|本期目录/Table of Contents|

[1]赵静,常继东,郑丽君,等.对羟基苯甲酸对尖孢镰刀菌生物学特性及侵染毒力的影响[J].江苏农业科学,2020,48(22):291-294.
 Zhao Jing,et al.Effects of p-hydroxybenzoic acid on biological characteristics and infection toxicity of Fusarium oxysporum[J].Jiangsu Agricultural Sciences,2020,48(22):291-294.
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对羟基苯甲酸对尖孢镰刀菌生物学特性及侵染毒力的影响(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第48卷
期数:
2020年第22期
页码:
291-294
栏目:
资源与环境
出版日期:
2020-11-20

文章信息/Info

Title:
Effects of p-hydroxybenzoic acid on biological characteristics and infection toxicity of Fusarium oxysporum
作者:
赵静1 常继东2 郑丽君3 王志远4
1.玉溪师范学院化学生物与环境学院,云南玉溪 653100; 2.云南省新平县杨武镇卫生院,云南新平 653400;3.云南省玉溪市质量技术监督综合检测中心,云南玉溪 653100; 4.德宏师范高等专科学校生命科学系,云南德宏 678400
Author(s):
Zhao Jinget al
关键词:
对羟基苯甲酸尖孢镰刀菌孢子活性纤维素酶
Keywords:
-
分类号:
S182
DOI:
-
文献标志码:
A
摘要:
明确对羟基苯甲酸对尖孢镰刀菌侵染毒力的影响,可为克服三七连作障碍提供有效的策略。结合荧光素二乙酸酯-碘化丙啶(FDA-PI)染色及马铃薯葡萄糖琼脂(PDA)平板萌发法,分析不同浓度下对羟基苯甲酸与尖孢镰刀菌孢子活性的相关性;采用酶学法分析不同浓度下对羟基苯甲酸对真菌纤维素酶分泌的影响。FDA-PI染色结果表明,当对羟基苯甲酸浓度为5 mmol/L时,尖孢镰刀菌产孢数量最多,孢子活性最强。PDA平板萌发测定结果显示,对羟基苯甲酸浓度为1 mmol/L时,尖孢镰刀菌孢子的平均萌发率为58.05%,孢子萌发率最高。低浓度的对羟基苯甲酸胁迫下,尖孢镰刀菌分泌的纤维素酶活性增加,而高浓度下降低;对羟基苯甲酸浓度为5 mmol/L时,纤维素酶活性最高,峰值出现在培养4 d时,达到 1.57 U/mL,是相同时间下对照的5.6倍。研究表明,低浓度对羟基苯甲酸(1~5 mmol/L)胁迫有利于尖孢镰刀菌孢子生长,且提高其分泌的纤维素酶活性。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2020-03-13
基金项目:云南省科技厅青年基金(编号:2017FD160)。
作者简介:赵静(1983—),女,云南玉溪人,博士,副教授,主要从事作物病虫害与防治研究。E-mail:hujun_maolv@163.com。
通信作者:王志远,博士,副教授,主要从事作物病虫害与防治研究。E-mail:yuan33441@sina.com。
更新日期/Last Update: 2020-11-20