|本期目录/Table of Contents|

[1]郭绍雷,许建兰,张斌斌,等.基于广泛靶向代谢组的桃果实衰老软化过程差异代谢物筛选与鉴定[J].江苏农业科学,2024,52(6):198-205.
　Guo Shaolei,et al.Screening and identification of several metabolites associated with softening and senescence in peach fruit based on widely targeted metabolomics[J].Jiangsu Agricultural Sciences,2024,52(6):198-205.
点击复制

基于广泛靶向代谢组的桃果实衰老软化过程差异代谢物筛选与鉴定(PDF)
分享到：

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第52卷
期数:
2024年第6期
页码:
198-205
栏目:
贮藏加工与检测分析
出版日期:
2024-03-20

文章信息/Info

Title:
Screening and identification of several metabolites associated with softening and senescence in peach fruit based on widely targeted metabolomics
作者:
郭绍雷; 许建兰; 张斌斌; 张妤艳; 沈志军; 马瑞娟; 俞明亮
江苏省农业科学院果树研究所/江苏省高效园艺作物遗传改良重点实验室，江苏南京 210014
Author(s):
Guo Shaolei; et al
关键词:
桃果实; 衰老软化; 代谢组; 差异代谢物
Keywords:
-
分类号:
S662.101
DOI:
-
文献标志码:
A
摘要:
筛选与鉴定参与调控桃果实采后贮藏过程衰老软化相关代谢物质，以深入了解桃果实采后衰老软化相关生理生化机制。常温贮藏条件下，萘乙酸处理可加速霞晖8号七成熟桃果实衰老软化，采用超高液相色谱串联质谱技术，检测桃果实贮藏0、3、6 d代谢物质的变化，选取差异倍数值(≥2或≤0.5)，采用正交偏最小二乘判别分析模型所获得的变量重要性投影值(≥1)的代谢物为差异代谢物。结果表明，在霞晖8号桃果实5组样品中共检测到816种代谢物，可被分为11类，其中检测到酚酸类物质160种，占比最高。通过比较在各分组中代谢物定量信息的差异倍数变化前10的代谢物，结合KEGG信号通路的差异代谢物聚类分析，筛选并鉴定到可能参与桃果实软化的各种代谢物29种，其中脂质与酚酸类物质鉴定到最多，各有7种。
Abstract:
-

参考文献/References:

［1］Yoshioka H，Hayama H，Tatsuki M，et al. Cell wall modification during development of mealy texture in the stony-hard peach “Odoroki” treated with propylene［J］. Postharvest Biology and Technology，2010，55(1)：1-7.
［2］Brummell D A，Dal Cin V，Crisosto C H，et al. Cell wall metabolism during maturation，ripening and senescence of peach fruit［J］. Journal of Experimental Botany，2004，55(405)：2029-2039.
［3］Hayama H，Shimada T，Fujii H，et al. Ethylene-regulation of fruit softening and softening-related genes in peach［J］. Journal of Experimental Botany，2006，57(15)：4071-4077.
［4］Tucker G，Yin X R，Zhang A D，et al. Ethyleneand fruit softening［J］. Food Quality and Safety，2017，1(4)：253-267.
［5］Zhang Z Y，Wang N，Jiang S H，et al. Analysis of the xyloglucan endotransglucosylase/hydrolase gene family during apple fruit ripening and softening［J］. Journal of Agricultural and Food Chemistry，2017，65(2)：429-434.
［6］Iqbal N，Khan N A，Ferrante A，et al. Ethylene role in plant growth，development and senescence：interaction with other phytohormones［J］. Frontiers in Plant Science，2017，8：475.
［7］阚娟，刘俊，金昌海. 桃果实成熟软化与细胞壁降解相关糖苷酶及乙烯生物合成的关系［J］. 中国农业科学，2012，45(14)：2931-2938.
［8］徐小迪，李博强，秦国政，等. 果实采后品质维持的分子基础与调控技术研究进展［J］. 园艺学报，2020，47(8)：1595-1609.
［9］Xu H Y，Chen Y Y，Wang L B，et al. Transcriptome analysis reveals a regulation of ethylene-induced post-harvest senescence in pear fruit［J］. Scientia Horticulturae，2018，240：585-591.
［10］Qian M，Zhang Y K，Yan X Y，et al. Identification and expression analysis of polygalacturonase family members during peach fruit softening［J］. International Journal of Molecular Sciences，2016，17(11)：1933.
［11］Wang X B，Zeng W F，Ding Y F，et al. Peach ethylene response factor PpeERF2 represses the expression of ABA biosynthesis and cell wall degradation genes during fruit ripening［J］. Plant Science，2019，283：116-126.
［12］Smith D L，Abbott J A，Gross K C. Down-regulation of tomato beta-galactosidase 4 results in decreased fruit softening［J］. Plant Physiology，2002，129(4)：1755-1762.
［13］Guo S L，Song J，Zhang B B，et al. Genome-wide identification and expression analysis of beta-galactosidase family members during fruit softening of peach［Prunus persica (L.) Batsch］［J］. Postharvest Biology and Technology，2018，136：111-123.
［14］Liu H K，Qian M，Song C H，et al. Down-regulation of PpBGAL10 and PpBGAL16 delays fruit softening in peach by reducing polygalacturonase and pectin methylesterase activity［J］. Frontiers in Plant Science，2018，9：1015.
［15］Gu C，Guo Z H，Cheng H Y，et al. A HD-ZIP Ⅱ HOMEBOX transcription factor，PpHB.G7，mediates ethylene biosynthesis during fruit ripening in peach［J］. Plant Science，2019，278：12-19.
［16］Tadiello A，Ziosi V，Negri A S，et al. On the role of ethylene，auxin and a GOLVEN-like peptide hormone in the regulation of peach ripening［J］. BMC Plant Biology，2016，16：44.
［17］Wang X B，Pan L，Wang Y，et al. PpIAA1 and PpERF4 form a positive feedback loop to regulate peach fruit ripening by integrating auxin and ethylene signals［J］. Plant Science，2021，313：111084.
［18］Chen X M，Liu Y D，Zhang X，et al. PpARF6 acts as an integrator of auxin and ethylene signaling to promote fruit ripening in peach［J］. Horticulture Research，2023，10(9)：uhad158.
［19］Sawada Y，Akiyama K，Sakata A，et al. Widely targeted metabolomics based on large-scale MS/MS data for elucidating metabolite accumulation patterns in plants［J］. Plant & Cell Physiology，2009，50(1)：37-47.
［20］Chen W，Gong L，Guo Z L，et al. A novel integrated method for large-scale detection，identification，and quantification of widely targeted metabolites：application in the study of rice metabolomics［J］. Molecular Plant，2013，6(6)：1769-1780.
［21］闫乐乐，卜璐璐，牛良，等. 广泛靶向代谢组学解析桃蚜危害对桃树次生代谢产物的影响［J］. 中国农业科学，2022，55(6)：1149-1158.
［22］刘苏宁，王力荣，方伟超，等. 基于广泛靶向代谢组学的桃芽抗寒代谢物的筛选与鉴定［J］. 果树学报，2023，40(1)：1-12.
［23］生弘杰，卢素文，郑暄昂，等. 基于广泛靶向代谢组学的葡萄种子代谢物鉴定与比较分析［J］. 中国农业科学，2023，56(7)：1359-1376.
［24］石飞，邢玉青，李志刚，等. 基于广泛靶向代谢组学的冷藏冬枣成分分析［J］. 中国食品学报，2023，23(4)：324-333.
［25］Fan F Y，Huang C S，Tong Y L，et al. Widely targeted metabolomics analysis of white peony teas with different storage time and association with sensory attributes［J］. Food Chemistry，2021，362：130257.
［26］Tatsuki M，Nakajima N，Fujii H，et al. Increased levels of IAA are required for system 2 ethylene synthesis causing fruit softening in peach (Prunus persica L.Batsch)［J］. Journal of Experimental Botany，2013，64(4)：1049-1059.
［27］Fraga C G，Clowers B H，Moore R J，et al. Signature-discovery approach for sample matching of a nerve-agent precursor using liquid chromatography-mass spectrometry，XCMS，and chemometrics［J］. Analytical Chemistry，2010，82(10)：4165-4173.
［28］Thévenot E A，Roux A，Xu Y，et al. Analysis of the human adult urinary metabolome variations with age，body mass index，and gender by implementing a comprehensive workflow for univariate and OPLS statistical analyses［J］. Journal of Proteome Research，2015，14(8)：3322-3335.
［29］Ogata H，Goto S，Sato K，et al. KEGG：Kyoto encyclopedia of genes and genomes［J］. Nucleic Acids Research，1999，27(1)：29-34.
［30］蔡志翔，严娟，宿子文，等. 不同类型桃种质资源主要酚类物质含量评价［J］. 园艺学报，2022，49(5)：1008-1022.
［31］Liu H，Jiang W B，Cao J K，et al. Evaluation of antioxidant properties of extractable and nonextractable polyphenols in peel and flesh tissue of different peach varieties［J］. Journal of Food Processing and Preservation，2018，42(6)：e13624.
［32］卢娟芳，刘盛雨，芦旺，等. 不同类型桃果肉酚类物质及抗氧化活性分析［J］. 中国农业科学，2017，50(16)：3205-3214.
［33］朱辰晖，吴昊，施文卫，等. 对羟基肉桂酸(P-CA)处理对桃果实采后保鲜效果研究［J］. 食品科技，2022，47(5)：62-68.
［34］Xi W P，Zhang Y M，Sun Y J，et al. Phenolic composition of Chinese wild mandarin (Citrus reticulata Balnco.) pulps and their antioxidant properties［J］. Industrial Crops and Products，2014，52：466-474.
［35］Pan L，Zeng W F，Niu L，et al. PpYUC11，a strong candidate gene for the stony hard phenotype in peach (Prunus persica L.Batsch)，participates in IAA biosynthesis during fruit ripening［J］. Journal of Experimental Botany，2015，66(22)：7031-7044.
［36］汪开拓，雷长毅，韦盼盼，等. 亚精胺处理对桃果实贮藏品质及内源乙烯和多胺代谢的影响［J］. 食品与发酵工业，2020，46(10)：92-99，117.
［37］张海燕，饶景萍，戴斯琴，等. 外源腐胺对油桃采后生理及与其相关酶活性的影响［J］. 植物生理学通讯，2007，43(6)：1061-1064.
［38］曹永庆，冷平，潘烜，等. 脱落酸在桃果实成熟过程中的作用［J］. 园艺学报，2009，36(7)：1037-1042.
［39］Wang X B，Zeng W F，Ding Y F，et al. PpERF3 positively regulates ABA biosynthesis by activating PpNCED2/3 transcription during fruit ripening in peach［J］. Horticulture Research，2019，6：19.

相似文献/References:

[1]黎春红,周宏胜,张雷刚,等.适于桃果实货架保鲜的不同包装材料的筛选[J].江苏农业科学,2018,46(20):215.
　Li Chunhong,et al.Screening of packaging materials suitable for preservation of peach fruits during shelf-life[J].Jiangsu Agricultural Sciences,2018,46(6):215.
[2]邓红军,刘芳,杨明飞,等.采后桃果实耐冷性机理研究进展[J].江苏农业科学,2022,50(16):6.
　Deng Hongjun,et al.Research progress on cold tolerance mechanism of postharvest peach fruit[J].Jiangsu Agricultural Sciences,2022,50(6):6.

备注/Memo

备注/Memo:
收稿日期：2023-10-23
基金项目：现代农业产业技术体系建设专项(编号：CARS-30)；江苏省种业振兴“揭榜挂帅”项目(编号：JBGS［2021］082)。
作者简介：郭绍雷(1988—)，男，山东郯城人，博士，助理研究员，主要从事桃种质资源与新品种选育工作。E-mail：guoshaolei0305@126.com。
通信作者：俞明亮，博士，研究员，主要从事桃种质资源与新品种选育工作。E-mail：mly@jaas.ac.cn。
更新日期/Last Update: 2024-03-20