|本期目录/Table of Contents|

[1]葛辰,凌去非,李彩娟,等.基于第二代测序的大鳞副泥鳅生长相关SNP标记的开发[J].江苏农业科学,2014,42(10):21-27.
 Ge Chen,et al.Development of growth-associated SNP markers of Paramisgurnus dabryanus based on next-generation sequencing[J].Jiangsu Agricultural Sciences,2014,42(10):21-27.
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基于第二代测序的大鳞副泥鳅生长相关SNP标记的开发(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年10期
页码:
21-27
栏目:
生物技术
出版日期:
2014-10-25

文章信息/Info

Title:
Development of growth-associated SNP markers of Paramisgurnus dabryanus based on next-generation sequencing
作者:
葛辰 凌去非 李彩娟 叶竹青 韩晓飞 王国成 朱鹏飞
苏州大学医学部基础医学与生物科学学院,江苏苏州 215123
Author(s):
Ge Chenet al
关键词:
大鳞副泥鳅高通量测序SNP多态性标记引物设计
Keywords:
-
分类号:
S917.4
DOI:
-
文献标志码:
A
摘要:
大鳞副泥鳅是鳅科重要经济鱼类,通过Illumina HiSeq 2000测序平台对2个大鳞副泥鳅样本的转录本进行了高通量测序,其中AB2011M-1是群体中生长较快的10尾鱼的混合转录本,AB2011M-2是生长较慢的10尾鱼的混合转录本。通过对以上2个混合转录本的测序,分别获得了65 536和80 786个单核苷酸多态性(single nucleotide polymorphisms,SNP)突变;对2个测序样本进行表达差异的比较,获得了39个表达差异基因。随机选取120个位点设计引物,进行验证分析,共获得16个有效的多态性标记。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期:2013-12-24
基金项目:江苏省农业科技自主创新资金[编号:CX(13)2042];江苏省科技支撑计划(编号:BE2012354);江苏省水产三项工程(编号:PJ2011-62)。
作者简介:葛辰(1989—),男,江苏苏州人,硕士研究生,主要从事水生动物种质资源保护与利用研究。E-mail:sishen0129@163.com。
通信作者:凌去非,博士,副教授,主要从事鱼类遗传育种与保护生物学研究。E-mail:lingqf@suda.edu.cn。
更新日期/Last Update: 2014-10-25