|本期目录/Table of Contents|

[1]曲斌,耿士伟,冯群科,等.超高效液相色谱测定鸡饲料中的5种类胡萝卜素添加剂[J].江苏农业科学,2014,42(09):273-275.
　Qu Bin,et al.Determination of five carotenoids additives in poultry feed by ultra performance liquid chromatography[J].Jiangsu Agricultural Sciences,2014,42(09):273-275.
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超高效液相色谱测定鸡饲料中的5种类胡萝卜素添加剂(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年09期
页码:
273-275
栏目:
质量安全与检测分析
出版日期:
2014-09-25

文章信息/Info

Title:
Determination of five carotenoids additives in poultry feed by ultra performance liquid chromatography
作者:
曲斌;  耿士伟;  冯群科;  吴玲;  贡玉清;  姜加华
江苏省畜产品质量检测测试中心，江苏南京 210000
Author(s):
Qu Bin; et al
关键词:
类胡萝卜素; 饲料; 超高效液相色谱
Keywords:
-
分类号:
O657.7+2
DOI:
-
文献标志码:
A
摘要:
研究并建立了鸡饲料中角黄素、虾青素等5种类胡萝卜素的超高效液相色谱分析法。采用乙腈提取饲料样品后，将提取液直接进行进样分析。色谱柱为Waters BEH C18 (1.7 m，2.1 mm×100 mm)；流动相为0.1%甲酸水溶液和0.1%甲酸乙腈溶液，采用梯度洗脱；检测波长450 nm；流速0.25 mL/min。结果表明，5种类胡萝卜素在 50~500 ng/mL范围内呈良好的线性关系，检测限为0.01~0.10 mg/kg，定量限为0.03~0.40 mg/kg，回收率80%~105%，精密度小于15%，说明超高效液相色谱分析法操作简便，适宜用于测定鸡饲料中的类胡萝卜素。
Abstract:
-

参考文献/References:

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［7］余孔捷，钱疆，杨方，等. 高效液相色谱法测定动物源性食品中角黄素、虾青素的研究［J］. 食品与发酵工业，2008，34(3)：145-148.
［8］Inbaraj B S，Chien J T，Chen B H. Improved high performance liquid chromatographic method for determination of carotenoids in the microalga Chlorella pyrenoidosa［J］. Journal of Chromatography A，2006，1102(1/2)：193-199.
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［10］Rivera S M，Canela-Garayoa R. Analytical tools for the analysis of carotenoids in diverse materials［J］. Journal of Chromatography A，2012，1224：1-10.

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备注/Memo

备注/Memo:
收稿日期：2013-12-04
基金项目：江苏省“六大人才高峰”高层次人才项目(编号：NY-062)。
作者简介：曲斌(1979—)，男，辽宁盘锦人，博士，兽医师，研究方向为兽药残留分析新技术与新方法。E-mail：qubin2000@hotmail.com。
更新日期/Last Update: 2014-09-25