|本期目录/Table of Contents|

[1]龙宏燕,梁文英,陈风蔚,等.羊毛角蛋白降解菌的筛选鉴定及液体发酵条件优化[J].江苏农业科学,2021,49(4):200-204.
 Long Hongyan,et al.Screening and identification of wool keratin degrading bacteria and optimization of liquid fermentation conditions[J].Jiangsu Agricultural Sciences,2021,49(4):200-204.
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羊毛角蛋白降解菌的筛选鉴定及液体发酵条件优化(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第49卷
期数:
2021年第4期
页码:
200-204
栏目:
资源与环境
出版日期:
2021-02-20

文章信息/Info

Title:
Screening and identification of wool keratin degrading bacteria and optimization of liquid fermentation conditions
作者:
龙宏燕1梁文英2陈风蔚3王睿勇1
1.南京大学生命科学学院,江苏南京 210023; 2.南京大学金陵学院,江苏南京 210089;3.南京市淡水水产研究所,江苏南京 210017
Author(s):
Long Hongyanet al
关键词:
羊毛角蛋白液体发酵工艺优化降解菌筛选鉴定
Keywords:
-
分类号:
S182
DOI:
-
文献标志码:
A
摘要:
从长年堆积羊毛的土壤中筛选获得3株具有角蛋白降解功能的菌株,经形态观察和16S rRNA测序初步鉴定,并分别命名为链霉菌NJU-05、金黄杆菌NJU-07、地衣芽孢杆菌NJU-10。同时,进行液体发酵工艺优化,得到NJU-05菌株实验室水平的最佳液体发酵条件:温度为40 ℃,pH值为9.0,发酵周期为3 d;NJU-07菌株:温度为 35 ℃ ,pH值为9.0,发酵周期为4 d;NJU-10菌株:温度为35 ℃,pH值为9.0,发酵周期为4 d。
Abstract:
-

参考文献/References:

[1]Yin J,Rastogi S,Terry A E,et al. Self-organization of oligopeptides obtained on dissolution of feather keratins in superheated water[J]. Biomacromolecules,2007,8(3):800-806.
[2]张念荣,王全杰,张琦,等. 废弃羊毛角蛋白的资源化利用研究进展[J]. 西部皮革,2012,34(24):29-32.
[3]王慧玲,周彬. 再生羽毛蛋白/PVA共混长丝仿毛面料设计与生产[J]. 毛纺科技,2015,43(9):10-13.
[4]Wang L Y,Cheng G Y,Ren Y X,et al. Degradation of intact chicken feathers by Thermoactinomyces sp. CDF and characterization of its keratinolytic protease[J]. Applied Microbiology and Biotechnology,2015,99(9):3949-3959.
[5]Gupta R,Ramnani P. Microbial keratinases and their prospective applications:an overview[J]. Applied Microbiology and Biotechnology,2006,70(1):21-33.
[6]Gousterova A,Braikova D,Goshev I,et al. Degradation of keratin and collagen containing wastes by newly isolated thermoactinomycetes or by alkaline hydrolysis[J]. Letters in Applied Microbiology,2005,40(5):335-340.
[7]董荣斌,张玲,朱晓飞,等. 耐热链霉菌B221降解羽毛角蛋白的固体发酵条件研究[J]. 江苏农业科学,2007,35(6):249-251,267.
[8]Tomarelli R M,Charney J,Harding M L. The use of azoalbumin as a substrate in the colorimetric determination or peptic and tryptic activity[J]. The Journal of Laboratory and Clinical Medicine,1949,34(3):428-433.

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备注/Memo

备注/Memo:
收稿日期:2020-05-14
基金项目:国家自然科学基金(编号:30571354)。
作者简介:龙宏燕(1995— ),女,安徽淮南人,硕士研究生,主要从事应用微生物研究。E-mail:longhongyan1995@163.com。
通信作者:王睿勇,博士,副教授,主要从事应用微生物研究。 E-mail :wangry@nju.edu.cn。
更新日期/Last Update: 2021-02-20