|本期目录/Table of Contents|

[1]袁巧云,孙悦,张迹.多菌灵降解菌株djl-10的分离及降解特性[J].江苏农业科学,2019,47(23):284-288.
 Yuan Qiaoyun,et al.Isolation and characterization of a carbendazim-degrading strain djl-10[J].Jiangsu Agricultural Sciences,2019,47(23):284-288.
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多菌灵降解菌株djl-10的分离及降解特性(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第23期
页码:
284-288
栏目:
资源与环境
出版日期:
2019-12-30

文章信息/Info

Title:
Isolation and characterization of a carbendazim-degrading strain djl-10
作者:
袁巧云 孙悦 张迹
淮阴师范学院生命科学学院/江苏省环洪泽湖生态农业生物技术重点实验室,江苏淮安 223300
Author(s):
Yuan Qiaoyunet al
关键词:
分枝杆菌多菌灵生物降解废水处理系统
Keywords:
-
分类号:
X172
DOI:
-
文献标志码:
A
摘要:
从多菌灵生产废水处理系统中,通过富集和选择性培养,分离得到1株能高效降解多菌灵的细菌,并将其命名为djl-10。根据菌株的菌落形态,生理生化特性及基于16S rDNA序列的系统发育分析等,初步将菌株鉴定为分枝杆菌属。该菌株能利用多菌灵作为唯一碳源、氮源进行生长并基本彻底矿化多菌灵。2-氨基苯并咪唑和2-羟基苯并咪唑为菌株降解多菌灵的中间代谢产物。菌株能够在较宽温度和pH值范围内有效降解多菌灵,其降解多菌灵的最适温度和pH值分别为37 ℃、7.0。装液量试验结果表明,菌株djl-10对多菌灵的降解明显依赖氧气。Ca2+、Mg2+、Fe3+等离子能够明显促进菌株djl-10对多菌灵的降解。此外,本研究克隆和表达菌株djl-10的多菌灵水解酶基因mhe。酶促反应结果表明,纯化的重组酶Mhe对多菌灵具有明显的催化活性。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期:2018-09-03
基金项目:国家自然科学基金(编号:31300099)。
作者简介:袁巧云(1981—),女,江苏盐城人,硕士,主要从事微生物学研究。E-mail:qiaoyunyuan@163.com。
通信作者:张迹,博士,讲师,主要从事环境微生物学研究。E-mail:zhangjihnu@163.com。
更新日期/Last Update: 2019-12-05