[1]陈欣怡,张洋,于单,等.黑曲霉发酵藤茶对酿酒酵母抗逆性能的影响及其机制研究[J].江苏农业科学,2026,54(7):186-194.
　Chen Xinyi,et al.Effect of vine tea fermented with Aspergillus niger on stress resistance of Saccharomyces cerevisiae and its mechanism[J].Jiangsu Agricultural Sciences,2026,54(7):186-194.
点击复制

黑曲霉发酵藤茶对酿酒酵母抗逆性能的影响及其机制研究()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第54卷
期数:
2026年第7期
页码:
186-194
栏目:
贮藏加工与检测分析
出版日期:
2026-04-05

文章信息/Info

Title:
Effect of vine tea fermented with Aspergillus niger on stress resistance of Saccharomyces cerevisiae and its mechanism
作者:
陈欣怡1; 张洋2; 于单2; 缪程平2; 徐涛1; 李加友2
1.浙江理工大学生命科学与医药学院，浙江杭州 310018； 2.嘉兴大学生物与化学工程学院，浙江嘉兴 314001
Author(s):
Chen Xinyi; et al
关键词:
黑曲霉; 藤茶; 酿酒酵母; 抗逆性能; 非靶向代谢组学
Keywords:
-
分类号:
S188+.4；TS201.2
DOI:
-
文献标志码:
A
摘要:
酿酒酵母是一种重要且应用广泛的工业微生物，提升其抗逆性能可以更好地发挥其产业价值。利用黑曲霉对藤茶进行发酵处理，进一步考察提取物对酿酒酵母环境胁迫耐受性的影响，通过 UPLC-QTOF-MS技术结合非靶向代谢组学对胁迫耐受性的作用机理进行分析。结果表明，黑曲霉发酵能将藤茶总黄酮含量明显提升至184.13±056 mg/g，较未发酵组增加18.86%；同时促使黄酮类化合物发生甲基化、脱羟基化等生物转化，其中，原花青素A1、朝藿素B、二氢杨梅素等3种化合物含量下调，5，7，3′，4′，5′-五甲氧基黄酮、伞房花耳草素、异鼠李素等7种黄酮化合物含量明显上调。胁迫试验结果显示，发酵藤茶提取物(0.5 mg/mL)可明显增强酿酒酵母对乙醇(10%浓度)、高温(41 ℃)、酸度(pH值3)、高糖(350 g/L葡萄糖)的耐受性，活细胞数分别较对照组提升123%、33.67%、26.04%、4077%。机制分析表明，甲基化黄酮通过改善脂溶性和膜通透性促进酵母摄取，增强抗氧化应激能力(如清除ROS)，并通过上调热休克蛋白(HSP)基因表达而提升热耐受性。本研究揭示了黑曲霉发酵藤茶通过优化黄酮组分提升酿酒酵母抗逆性能的分子机制，可为开发新型酵母胁迫保护剂提供理论依据。
Abstract:
-

参考文献/References:

［1］Bordet F，Romanet R，Bahut F，et al. Impact of Saccharomyces cerevisiae yeast inoculation mode on wine composition［J］. Food Chemistry，2024，441：138391.
［2］Wu D H，Chen Z Q，Lv Y X，et al. Coculturing of non-Saccharomyces cerevisiae and Saccharomyces cerevisiae for improving the aroma quality and antioxidant characteristics of beer with Yuanan yellow tea［J］. Food Bioscience，2024，58：103725.
［3］Svedlund N，Kiepert A，Rodríguez Pérez J，et al. Enhancement of dry-hopped cider aroma through selection of apple cultivar，hop variety and yeast strain［J］. Food Microbiology，2025，129：104755.
［4］Wu X X，Wan X W，Yu H B，et al. Recent advances in CRISPR-Cas system for Saccharomyces cerevisiae engineering［J］. Biotechnology Advances，2025，81：108557.
［5］Walker G M，Basso T O. Mitigating stress in industrial yeasts［J］. Fungal Biology，2020，124(5)：387-397.
［6］Zeng T X，Song Y J，Qi S Y，et al. A comprehensive review of vine tea：Origin，research on Materia Medica，phytochemistry and pharmacology［J］. Journal of Ethnopharmacology，2023，317：116788.
［7］Luo Q H，Qiu J，Chen M X，et al. Vine tea (Ampelopsis grossedentata) ameliorates chronic alcohol-induced hepatic steatosis，oxidative stress，and inflammation via YTHDF2/PGC-1α/SIRT3 axis［J］. Food Research International，2025，209：116321.
［8］Chen J N，Wang X T，Xia T，et al. Molecular mechanisms and therapeutic implications of dihydromyricetin in liver disease［J］. Biomedicine & Pharmacotherapy，2021，142：111927.
［9］Chen Y H，Cheng L，Zhang X，et al. Transcriptomic and proteomic effects of (-)-epigallocatechin 3-O-(3-O-methyl) gallate (EGCG3″Me) treatment on ethanol-stressed Saccharomyces cerevisiae cells［J］. Food Research International，2019，119：67-75.
［10］Sun C C，Li Y，Yin Z P，et al. Physicochemical properties of dihydromyricetin and the effects of ascorbic acid on its stability and bioavailability［J］. Journal of the Science of Food and Agriculture，2021，101(9)：3862-3869.
［11］Parshikov I A，Sutherland J B.Biotransformation of steroids and flavonoids by cultures of Aspergillus niger［J］. Applied Biochemistry and Biotechnology，2015，176(3)：903-923.
［12］袁桥玉，刘新桥. 藤茶中总黄酮的含量测定［J］. 内蒙古中医药，2014，33(30)：126-127.
［13］Kasavi C，Eraslan S，Oner E T，et al. An integrative analysis of transcriptomic response of ethanol tolerant strains to ethanol in Saccharomyces cerevisiae［J］. Molecular BioSystems，2016，12(2)：464-476.
［14］Ndukwe J K，Aliyu G O，Onwosi C O，et al. Mechanisms of weak acid-induced stress tolerance in yeasts：Prospects for improved bioethanol production from lignocellulosic biomass［J］. Process Biochemistry，2020，90：118-130.
［15］Hoshida H，Akada R. High-temperature bioethanol fermentation by conventional and nonconventional yeasts［M］//Biotechnology of yeasts and filamentous fungi. Cham：Springer International Publishing，2017.
［16］Morano K A，Grant C M，Scott Moye-Rowley W. The response to heat shock and oxidative stress in Saccharomyces cerevisiae［J］. Genetics，2012，190(4)：1157-1195.
［17］Wen L R，Jiang Y M，Yang J L，et al. Structure，bioactivity，and synthesis of methylated flavonoids［J］. Annals of the New York Academy of Sciences，2017，1398(1)：120-129.
［18］Warnsmann V，Hainbuch S，Osiewacz H D. Quercetin-induced lifespan extension in Podospora anserina requires methylation of the flavonoid by the O-methyltransferase PaMTH1［J］. Frontiers in Genetics，2018，9：160.
［19］Büchter C，Ackermann D，Honnen S，et al. Methylated derivatives of myricetin enhance life span in Caenorhabditis elegans dependent on the transcription factor DAF-16［J］. Food & Function，2015，6(10)：3383-3392.
［20］Liu C Y，Sun Y Y，Wang S Q，et al. Dihydromyricetin from Ampelopsis grossedentata and its derivatives：structural characterization and anti-hepatocellular carcinoma activity［J］. Journal of Molecular Structure，2022，1258：132677.
［21］Wang Z Y，Wang X S，Guo Z，et al. Reduning attenuates LPS-induced human unmilical vein endothelial cells (HUVECs) apoptosis through PI3K-AKT signaling pathway［J］. Frontiers in Pharmacology，2022，13：921337.
［22］Xie Y C，Gong S C，Wang L K，et al. Unraveling the treatment effects of Huanglian Jiedu decoction on drug-induced liver injury based on network pharmacology，molecular docking and experimental validation［J］. BMC Complementary Medicine and Therapies，2024，24(1)：219.
［23］Yu R，Zhang Y P，Wang T，et al. Effect of Tricin on cardiomyocyte damage caused by diabetic cardiomyopathy (DCM)［J］. BMC Cardiovascular Disorders，2024，24(1)：668.
［24］Yang X Y，Wang H W，Shen C F，et al. Effects of isorhamnetin on liver injury in heat stroke-affected rats under dry-heat environments via oxidative stress and inflammatory response［J］. Scientific Reports，2024，14(1)：7476.

相似文献/References:

[1]郭博恺,李祝,万科,等.黑曲霉不同极性分离产物的抗真菌及抗氧化能力[J].江苏农业科学,2016,44(01):290.
　Guo Bokai,et al.Antifungal and antioxidant activity of different polarity components from Aspergillus niger[J].Jiangsu Agricultural Sciences,2016,44(7):290.
[2]申丽,李晓雯,朱力.狗牙根内生黑曲霉(Aspergillus niger)的化学成分研究[J].江苏农业科学,2015,43(12):422.
　Shen Li,et al.Study on chemical constituents from endophyte Aspergillus niger in Cynodon dactylon[J].Jiangsu Agricultural Sciences,2015,43(7):422.
[3]平康康,路 飞,王泽键,等.基于在线生理参数的黑曲霉生产葡萄糖酸钠发酵动力学模型[J].江苏农业科学,2015,43(07):375.
　Ping Kangkang,et al.Kinetic models of sodium gluconate fermentation by Aspergillus niger based on on-line physiological parameters[J].Jiangsu Agricultural Sciences,2015,43(7):375.
[4]姜春阳,贾春云,张丽芳,等.微生物胞外聚合物对土壤中芘降解效果的促进作用[J].江苏农业科学,2015,43(06):303.
　Jiang Chunyang,et al.Auxo-action of microorganisms extracellular polymers to degradation of pyrene in soil[J].Jiangsu Agricultural Sciences,2015,43(7):303.
[5]熊进,何顺荣,吴鑫颖,等.黑曲霉液态发酵制备没食子酸的工艺研究[J].江苏农业科学,2015,43(05):250.
　Xiong Jin,et al.Study on production process of gallic acid by submerged fermentation of Aspergillus niger[J].Jiangsu Agricultural Sciences,2015,43(7):250.
[6]付明,李胜华,张婷,等.RP-HPLC法测定湘西藤茶二氢杨梅素的含量[J].江苏农业科学,2014,42(07):332.
　Fu Ming,et al.Determination of dihydromyricetin contents in Ampelopsis grossedentata by RP-HPLC[J].Jiangsu Agricultural Sciences,2014,42(7):332.
[7]杜国军,田英华,刘晓兰.果胶酶高产菌株的微波-硫酸二乙酯复合诱变选育[J].江苏农业科学,2017,45(20):279.
　Du Guojun,et al.Mutation breeding of high-yield pectinase-producing strain by microwave and DES[J].Jiangsu Agricultural Sciences,2017,45(7):279.
[8]高大响,黄小忠.1株黑曲霉固态发酵豆渣生产纤维素酶及淀粉酶工艺的优化[J].江苏农业科学,2017,45(22):218.
　Gao Daxiang,et al.Optimization of production process of cellulase and amylase by solid state fermentation of soybean residue using a Aspergillus niger strain[J].Jiangsu Agricultural Sciences,2017,45(7):218.
[9]聂金梅,李阳源,刘金山,等.黑曲霉葡萄糖氧化酶基因改造及其在毕赤酵母中的表达[J].江苏农业科学,2018,46(20):17.
　Nie Jinmei,et al.Expression of modified GOD gene from Aspergillus niger in Pichia pastoris[J].Jiangsu Agricultural Sciences,2018,46(7):17.
[10]王勇,张育铭,朱洪磊,等.高效纤维素降解菌的筛选及产酶活力测定[J].江苏农业科学,2020,48(23):255.
　Wang Yong,et al.Screening of high-efficiency cellulose-degrading bacteria and determination of enzyme-producing activity[J].Jiangsu Agricultural Sciences,2020,48(7):255.

备注/Memo

备注/Memo:
收稿日期：2025-04-10
基金项目：浙江省自然科学基金(编号：LGN22C200009)；嘉兴市科技计划(编号：2024AD10035)。
作者简介：陈欣怡(2001—)，女，浙江金华人，硕士研究生，主要从事天然产物活性开发研究。E-mail：m1184849036@163.com。
通信作者：李加友，博士，教授，主要从事中药功能因子挖掘及食药同源产品开发研究。E-mail：lijiayou@zjxu.edu.cn。
更新日期/Last Update: 2026-04-05