[1]乔新荣,张继英.茶树CsGPX基因克隆及干旱胁迫下的表达分析[J].江苏农业科学,2018,46(08):42-44.
　Qiao Xinrong,et al.Cloning of CsGPX gene in Camellia sinensis and its expression analysis under drought stress[J].Jiangsu Agricultural Sciences,2018,46(08):42-44.
点击复制

茶树CsGPX基因克隆及干旱胁迫下的表达分析()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第46卷
期数:
2018年08期
页码:
42-44
栏目:
生物技术
出版日期:
2018-04-20

文章信息/Info

Title:
Cloning of CsGPX gene in Camellia sinensis and its expression analysis under drought stress
作者:
乔新荣;  张继英
信阳农林学院，河南信阳 464000
Author(s):
Qiao Xinrong; et al
关键词:
茶树; GPX基因; RT-PCR技术; 干旱胁迫
Keywords:
-
分类号:
Q344+.13；S571.101
DOI:
-
文献标志码:
A
摘要:
采用反转录-PCR(reverse transcription-polymerase chain reaction，简称RT-PCR)技术，从茶树叶片中克隆长387 bp的CsGPX基因片段，生物信息学分析结果表明该基因片段编码的氨基酸序列为GPX蛋白的保守序列，系统进化树分析结果揭示该蛋白与拟南芥AtGPX6、杨树PtGXP6等亲缘关系较近。定量PCR分析结果表明，干旱胁迫处理12 h内，GPX表达量明显上升，初步分析茶树CsGPX基因在干旱胁迫反应中发挥重要作用。
Abstract:
-

参考文献/References:

［1］Mittler R. Oxidative stress，antioxidants and stress tolerance［J］. Trends in Plant Science，2002，7(9)：405-410.
［2］Yang X D，Li W J，Liu J Y. Isolation and characterization of a novel PHGPx gene in Raphanus sativus［J］. Biochimica et Biophysica Acta，2005，1728(3)：199-205.
［3］Rodriguez Milla M A，Maurer A，Rodriguez Huete A，et al. Glutathione peroxidase genes in Arabidopsis are ubiquitous and regulated by abiotic stresses through diverse signaling pathways［J］. Plant Journal，2003，36(5)：602-615.
［4］Gaber A，Ogata T，Maruta T，et al. The involvement of Arabidopsis glutathione peroxidase 8 in the suppression of oxidative damage in the nucleus and cytosol［J］. Plant & Cell Physiology，2012，53(9)：1596-1606.
［5］肖蓉，罗慧珍，张小娟，等. 干旱和盐胁迫条件下枣树谷胱甘肽过氧化物酶基因(ZjGPX)的差异表达及功能分析［J］. 中国农业科学，2015，48(14)：2806-2817.
［6］Sugimoto M，Sakamoto W. Putative phospholipid hydroperoxide glutathione peroxidase gene from Arabidopsis thaliana induced by oxidative stress［J］. Genes & Genetic Systems，1997，72(5)：311-316.
［7］Zhai C Z，Zhao L，Yin L J，et al. Two wheat glutathione peroxidase genes whose products are located in chloroplasts improve salt and H2O2 tolerances in Arabidopsis［J］. PLoS One，2013，8(10)：e73989.
［8］Goswami A，Banerjee R，Raha S. Drought resistance in rice seedlings conferred by seed priming：role of the anti-oxidant defense mechanisms［J］. Protoplasma，2013，250(5)：1115-1129.

相似文献/References:

[1]李金,魏艳丽,庞磊,等.茶树咖啡碱合成途径中TCS1、TIDH、SAMS的基因表达量差异及其与咖啡碱含量的相关性[J].江苏农业科学,2013,41(10):21.
　Li Jin,et al.Differences in expression of TCS1、TIDH and SAMS genes in caffeine synthetic route of Camellia Sinensis and their correlation with caffeine contents[J].Jiangsu Agricultural Sciences,2013,41(08):21.
[2]朱韦京,余树全,汪赛,等.不同酸雨作用方式对茶树幼苗生长与光合特征参数的影响[J].江苏农业科学,2014,42(10):232.
　Zhu Weijing,et al.Effects of different acid rain action modes on growth and photosynthetic parameters of Camellia sinensis seedlings[J].Jiangsu Agricultural Sciences,2014,42(08):232.
[3]李荣林,李珍珍,杨亦扬,等.以诱导抗性为基础的茶树病虫害控制新技术[J].江苏农业科学,2013,41(11):145.
　Li Ronglin,et al.New diseases and insect pests control techniques for tea tree based on induced resistance[J].Jiangsu Agricultural Sciences,2013,41(08):145.
[4]王雪萍,龚自明,高士伟,等.ABT1号生根粉对茶树穴盘扦插生根的影响[J].江苏农业科学,2013,41(11):277.
　Wang Xueping,et al.Effect of rooting powder ABT1 on rooting of tea tree plug seedlings[J].Jiangsu Agricultural Sciences,2013,41(08):277.
[5]周萌,李友勇,孙雪梅,等.基于EST-SSR标记的云南野生茶树遗传多样性分析[J].江苏农业科学,2013,41(12):22.
　Zhou Meng,et al.Genetic diversity analysis of wild tea trees in Yunnan Province based on EST-SSR markers[J].Jiangsu Agricultural Sciences,2013,41(08):22.
[6]杨亦扬,胡雲飞,李荣林,等.不同茶树品种的碧螺春茶适制性[J].江苏农业科学,2015,43(09):219.
　Yang Yiyang,et al.Study on processing suitability of Biluochun tea from different tea plant varieties[J].Jiangsu Agricultural Sciences,2015,43(08):219.
[7]胡雲飞,杨亦扬,李荣林,等.不同时段喷施叶面肥对春茶新梢生长与品质的影响[J].江苏农业科学,2015,43(07):170.
　Hu Yunfei,et al.Effects of sparying foliage fertilizer at different times on growth and quality of fresh tea new shoots[J].Jiangsu Agricultural Sciences,2015,43(08):170.
[8]李荣林,杨亦扬,胡雲飞,等.茶树的抗虫性和抗性育种研究[J].江苏农业科学,2015,43(05):1.
　Li Rongling,et al.Study on insect resistance and stress-resistance breeding of tea plant[J].Jiangsu Agricultural Sciences,2015,43(08):1.
[9]王海斌,叶江华,孔祥海,等.铜胁迫下不同茶树的生理响应及亚细胞水平铜分布特性[J].江苏农业科学,2016,44(11):219.
　Wang Haibin,et al.Physiological response and copper distribution characteristics in subcellular level of different tea tree under copper stress[J].Jiangsu Agricultural Sciences,2016,44(08):219.
[10]田甜,韦锦坚,陈远权,等.茶树的铝、硒、钙营养及互作研究综述[J].江苏农业科学,2016,44(12):29.
　Tian Tian,et al.On nutrition and interaction of aluminum， selenium and calcium in tea plant：a review[J].Jiangsu Agricultural Sciences,2016,44(08):29.

备注/Memo

备注/Memo:
收稿日期：2016-11-06
基金项目：河南省教育厅重点科研项目(编号：15A180058)。
作者简介：乔新荣(1972—)，女，河南新密人，博士，副教授，主要从事植物生理及分子生物学研究。Tel：(0376)6698023；E-mail：xinrong806@163.com。
更新日期/Last Update: 2018-04-20