«上一篇/Previous Article|本期目录/Table of Contents|下一篇/Next Article»

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第44卷

期数:

2016年05期

页码:

186-189

栏目:

植物保护

出版日期:

2016-05-25

文章信息/Info

Title:

Identification and preliminary study on antifungal activity of lipopeptide produced by bacillus amyloliquefaciens B15

作者:

朱弘元¹;  康健¹;  范昕²;  郭聃洋²;  王德良²

1.新疆大学生命科学与技术学院，新疆乌鲁木齐 830000；2.中国食品发酵工业研究院，北京 100000

Author(s):

Zhu Hongyuan; et al

关键词:

脂肽; 解淀粉芽孢杆菌; 分离纯化; 抑菌机理; 鉴定

Keywords:

-

分类号:

TS201.3

DOI:

-

文献标志码:

A

摘要:

从葡萄表皮分离获得1株对霉菌具有明显抑制作用的解淀粉芽孢杆菌B15，对其发酵液经过酸沉、Sephadex LH-20层析柱，对抑菌脂肽的特性进行研究。结果发现，该菌株产脂肽对葡萄灰霉病菌有较好的抑制效果，60～100 ℃ 处理或pH 值为2～10的脂肽对灰霉病菌的抑菌活性几乎没有影响；脂肽有效最低抑菌浓度为0.017 mg/mL。经高效液相色谱法(HPLC)和质谱分析发现，解淀粉芽孢杆菌B15产脂肽主要活性成分为iturin A、fengycin。

Abstract:

-

参考文献/References:

［1］Maget-Dana R，Peypoux F I. A special class of pore-forming lipopeptides：biological and physicochemical properties［J］. Toxicology，1994，87(1)：151-174.
［2］Arima K，Kakinuma A，Surfactin T G. A crystalline peptidelipid surfactant produced by bacillus subtilis：isolation，characterization and its inhibition of fibrin clot formation［J］. Biochemical and Biophysical Research Communications，1968，31(3)：488-494.
［3］Hiradate S，Yoshida S，Sugie H，et al. Mulberry anthracnose antagonists (iturins) produced by Bacillus amyloliquefaciens RC-2［J］. Phytochemistry，2002，61(6)：693-698.
［4］Koumoutsi A，Chen X H，Henne A，et al. Structural and functional characterization of gene clusters directing nonribosomal synthesis of bioactive cyclic lipopeptides in Bacillus amyloliquefaciens strain FZB42［J］. Journal of Bacteriology，2004，186(4)：1084-1096.
［5］Caldeira A T，Santos A M，Coelho A V，et al. Combined use of LC-ESI-MS and antifungal tests for rapid identification of bioactive lipopeptides produced by Bacillus amyloliquefaciens CCMI 1051［J］. Process Biochemistry，2011，46(9)：1738-1746.
［6］Hathout Y，Ho Y P，Ryzhov V，et al. Kurstakins：a new class of lipopeptides isolated from Bacillus thuringiensis［J］. Journal of Natural Products，2000，63(11)：1492-1496.
［7］Perez C，Suarez C，Castro G R. Antimicrobial activity determined in strains of Bacillus circulans cluster［J］. Folia Microbiologica，1993，38(1)：25-28.
［8］Nishikiori T，Naganawa H，Muraoka Y，et al. Plipastatins：new inhibitors of phospholipase A2，produced by Bacillus cereus BMG302-fF67. Ⅲ. structural elucidation of plipastatins［J］. The Journal of Antibiotics，1986，39(6)：755-761.
［9］Morikawa M，Ito M，Imanaka T. Isolation of a new surfactin producer Bacillus-pumilus A-1，and cloning and nucleotide-sequence of the regulator gene，PSF-1［J］. Journal of Fermentation and Bioengineering，1992，74(5)：255-261.
［10］Sen R. Biotechnology in petroleum recovery：the microbial EOR［J］. Progress in Energy and Combustion Science，2008，34(6)：714-724.
［11］Das P，Mukherjee S，Sen R. Improved bioavailability and biodegradation of a model polyaromatic hydrocarbon by a biosurfactant producing bacterium of marine origin［J］. Chemosphere，2008，72(9)：1229-1234.
［12］Das P，Mukherjee S，Sen R. Biosurfactant of Marine origin exhibiting heavy metal remediation properties［J］. Bioresource Technology，2009，100(20)：4887-4890.
［13］Maity J P，Lin T J，Cheng H P H，et al. Synthesis of brushite particles in reverse microemulsions of the biosurfactant surfactin［J］. International Journal of Molecular Sciences，2011，12(6)：3821-3830.
［14］Singh B R，Dwivedi S，Al-Khedhairy A A，et al. Synthesis of stable cadmium sulfide nanoparticles using surfactin produced by Bacillus amyloliquifaciens strain KSU-109［J］. Colloids and Surfaces B-Biointerfaces，2011，85(2)：207-213.
［15］Mukherjee A K. Potential application of cyclic lipopeptide biosurfactants produced by Bacillus subtilis strains in laundry detergent formulations［J］. Letters in Applied Microbiology，2007，45(3)：330-335.
［16］Nitschke M，Costa S . Biosurfactants in food industry［J］. Trends in Food Science & Technology，2007，18(5)：252-259.
［17］Mukherjee S，Das P，Sivapathasekaran C，et al. Antimicrobial biosurfactants from marine Bacillus circulans：extracellular synthesis and purification［J］. Letters in Applied Microbiology，2009，48(3)：281-288.
［18］Rodrigues L，Banat I M，Teixeira J，et al. Biosurfactants：potential applications in medicine［J］. Journal of Antimicrobial Chemotherapy，2006，57(4)：609-618.
［19］Sivapathasekaran C，Das P，Mukherjee S A，et al. Marine bacterium derived lipopeptides：characterization and cytotoxic activity against cancer cell lines［J］. International Journal of Peptide Research and Therapeutics，2010，16(4)：215-222.
［20］Rangarajan V，Dhanarajan G，Sen R. Improved performance of cross-flow ultrafiltration for the recovery and purification of Ca²⁺ conditioned lipopeptides in diafiltration mode of operation［J］. Journal of Membrane Science，2014，454(15)：436-443.
［21］Hsieh F C，Lin T C，Meng M，et al. Comparing methods for identifying Bacillus strains capable of producing the antifungal lipopeptide iturin A［J］. Curr Microbiol，2008，56(1)：1-5.
［22］刘三军，蒯传化. 我国葡萄病害发生趋势与防治工作中应注意的若干问题［J］. 果农之友，2007(2)：4-5.

相似文献/References:

[1]张荣胜,戴秀华,陈志谊.解淀粉芽孢杆菌Lx-11对水稻细菌性条斑病的防治效果[J].江苏农业科学,2014,42(10):115.
　Zhang Rongsheng,et al.Control effect of Bacillus amyloliquefaciens Lx-11 on rice bacterial leaf streak[J].Jiangsu Agricultural Sciences,2014,42(05):115.
[2]屈俊廷,金海强,沈国娟,等.人参锈腐病生防用解淀粉芽孢杆菌Y-S-Y12菌株发酵条件的优化[J].江苏农业科学,2016,44(03):158.
　Qu Junting,et al.Optimization of fermentation conditions of Bacillus amyloliquefaciens srain Y-S-Y12 for biocontrol of ginseng rust rot[J].Jiangsu Agricultural Sciences,2016,44(05):158.
[3]王超,李宏伟,王翠,等.防治大白菜软腐病细菌菌株的筛选与鉴定[J].江苏农业科学,2013,41(12):114.
　Wang Chao,et al.Screening and identification of antagonistic bacteria controlling Chinese cabbage soft rot[J].Jiangsu Agricultural Sciences,2013,41(05):114.
[4]孙梅,张维娜,高亮,等.解淀粉芽孢杆菌JSSW-LA的分离鉴定及对病原菌拮抗特性[J].江苏农业科学,2016,44(04):275.
　Sun Mei,et al.Isolation， identification and pathogen antagonist properties of Bacillus amyloliquefaciens JSSW-LA[J].Jiangsu Agricultural Sciences,2016,44(05):275.
[5]裘纪莹,陈相艳,吴发萍,等.解淀粉芽孢杆菌NCPSJ7菌株高产抗菌物质的发酵条件优化[J].江苏农业科学,2016,44(01):311.
　Qiu Jiying,et al.Optimization of high production fermentation conditions of antimicrobial substance by Bacillus amyloliquefaciens strain NCPSJ7[J].Jiangsu Agricultural Sciences,2016,44(05):311.
[6]高同国,姜军坡,郭晓军,等.马铃薯疮痂病高效拮抗菌的筛选及鉴定[J].江苏农业科学,2016,44(12):157.
　Gao Tongguo,et al.Screening and identification of antagonistic bacteria against Streptomyces scabies on potato scab[J].Jiangsu Agricultural Sciences,2016,44(05):157.
[7]郭政宏,周彪,严亨秀.藏猪源解淀粉芽孢杆菌的益生性、安全性和应用性研究[J].江苏农业科学,2017,45(14):137.
　Guo Zhenghong,et al.Study on probiotic function，safety and application of Bacillus amyloliquefaciens from Tibetan pigs[J].Jiangsu Agricultural Sciences,2017,45(05):137.
[8]魏蜜,张伟,管维轩,等.1株油茶真菌病害拮抗菌株的筛选鉴定及其拮抗作用[J].江苏农业科学,2017,45(18):100.
　Wei Mi,et al.Screening and identification of an antagonistic strain of Camellia oleifera fungi disease and its antagonistic effect[J].Jiangsu Agricultural Sciences,2017,45(05):100.
[9]汪晶晶,曹雪梅,李欢,等.抗菌海洋解淀粉芽孢杆菌GM-1-2菌株摇瓶发酵培养基和发酵条件优化[J].江苏农业科学,2018,46(08):291.
　Wang Jingjing,et al.Optimization of medium components and cultural conditions of marine Bacillus amyloliquefaciens GM-1-2 strain with antifungal activity[J].Jiangsu Agricultural Sciences,2018,46(05):291.
[10]刘伟杰,尤琰婷,赵若菲,等.生物表面活性剂生产及应用的研究进展[J].江苏农业科学,2018,46(24):15.
　Liu Weijie,et al.Research progress on production and application of biosurfactant[J].Jiangsu Agricultural Sciences,2018,46(05):15.

备注/Memo

备注/Memo:

收稿日期：2015-03-31
基金项目：中国食品发酵工业研究院博士生专项基金。
作者简介：朱弘元(1986—)，男，江苏无锡人，硕士研究生，主要从事食品生物技术研究。E-mail：394872441@qq.com。
通信作者：康健，博士，副教授，主要从事食品加工技术研究，E-mail：kangjian505@sina.com；王德良，博士，教授级高工，主要从事食品发酵技术研究，E-mail：wdlpost@163.com。

常用功能

更新日期/Last Update: 2016-05-25