|本期目录/Table of Contents|

[1]郄卫那,张兰英,何健,等.1株饲用木聚糖酶产生菌的分离、鉴定、发酵、酶学性质及应用[J].江苏农业科学,2014,42(10):196-200.
　Qie Weina,et al.Isolation，identification，fermentation，enzymatic properties， and application of an xylanase-producing strain for feed[J].Jiangsu Agricultural Sciences,2014,42(10):196-200.
点击复制

1株饲用木聚糖酶产生菌的分离、鉴定、发酵、酶学性质及应用(PDF)
分享到：

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年10期
页码:
196-200
栏目:
畜牧兽医
出版日期:
2014-10-25

文章信息/Info

Title:
Isolation，identification，fermentation，enzymatic properties， and application of an xylanase-producing strain for feed
作者:
郄卫那1;  张兰英2;  何健1;  贺芹1
1.南京农业大学生命科学学院/农业部农业环境微生物工程重点开放实验室，江苏南京 210095；2.江西省南康市第一小学，江西南康 341400
Author(s):
Qie Weina; et al
关键词:
木聚糖酶; 酶学性质; 产酶条件; 饲料; 尖孢镰刀菌
Keywords:
-
分类号:
S182
DOI:
-
文献标志码:
A
摘要:
以木聚糖为唯一碳源，从长期堆放枯枝败叶的土壤中筛选能够高效降解木聚糖的菌株，并对该菌的产酶条件和酶学性质进行研究。分离得到1株酶活性较高且产酶相对稳定的菌株，鉴定为尖孢镰刀菌，命名为Fusarium oxysporum MJ23。研究表明，木聚糖、酵母粉是该菌的最适培养底物，在最适培养条件下，该菌株所产木聚糖酶活力可达186 U/mL；木聚糖酶的最适作用温度、pH值分别为55 ℃、6，55 ℃保温3 h，酶活力仍可维持90%以上；Ca2+、Fe2+、Mn2+、Tween 80和聚乙二醇对木聚糖酶活力有较强促进作用，Cu2+、Zn2+、EDTA、尿素和SDS则会抑制酶活，其中SDS的抑制作用最强。模拟动物体内对饲料中半纤维素组分的消化，木聚糖酶的添加可使猪饲料中木糖生成量提高28%，鸡饲料中木糖生成量提高16%，表明该菌株所产木聚糖酶具有用作饲料添加剂的可能性。
Abstract:
-

参考文献/References:

［1］Collins T，Gerday C，Feller G. Xylanases，xylanase families and extremophilic xylanases［J］. FEMS Microbiology Reviews，2005，29 (1)：3-23.
［2］Subramaniyan S，Prema P. Biotechnology of microbial xylanases：enzymology，molecular biology，and application［J］. Critical Reviews in Biotechnology，2002，22(1)：33-64.
［3］Akpinar O，Erdogan K，Bostanci S. Production of xylooligosaccharides by controlled acid hydrolysis of lignocellulosic materials［J］. Carbohydrate Research，2009，344(5)：660-666.
［4］Cubero O F，Crespo A，Fatehi J，et al. DNA extraction and PCR amplification method suitable for fresh，herbarium-stored，lichenized，and other fungi［J］. Plant Systematics and Evolution，1999，216(3/4)：243-249.
［5］Gupta N，Reddy V S，Maiti S，et al. Cloning，expression，and sequence analysis of the gene encoding the alkali-stable，thermostable endoxylanase from alkalophilic，mesophilic Bacillus sp. strain NG-27［J］. Applied and Environmental Microbiology，2000，66(6)：2631-2635.
［6］GB/T 23874—2009饲料添加剂木聚糖酶活力的测定分光光度法［S］. 北京：中国标准出版社.
［7］崔细鹏，周平发，王敏，等. 饲料原料为底物一种木聚糖酶体外酶解的方法［J］. 广东饲料，2012，21(2)：37-39.
［8］Demirba A. Gaseous products from biomass by pyrolysis and gasification：effects of catalyst on hydrogen yield［J］. Energy Conversion and Management，2002，43(7)：897-909.
［9］Moghtaderi B. Effects of controlling parameters on production of hydrogen by catalytic steam gasification of biomass at low temperatures［J］. Fuel，2007，86(15)：2422-2430.
［10］王在贵，张宏福，张永跃，等. 体外模拟猪胃条件下木聚糖酶活力稳定性研究［J］. 激光生物学报，2009，18(1)：87-90，100.
［11］李卫芬，孙建义.木聚糖酶的特性研究［J］. 浙江大学学报：农业与生命科学版，2001，27(1)：105-108.
［12］Cempírková R，Thér R. The effect of living conditions on selected indexes of raw cows milk［J］. Collection of Scientific Papers，Faculty of Agriculture in Cˇeské Budějovice，Series for Animal Sciences，2000，17(1)：55-71.
［13］Polizeli M L，Rizzatti A C，Monti R，et al. Xylanases from fungi：properties and industrial applications［J］. Applied Microbiology and Biotechnology，2005，67(5)：577-591.
［14］Colina A，Sulbarán-de-Ferrer B，Aiello C，et al. Xylanase production by Trichoderma reesei rut C-30 on rice straw［J］. Applied Biochemistry and Biotechnology，2003，108(1/2/3)：715-724.
［15］国春艳，刁其玉，乔宇，等. 酸性木聚糖酶产生菌株的筛选和酶学性质分析［J］. 中国农业科学，2010，43(7)：1524-1530.
［16］Wang S H，Chen J C. The protective effect of chitin and chitosan against Vibrio alginolyticus in white shrimp Litopenaeus vannamei［J］. Fish & Shellfish Immunology，2005，19(3)：191-204.
［17］Liang H Y，Wu Z H，Jian J C，et al. Protection of red snapper (Lutjanus sanguineus) against Vibrio alginolyticus with a DNA vaccine containing flagellin flaA gene［J］. Letters in Applied Microbiology，2011，52(2)：156-161.
［18］Jorge I，De la Rosa O，Navas-Cortés J A，et al. Extracellular xylanases from two pathogenic races of Fusarium oxysporum f. sp. ciceris：enzyme production in culture and purification and characterization of a major isoform as an alkaline endo-β-(1，4)-xylanase of low molecular weight［J］. Antonie van Leeuwenhoek，2005，88(1)：48-59.
［19］Miller G L. Use of dinitrosalicylic acid reagent for determination of reducing sugar［J］. Analytical Chemistry，1959，31(3)：426-428.
［20］Laemmli U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4［J］. Nature，1970，227(5259)：680-685.

相似文献/References:

[1]周杰,刘雅琴,龚洪兵,等.低温碱性脂肪酶产生菌的分离筛选及酶学性质[J].江苏农业科学,2013,41(04):325.
[2]常桂英,王俊玲,徐亚维,等.木聚糖酶xynB基因序列分析及表达载体构建[J].江苏农业科学,2013,41(10):31.
　Chang Guiying,et al.Sequence analysis and expression vector construction of xylanase xynB gene[J].Jiangsu Agricultural Sciences,2013,41(10):31.
[3]吴媚,徐凡.豆芽吡哆胺-丙酮酸转氨酶的分离纯化及部分酶学性质[J].江苏农业科学,2013,41(10):240.
　Wu Mei,et al.Purification and partial enzymatic properties of pyridoxamine-pyruvate transaminase in bean sprouts[J].Jiangsu Agricultural Sciences,2013,41(10):240.
[4]刘友勋,黄娟,闫明阳,等.活性电泳切胶法分离白腐菌(Trametes sp.B1)2种漆酶同工酶及其性质研究[J].江苏农业科学,2015,43(09):54.
　Liu Youxun,et al.Isolation of two laccase isoenzymes from Trametes sp. B1 by active gel electrophoresis and their enzymatic properties[J].Jiangsu Agricultural Sciences,2015,43(10):54.
[5]赵联正,谢占玲,赵朋.一种新的镰刀菌Q7-31木聚糖酶Xyn9的分离纯化鉴定及酶学特性[J].江苏农业科学,2015,43(05):42.
　Zhao Liamzheng,et al.Purification，identification and enzymatic characteristics of a new xylanase Xyn9 from Fusarium sp. Q7-31[J].Jiangsu Agricultural Sciences,2015,43(10):42.
[6]郄卫那,张兰英,何健,等.微生物产木聚糖酶研究进展[J].江苏农业科学,2014,42(07):387.
　Qie Weina,et al.Research progress on xylanase production by micro-organisms[J].Jiangsu Agricultural Sciences,2014,42(10):387.
[7]覃益民,张静茹,叶锦,等.康氏木霉内切β-葡聚糖苷酶的分离纯化及酶学性质[J].江苏农业科学,2015,43(01):40.
　Qin Yimin,et al.Purification and enzymology properties of Trichoderma koningii endo-β-glucanase[J].Jiangsu Agricultural Sciences,2015,43(10):40.
[8]王锐丽,刘敏杰,薛业敏.多功能融合酶基因Linker的优化及表达[J].江苏农业科学,2016,44(04):31.
　Wang Ruili,et al.Optimization and expression of multifunctional fusion enzyme gene linker[J].Jiangsu Agricultural Sciences,2016,44(10):31.
[9]刘蒲临,程德勇,缪礼鸿.产几丁质酶侧孢短芽孢杆菌M64的产酶条件优化及部分酶学性质研究[J].江苏农业科学,2016,44(10):468.
　Liu Pulin,et al.Study on enzyme production condition optimization and some enzymatic properties of chitinase-producing Brevibacillus laterosporu M64[J].Jiangsu Agricultural Sciences,2016,44(10):468.
[10]徐晓梅,薛双红,袁俊超,等.2株产低温蛋白酶耐冷菌株的筛选及酶学性质[J].江苏农业科学,2017,45(10):209.
　Xu Xiaomei,et al.Screening and enzymatic property of two low-temperature-protease-producing strains with cold tolerance[J].Jiangsu Agricultural Sciences,2017,45(10):209.

备注/Memo

备注/Memo:
收稿日期：2013-12-07
作者简介：郄卫那(1986—)，女，河北石家庄人，硕士研究生，主要研究方向为环境微生物与环境工程。E-mail：woniubeibao@gmail.com。
通信作者：贺芹，山东枣庄人，讲师，主要研究方向为发酵工程。E-mail：qhe@njau.edu.cn。
更新日期/Last Update: 2014-10-25