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[1]王利红,张伟,陈圣源,等.苏姜猪SIRT7基因组织表达定位分析[J].江苏农业科学,2022,50(15):63-67.
　Wang Lihong,et al.Analysis of SIRT7 gene expression and localization in tissues of Sujiang Pig[J].Jiangsu Agricultural Sciences,2022,50(15):63-67.
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苏姜猪SIRT7基因组织表达定位分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第15期
页码:
63-67
栏目:
生物技术
出版日期:
2022-08-05

文章信息/Info

Title:
Analysis of SIRT7 gene expression and localization in tissues of Sujiang Pig
作者:
王利红1; 张伟1; 2; 陈圣源1; 朱军1
1.江苏农牧科技职业学院,江苏泰州 225300； 2.江苏姜曲海种猪场,江苏泰州 225300
Author(s):
Wang Lihong; et al
关键词:
苏姜猪; SIRT7; RT-PCR; ELISA; 免疫组化
Keywords:
-
分类号:
S828
DOI:
-
文献标志码:
A
摘要:
为深入分析去乙酰化转移酶Sirtuin 7(SIRT7)在苏姜猪不同脏器组织中的表达分布情况，采用实时荧光定量(RT-PCR)、酶联免疫吸附测定(ELISA)和免疫组织化学方法对苏姜猪11种脏器组织中SIRT7基因的表达分布进行研究。结果表明，SIRT7蛋白在所检测的组织样中均有表达，其中在胃和肠组织中表达量相对最高，显著高于其他组织(P<0.05)；SIRT7蛋白主要分布于胃腺、大肠和小肠的腺细胞中，在心脏、腿肌、背最长肌和腹脂中的表达量相对最低，显著低于其他组织中表达量(P<0.05)；在肺、肾和脾脏组织中，SIRT7蛋白主要分布于支气管上皮细胞、肺泡细胞、肾脏近远曲小管细胞、脾脏红髓区细胞；SIRT7基因在各脏器组织中mRNA和蛋白表达量间存在极显著正相关(P<0.01)。结果提示，SIRT7基因在苏姜猪组织中广泛表达，其转录和翻译水平存在极显著正相关，该基因功能主要与各脏器功能细胞的物质能量代谢和分泌功能有关。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2021-09-26
基金项目：江苏省泰州市科技支撑计划(农业)项目(编号：TN201612)；2021年江苏农牧科技职业学院大学生创新创业训练计划项目。
作者简介：王利红(1975—)，女，山西太原人，博士，教授，主要从事动物遗传育种与繁殖研究。E-mail：wanglihong345@126.com。
更新日期/Last Update: 2022-08-05