|本期目录/Table of Contents|

[1]马俊红,李军营,马二登,等.烤烟不同生育期蔗糖代谢关键酶基因的表达[J].江苏农业科学,2015,43(05):32-34.
 Ma Junhong,et al.Expression of key enzymes genes during sucrose metabolism in different growth period of tobacco[J].Jiangsu Agricultural Sciences,2015,43(05):32-34.
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烤烟不同生育期蔗糖代谢关键酶基因的表达(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第43卷
期数:
2015年05期
页码:
32-34
栏目:
生物技术
出版日期:
2015-05-25

文章信息/Info

Title:
Expression of key enzymes genes during sucrose metabolism in different growth period of tobacco
作者:
马俊红 李军营 马二登 卢秀萍
云南省烟草农业科学研究院,昆明云南 650031
Author(s):
Ma Junhonget al
关键词:
烤烟生育期蔗糖代谢转化酶蔗糖合成酶蔗糖磷酸合成酶基因表达
Keywords:
-
分类号:
S572.01
DOI:
-
文献标志码:
A
摘要:
为了研究烤烟不同生育期蔗糖代谢的分子特点,采用SYBR Green I 实时荧光定量RT-PCR法对云烟87在不同生育期的3种蔗糖代谢关键酶基因——转化酶(invertase,Inv)、蔗糖合成酶(sucrose synthase,SuSy)和蔗糖磷酸合成酶(sucrose phosphate synthase,SPS)基因的表达水平进行分析。结果表明:在烟叶发育前期(团棵期、旺长期)Inv酶基因的表达量较高;SuSy酶基因的在烤烟不同生育期的表达量变化呈单峰曲线,在打顶后表达量最高,之后又降低。SPS酶基因的表达量在烤烟生长发育后期较高,说明烤烟蔗糖的累积主要在发育后期进行。
Abstract:
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参考文献/References:

[1]张槐苓,葛翠英,穆怀静,等. 烟草分析与检验[M]. 郑州:河南科学技术出版社,1994:103-111.
[2]王瑞新. 烟草化学[M]. 北京:中国农业出版社,2003:42-43.
[3]云南省烟草科学研究所,中国烟草育种研究(南方)中心. 云南烟草栽培学[M]. 北京:科学出版社,2007:38-39.
[4]Farrar J,Pollock C,Gallagher J.Sucrose and the integration of metabolism in vascular plants[J]. Plant Sci,2000,154:1-11.
[5]Jang J C,Sheen J.Sugar sensing in higher plants[J]. Plant Cell,1994,6(1):1665-1679.
[6]Vauglm M W,Harrington G N,Bush D R.Sucrose-mediated transcriptional regulation of sucrose symporter activity in the phloem[J]. Proc Nad Acad Sd USA,2002,99:10876-10880.
[7]Zourelidou M,de Torres-Zabala M,Smith C,et al.Storekeeper defines a new clam of plant-specific DNA-binding proteins and is a putative regulator of patatin expression[J]. Plant J,2002,30:489-497.
[8]王宁宁,朱建新,王淑芳,等. 苦参碱对小麦旗叶中蔗糖磷酸合成酶活性的调节[J]. 南开大学学报:自然科学版,2000,33(1):19-22.
[9]张玉,陈昆松,张上隆,等. 猕猴桃果实采后成熟过程中糖代谢及其调节[J]. 植物生理与分子生物学学报,2004,30 (3):317-324.
[10]黄东亮,李双喜,廖青,等. 植物蔗糖磷酸合成酶研究进展[J]. 中国生物工程杂志,2012,32(6):109-119.
[11]McCollum T G,Huber D J,and Cantliffe D J.Soluble sugar accumulation and activity of related enzymes during musk melon fruit development[J]. Amer Soc Hort Sci,1988,113:399-403.
[12]张明方,李志凌. 高等植物中与蔗糖代谢相关的酶[J]. 植物生理学通讯,2002,38(3):289-295.
[13]Roitsch T,Tanner W. Cell wall invertase:bridging the gap[J]. Botanica Acta,1996,109(2):90-93.
[14]Bachelier C,Granam J,Manoir J du,et a1.Integration of an invertase gene to control sucrose metabolism in strawberry cultivars[J]. Acta Horticul,1997,439:161-163.
[15]李玉潜,谢九生,谭中文. 甘蔗叶片碳、氮代谢与产量、品质关系研究初探[J]. 中国农业科学,1995,28(4):46-53.
[16]魏庆华,杜铮,刘卫群,等. 河南不同地区烤烟蔗糖含量及其代谢关键酶之间的关系分析[J]. 江西农业学报,2011,23(1):6-8,13.
[17]Huber S C. Role of sucrose-phosphate synthase in partitioning of carbon in leaves[J]. Plant Physiol,1983,71:818-821
[18]Estruch J J,Beltran J P. Changes in invertase activities precede ovary growth induced by gibberellic acid in Pisum sativum[J]. Physid Plata,1991,81:319-326.
[19]Ricardo C P P,Aprees T. Invertase activity during the development of carrot roots[J]. Phytochemistry,1970,9:239-247.
[20]Schaffer A A. Invertase in young and mature leaves of Citrus sinensis[J]. Phytochemistry,1986,25:2275-2277.
[21]Xu D P,Sun g S J S,Black C C.Sucrose metabolism in lima bean seeds[J]. Plata Physio1,1989,89:1106-1116.

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备注/Memo

备注/Memo:
收稿日期:2014-05-27
基金项目:中国烟草总公司云南省公司资助项目“云南清香型特色优质烟叶开发项目”(编号:2011YN02);中国烟草总公司资助项目“清香型特色优质烟叶开发项目”[编号:110201101003(TS-03)]。
作者简介:马俊红(1976—),女,山西晋中人,博士,副教授,主要从事特色优质烟叶开发和烟草病害分子诊断等研究。Tel:(0871)65106539;E-mail:majunhong.china@gmail.com。
更新日期/Last Update: 2015-05-25