词:

桃蚜; 乙酰胆碱酯酶; 基因克隆; cDNA; 变异检测

Keywords:

-

分类号:

S436.621.2⁺1；Q785

DOI:

-

文献标志码:

A

摘要:

克隆我国桃蚜乙酰胆碱酯酶基因(ace1、ace2)cDNA。我国桃蚜ace1经与NCBI(GenBank：AF287291.1)比对未发现氨基酸替换，ace2基因经cDNA末端快速克隆技术得到全长(NCBI已注册的ace2 GenBank：AY147797.1缺失3′ 端182 bp)，ace2全长为2 022 bp，编码674个氨基酸，序列已提交NCBI(GeneBank：KJ561353)。与NCBI(GenBank：AY147797.1)比对发现，我国桃蚜ace2除已报道的与桃蚜抗药性有关的S431F突变(氨基酸序列431位丝氨酸变为苯丙氨酸)外，在氨基酸序列55位处发现亮氨酸突变为丝氨酸(L55S)。利用突变检测技术，对2015、2016年采自我国河北、辽宁、江苏、青海等多个地区的桃蚜种群单头乙酰胆碱酯酶基因突变(S431F)频率进行检测。结果表明，2015、2016年各种群桃蚜单头乙酰胆碱酯酶基因突变(S431F)频率均大于85%，均已处于高水平突变，突变频率与生物测定抗性水平趋势一致。

Abstract:

-

参考文献/References:

［1］Fournier D，Mutero A. Modification of acetylcholinesterase as a mechanism of resistance to insecticides［J］. Comparative Biochemistry and Physiology Part C：Pharmacology，Toxicology and Endocrinology，1994，108(1)：19-31.
［2］Fournier D. Mutations of acetylcholinesterase which confer insecticide resistance in insect populations［J］. Chemico-Biological Interactions，2005，157/158：257-261.
［3］李显春，王荫长. 昆虫抗药性靶标不敏感机制的研究进展［J］. 昆虫学报，1998，41(4)：417-422.
［4］林建国，张传溪，唐振华. 与昆虫抗药性相关的乙酰胆碱酯酶基因突变研究进展［J］. 农药学学报，2005，7(1)：1 - 6.
［5］Li F，Han Z J. Mutations in acetylcholinesterase associated with insecticide resistance in the cotton aphid，Aphis gossypii Glover［J］. Insect Biochemistry and Molecular Biology，2004，34(4)：397-405.
［6］Chen Z，Newcomb R，Forbes E，et al. The acetylcholinesterase gene and organophosphorus resistance in the Australian sheep blowfly，Lucilia cuprina［J］. Insect Biochemistry and Molecular Biology，2001，31(8)：805-816.
［7］Anazawa Y，Tomita T，Aiki Y，et al. Sequence of a cDNA encoding acetylcholinesterase from susceptible and resistant two-spotted spider mite，Tetranychus urticae［J］. Insect Biochemistry and Molecular Biology，2003，33(5)：509 - 514.
［8］Russell R J，Claudianos C，Campbell P M，et al. Two major classes of target site insensitivity mutations confer resistance to organophosphate and carbamate insecticides［J］. Pesticide Biochemistry and Physiology，2004，79(3)：84 - 93.
［9］Moores G D，Devine G J，Devonshire A L. Insecticide-insensitive acetylcholinesterase can enhance esterase-based resistance in Myzus persicae and Myzus nicotianae［J］. Pesticide Biochemistry and Physiology，1994，49(2)：114-120.
［10］Moores G D，Devine G J，Devonshire A L. Insecticide resistance due to insensitive acetylcholinesterase in Myzus persicae and Myzus nicotianae［C］// Brighton Crop Protection Conference：pests and diseases. England：British Crop Protection Council，1994，3(3)：413-418.
［11］Foster S P，Denholm I，Harling Z K，et al. Intensification of insecticide resistance in UK field populations of the peach-potato aphid，Myzus persicae(Hemiptera：Aphididae) in 1996［J］. Bulletin of Entomological Research，1998，88(2)：127-130.
［12］Foster S P，Devonshire A L. Field-simulator study of insecticide resistance conferred by esterase-，MACE- and kdr-based mechanisms in the peach-potato aphid，Myzus persicae(Sulzer)［J］. Pesticide Management Science，1999，55(8)：810-814.
［13］Srigiriraju L，Semtner P J，Anderson T D，et al. Monitoring for MACE resistance in the tobacco-adapted form of the green peach aphid，Myzus persicae(Sulzer)(Hemiptera：Aphididae) in the eastern United States［J］. Crop protection，2010，29(2)：197-202.
［14］Mutero A，Pralavorio M，Bride J M，et al. Resistance-associated point mutations in insecticide-insensitive acetylcholinesterase［J］. Proceedings of the National Academy of Sciences，1994，91(13)：5922-5926.
［15］Devonshire A L，Byrne F J，Moores G D，et al. Biochemical and molecular characterisation of insecticide insensitive acetylcholinesterase in resistant insects［M］// Doctor B P，Taylor P，Quinn D M，et al. Structure and function of cholinesterases and related proteins. Boston：Springer，1998：491-496.
［16］Field L M，Anderson A P，Denholm L，et al. Use of biochemical and DNA diagnostics for characterising multiple mechanisms of insecticide resistance in the peach-potato aphid，Myzus persicae (Sulzer)［J］. Pesticide Management Science，1997，51(3)：283-289.
［17］柏建，周小毛，方勇. 桃蚜抗药性监测［J］. 农药研究与应用，2009(6)：27-29.
［18］Foster S P，Denholm I，Devonshire A L. Field-simulator studies of insecticide resistance to dimethylcarbamates and pyrethroids conferred by metabolic- and target site-based mechanisms in peach-potato aphids，Myzus persicae(Hemiptera：Aphididae)［J］. Pest Management Science，2002，58(8)：811-816.
［19］Sawicki R M，Rice A D. Response of susceptible and resistant peach-potato aphids Myzus persicae(Sulzer) to insecticides in leaf-dip bioassays［J］. Pesticide Management Science，1978，9(6)：513-516.
［20］宫亚军，王泽华，石宝才，等，北京地区不同桃蚜种群的抗药性研究［J］. 中国农业科学，2011，44(21)：4385-4394.
［21］顾春波，王开运，辛海军，等. ，我国中部烟区(桃)蚜［Myzus persicae (Sulzer)］的抗药性研究［J］. 山东农业大学学报(自然科学版)，2007，38(1)：11-14.
［22］Nabeshima T，Kozaki T，Tomita T，et al.，An amino acid substitution on the second acetylcholinesterase in the pirimicarb-resistant strains of the peach potato aphid，Myzus persicae［J］. Biochemical and Biophysical Research Communications，2003，307(1)：15-22.

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备注/Memo

备注/Memo:

收稿日期：2017-09-19
基金项目：国家科技支撑计划(编号：2012BAD19B01)。
作者简介：刘晓岚(1988—)，女，山东青岛人，硕士，科研助理，从事昆虫生理毒理研究。E-mail：lxldjj@163.com。
通信作者，李永丹，博士，副教授，硕士生导师，从事害虫综合治理研究。E-mail：yongdanli@cau.edu.cn。

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更新日期/Last Update: 2018-04-05