[1]何浩雯,彭梦林,张晋豪,等.解淀粉芽孢杆菌Y67的筛选鉴定及对草莓角斑病的生防效果[J].江苏农业科学,2025,53(13):171-179.
　He Haowen,et al.Screening and identification of Bacillus amyloliquefaciens Y67 and its biocontrol effect on angular leaf spot of strawberry[J].Jiangsu Agricultural Sciences,2025,53(13):171-179.
点击复制

解淀粉芽孢杆菌Y67的筛选鉴定及对草莓角斑病的生防效果()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第53卷
期数:
2025年第13期
页码:
171-179
栏目:
植物保护
出版日期:
2025-07-05

文章信息/Info

Title:
Screening and identification of Bacillus amyloliquefaciens Y67 and its biocontrol effect on angular leaf spot of strawberry
作者:
何浩雯1; 彭梦林1; 张晋豪1; 王菊芬3; 魏兰芳1; 2; 姬广海1
1.云南农业大学省部共建云南生物资源保护与利用国家重点实验室，云南昆明 650201；2.云南农业大学农科专业基础实验教学中心，云南昆明 650201； 3.云南省芒市种子管理站，云南芒市 678400
Author(s):
He Haowen; et al
关键词:
解淀粉芽孢杆菌; 草莓细菌性角斑病; 防效; 生物防治; 稳定性
Keywords:
-
分类号:
S436.68+4；S182
DOI:
-
文献标志码:
A
摘要:
近年来，草莓角斑病的发生严重影响草莓产量和品质，造成严重的经济损失。分离筛选防治草莓角斑病的生防细菌日益重要。从草莓健康根际土中分离鉴定1株对草莓角斑病有拮抗作用的细菌，采用牛津杯法检测菌株发酵液的广谱抑菌活性及温度、pH值、紫外线和蛋白酶对其抑菌活性稳定性的影响；酸沉淀法、硫酸铵沉淀法、大孔树脂吸附和乙酸乙酯萃取法初步提取菌株发酵液抑菌粗提物，根据脂肽类和聚酮类化合物合成相关基因序列对拮抗菌株进行扩增检测与活性检测。结果表明，筛选到1株具有良好拮抗效果的解淀粉芽孢杆菌(Bacillus amyloliquefaciens)Y6-7；抑菌谱测定表明，菌株Y6-7具有广谱的抑菌活性；菌株Y6-7的生物学特性研究表明，该菌最适生长温度为 30 ℃，具有较好的盐耐受性和耐酸碱性；Y6-7菌株具有聚酮类物质和脂肽类物质相关合成基因，草莓角斑病菌YM2的活性抑菌物质具有良好的热稳定性和紫外线稳定性，耐酸碱，对溶菌酶和蛋白酶K敏感；防治草莓角斑病盆栽试验结果表明，Y6-7菌株对草莓角斑病防效达到80.16%。说明筛选出的解淀粉芽孢杆菌Y6-7为草莓角斑病的生物防治提供了防效好且稳定的菌种，在农业防治中生防前景良好，具有巨大的生防应用价值。
Abstract:
-

参考文献/References:

［1］Kennedy B W，King T H. Angular leaf spot of strawberry caused by Xanthomonas fragariae sp. nov［J］. Phytopathology，1962，52(9)：873-875.
［2］Lynch K V，Tremblay R J A. Control of angular leaf spot (Xanthomonas fragariae) in strawberry using tribasic copper sulphate［J］. Advanced Strawberry Research，1996，15(18)：20-25.
［3］Lamichhane J R. Xanthomonas arboricola diseases of stone fruit，almond，and walnut trees：progress toward understanding and management［J］. Plant Disease，2014，98(12)：1600-1610.
［4］Miller M，Deiulio A，Holland C，et al. Complete genome sequence of Xanthomonas phage RiverRider，a novel N4-like bacteriophage that infects the strawberry pathogen Xanthomonas fragariae［J］. Archives of Virology，2020，165(6)：1481-1484.
［5］Daranas N，Roselló G，Cabrefiga J，et al. Biological control of bacterial plant diseases with Lactobacillus plantarum strains selected for their broad-spectrum activity［J］. Annals of Applied Biology，2019，174(1)：92-105.
［6］起登凤，邹良平，周登博，等. GA1-2菌株的分离鉴定及其对香蕉尖孢镰刀菌的抑菌效果［J］. 植物保护学报，2017，44(5)：809-816.
［7］Brunner K，Zeilinger S，Ciliento R，et al. Improvement of the fungal biocontrol agent Trichoderma atroviride to enhance both antagonism and induction of plant systemic disease resistance［J］. Applied and Environmental Microbiology，2005，71(7)：3959-3965.
［8］戚菊峰.铁皮石斛茎腐病病原菌鉴定及Bacillus velezensis D-12的防病促生研究［D］. 杭州：浙江大学，2021.
［9］Braun P G，Hildebrand P D.Effect of sugar alcohols，antioxidants and activators of systemically acquired resistance on severity of bacterial angular leaf spot (Xanthomonas fragariae) of strawberry in controlled environment conditions［J］. Canadian Journal of Plant Pathology，2013，35(1)：20-26.
［10］贾熙超，杨淑珂，孙红炜，等. 棉花枯萎病生防菌的筛选及其抑菌机理初探［J］. 山东农业科学，2021，53(2)：94-101，105.
［11］桑建伟，杨扬，陈奕鹏，等. 内生解淀粉芽孢杆菌BEB17脂肽类和聚酮类化合物的抑菌活性分析［J］. 植物病理学报，2018，48(3)：402-412.
［12］Roberts P D，Berger R D，Jones J B，et al. Disease progress，yield loss，and control of Xanthomonas fragariae on strawberry plants［J］. Plant Disease，1997，81(8)：917-921.
［13］Compant S，Duffy B，Nowak J，et al. Use of plant growth-promoting bacteria for biocontrol of plant diseases：principles，mechanisms of action，and future prospects［J］. Applied and Environmental Microbiology，2005，71(9)：4951-4959.
［14］Sylla J，Alsanius B W，Krüger E，et al. Control of Botrytis cinerea in strawberries by biological control agents applied as single or combined treatments［J］. European Journal of Plant Pathology，2015，143(3)：461-471.
［15］Pertot I，Zasso R，Amsalem L，et al. Integrating biocontrol agents in strawberry powdery mildew control strategies in high tunnel growing systems［J］. Crop Protection，2008，27(3/4/5)：622-631.
［16］Mishra S，Arora N K. Evaluation of rhizospheric Pseudomonas and Bacillus as biocontrol tool for Xanthomonas campestris pv. campestris［J］. World Journal of Microbiology & Biotechnology，2012，28(2)：693-702.
［17］Naue C，Rocha D，Moura A. Biological control of tomato bacterial spot by seed microbiolization［J］. Tropical Plant Pathology，2014，39(5)：413-416.
［18］van Hop D，Phuong Hoa P T，Quang N D，et al. Biological control of Xanthomonas oryzae pv. oryzae causing rice bacterial blight disease by Streptomyces toxytricini VN08-A-12，isolated from soil and leaf-litter samples in Vietnam［J］. Biocontrol Science，2014，19(3)：103-111.
［19］Chen M C，Wang J P，Zhu Y J，et al. Antibacterial activity against Ralstonia solanacearum of the lipopeptides secreted from the Bacillus amyloliquefaciens strain FJAT-2349［J］. Journal of Applied Microbiology，2019，126(5)：1519-1529.
［20］Solanki M K，Singh R K，Srivastava S，et al. Characterization of antagonistic-potential of two Bacillus strains and their biocontrol activity against Rhizoctonia solani in tomato［J］. Journal of Basic Microbiology，2015，55(1)：82-90.
［21］Stein T. Bacillus subtilis antibiotics：structures，syntheses and specific functions［J］. Molecular Microbiology，2005，56(4)：845-857.
［22］Santiago C D，Yagi S，Ijima M，et al. Bacterial compatibility in combined inoculations enhances the growth of potato seedlings［J］. Microbes and Environments，2017，32(1)：14-23.
［23］May A，Coelho L F，Pedrinho A，et al. The use of indigenous bacterial community as inoculant for plant growth promotion in soybean cultivation［J］. Archives of Agronomy and Soil Science，2023，69(1)：135-150.
［24］杨洪凤，余向阳，薛雅蓉，等. 内生解淀粉芽孢杆菌CC09在小麦根部定殖的电镜观察及防病效果［J］. 中国生物防治学报，2014，30(6)：839-844.
［25］黎起秦，罗宽，林纬，等. 内生菌B47的定殖能力及其对番茄青枯病的防治作用［J］. 植物保护学报，2006，33(4)：363-368.
［26］刘爽，唐锐，谭丹，等. 解淀粉芽孢杆菌OMR1-7菌株对拟石莲花属多肉植物黑腐病的生防效果［J］. 云南农业大学学报(自然科学)，2024，39(1)：54-63.

相似文献/References:

[1]张荣胜,戴秀华,陈志谊.解淀粉芽孢杆菌Lx-11对水稻细菌性条斑病的防治效果[J].江苏农业科学,2014,42(10):115.
　Zhang Rongsheng,et al.Control effect of Bacillus amyloliquefaciens Lx-11 on rice bacterial leaf streak[J].Jiangsu Agricultural Sciences,2014,42(13):115.
[2]屈俊廷,金海强,沈国娟,等.人参锈腐病生防用解淀粉芽孢杆菌Y-S-Y12菌株发酵条件的优化[J].江苏农业科学,2016,44(03):158.
　Qu Junting,et al.Optimization of fermentation conditions of Bacillus amyloliquefaciens srain Y-S-Y12 for biocontrol of ginseng rust rot[J].Jiangsu Agricultural Sciences,2016,44(13):158.
[3]王超,李宏伟,王翠,等.防治大白菜软腐病细菌菌株的筛选与鉴定[J].江苏农业科学,2013,41(12):114.
　Wang Chao,et al.Screening and identification of antagonistic bacteria controlling Chinese cabbage soft rot[J].Jiangsu Agricultural Sciences,2013,41(13):114.
[4]孙梅,张维娜,高亮,等.解淀粉芽孢杆菌JSSW-LA的分离鉴定及对病原菌拮抗特性[J].江苏农业科学,2016,44(04):275.
　Sun Mei,et al.Isolation， identification and pathogen antagonist properties of Bacillus amyloliquefaciens JSSW-LA[J].Jiangsu Agricultural Sciences,2016,44(13):275.
[5]朱弘元,康健,范昕,等.解淀粉芽孢杆菌B15产脂肽的分离鉴定及抑菌机理[J].江苏农业科学,2016,44(05):186.
　Zhu Hongyuan,et al.Identification and preliminary study on antifungal activity of lipopeptide produced by bacillus amyloliquefaciens B15[J].Jiangsu Agricultural Sciences,2016,44(13):186.
[6]裘纪莹,陈相艳,吴发萍,等.解淀粉芽孢杆菌NCPSJ7菌株高产抗菌物质的发酵条件优化[J].江苏农业科学,2016,44(01):311.
　Qiu Jiying,et al.Optimization of high production fermentation conditions of antimicrobial substance by Bacillus amyloliquefaciens strain NCPSJ7[J].Jiangsu Agricultural Sciences,2016,44(13):311.
[7]高同国,姜军坡,郭晓军,等.马铃薯疮痂病高效拮抗菌的筛选及鉴定[J].江苏农业科学,2016,44(12):157.
　Gao Tongguo,et al.Screening and identification of antagonistic bacteria against Streptomyces scabies on potato scab[J].Jiangsu Agricultural Sciences,2016,44(13):157.
[8]郭政宏,周彪,严亨秀.藏猪源解淀粉芽孢杆菌的益生性、安全性和应用性研究[J].江苏农业科学,2017,45(14):137.
　Guo Zhenghong,et al.Study on probiotic function，safety and application of Bacillus amyloliquefaciens from Tibetan pigs[J].Jiangsu Agricultural Sciences,2017,45(13):137.
[9]魏蜜,张伟,管维轩,等.1株油茶真菌病害拮抗菌株的筛选鉴定及其拮抗作用[J].江苏农业科学,2017,45(18):100.
　Wei Mi,et al.Screening and identification of an antagonistic strain of Camellia oleifera fungi disease and its antagonistic effect[J].Jiangsu Agricultural Sciences,2017,45(13):100.
[10]汪晶晶,曹雪梅,李欢,等.抗菌海洋解淀粉芽孢杆菌GM-1-2菌株摇瓶发酵培养基和发酵条件优化[J].江苏农业科学,2018,46(08):291.
　Wang Jingjing,et al.Optimization of medium components and cultural conditions of marine Bacillus amyloliquefaciens GM-1-2 strain with antifungal activity[J].Jiangsu Agricultural Sciences,2018,46(13):291.

备注/Memo

备注/Memo:
收稿日期：2024-06-29
基金项目：云南省科技厅基础研究专项-重点项目(编号：202201AS070313)。
作者简介：何浩雯(2000—)，女，四川内江人，硕士研究生，研究方向为植物病害生物防治研究。E-mail：2501787952@qq.com。
通信作者：姬广海，博士，教授，从事植物病害生物防治研究。E-mail：jghai001@163.com。
更新日期/Last Update: 2025-07-05