|本期目录/Table of Contents|

[1]孙明慧,刘丹丹,吴琼,等.茶树BURP基因家族全基因组鉴定与表达分析[J].江苏农业科学,2025,53(4):242-253.
　Sun Minghui,et al.Whole-genome identification and expression analysis of BURP gene family in tea plants[J].Jiangsu Agricultural Sciences,2025,53(4):242-253.
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茶树BURP基因家族全基因组鉴定与表达分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第4期
页码:
242-253
栏目:
多抗性基因
出版日期:
2025-02-20

文章信息/Info

Title:
Whole-genome identification and expression analysis of BURP gene family in tea plants
作者:
孙明慧; 刘丹丹; 吴琼; 焦小雨; 王文杰
安徽省农业科学院茶叶研究所，安徽合肥 230001
Author(s):
Sun Minghui; et al
关键词:
BURP基因家族; 茶树; 基因表达; 非生物胁迫; 全基因组鉴定
Keywords:
-
分类号:
S188；S571.101
DOI:
-
文献标志码:
A
摘要:
茶［Camellia sinensis(L.) Kuntze］因其极高的营养价值和经济重要性而在全球范围内种植。为了研究茶树中BURP的作用，基于茶树全基因组对茶树中BURP基因家族进行筛选和鉴定。通过TBtools查找筛选包含有PF03181结构域的序列，使用生物信息学对其编码的蛋白质进行染色体定位、系统进化关系、蛋白质理化性质和共线性关系等分析。基于转录组数据挑选对胁迫响应较强烈的基因4个，即CsBURP14、CsBURP7、CsBURP10和CsBURP15，利用实时荧光定量聚合酶链式反应(real-time fluorescent quantitative PCR，qPCR)方法检测其在冷、盐和干旱胁迫下的表达情况。利用茶树基因组数据鉴定出17个BURP基因，聚类为3个亚组，其中PG1β-like包含5条，BNM2-like包含8条，RD22-like包含4条。结构域预测发现只有11条基因包含完整的BURP结构域，6条序列只包含其中的1或2个motif，而CsBURP1不包含这3个motif。CsBURP14、CsBURP7、CsBURP10和CsBURP15均响应干旱胁迫、冷胁迫和盐胁迫。CsBURP7响应最剧烈，在低温处理下为负调控，保持在初始表达量的1/3左右，干旱处理则为正调控，12 h时为初始值的6倍。CsBURP家族对低温胁迫、盐胁迫和干旱胁迫均有响应，推测其在茶树抗逆中发挥重要作用。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2024-10-29
基金项目：国家自然科学基金联合基金(编号：U23A20213)；安徽省茶叶产业技术体系项目(编号：AHCYJSTX-11)；安徽省农业科学院成果转化项目(编号：2024YL041)。
作者简介：孙明慧(1994—)，女，河南睢县人，硕士，助理研究员，主要从事茶树种质资源与遗传育种研究。E-mail：sunminghui@aaas.org.cn。
通信作者：王文杰，研究员，主要从事茶树新品种选育、茶叶加工与品质研究。E-mail：391590137@qq.com。
更新日期/Last Update: 2025-02-20