|本期目录/Table of Contents|

[1]乔洁,张春丹,毕利东,等.高效液相色谱法测定土壤中麦角固醇的含量[J].江苏农业科学,2017,45(04):153-155.
　Qiao Jie,et al.Determination of ergosterol contents in soil sample by high performance liquid chromatography[J].Jiangsu Agricultural Sciences,2017,45(04):153-155.
点击复制

高效液相色谱法测定土壤中麦角固醇的含量(PDF)
分享到：

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年04期
页码:
153-155
栏目:
贮藏加工与检测分析
出版日期:
2017-02-20

文章信息/Info

Title:
Determination of ergosterol contents in soil sample by high performance liquid chromatography
作者:
乔洁1; 张春丹1; 毕利东2; 尧水红3; 赵英4
1.廊坊师范学院生命科学学院，河北廊坊 065000； 2.河海大学农业工程学院，江苏南京 210098；
3.中国农业科学院农业资源与农业区划研究所，北京 100081； 4.西北农林科技大学资源环境学院，陕西杨凌 712100
Author(s):
Qiao Jie; et al
关键词:
土壤; 真菌; 麦角固醇; 高效液相色谱; 测定方法; KOH-醇溶液提取法; 正己烷-异丙醇提取法
Keywords:
-
分类号:
S154.3
DOI:
-
文献标志码:
A
摘要:
麦角固醇是真菌和一些藻类细胞膜中的主要固醇类物质，真菌死亡后迅速分解，因此它的含量能够用来表征土壤真菌的活性。由于麦角固醇受土壤真菌种类及其生长环境的影响显著且较易被分解，因此建立一种简单、快速、准确的测定麦角固醇含量的方法尤为重要。比较KOH-醇溶液提取法和正己烷-异丙醇提取法2种不同测定方法发现，不通过皂化处理不但不会影响测定的准确率，而且还会大大缩短提取时间，减小提取过程中麦角固醇的损失，从而显著提高回收率。同时，麦角固醇测定方法的回收率被证实与土壤样品水土比之间存在显著的负相关关系(r=-0943，P<0.05)。
Abstract:
-

参考文献/References:

［1］Mccaig A，Grayston S，Prosser J，et al. Impact of cultivation on characterisation of species composition of soil bacterial communities［J］. FEMS Microbiology Ecology，2001，35(1)：37-48.
［2］Borneman J，Skroch P，Osullivan K，et al. Molecular microbial diversity of an agricultural soil in Wisconsin［J］. Applied and Environmental Microbiology，1996，62(6)：1935-1943.
［3］王曙光，侯彦林. 磷脂脂肪酸方法在土壤微生物分析中的应用［J］. 微生物学通报，2004，31(1)：114-117.
［4］van Elsas J D，Duarte G F，Rosado A S，et al. Microbiological and molecular biological methods for monitoring microbial inoculants and their effects in the soil environment［J］. Mcrobiol Meth，1998，32：133-154.
［5］张汉波，段昌群，屈良鹄. 非培养方法在土壤微生物生态学研究中的应用［J］. 生态学杂志，2003，22(5)：131-136.
［6］Ruzicka S，Edgerton D，Norman M，et al. The utility of ergosterol as a bioindicator of fungi in temperate soils［J］. Soil Biology & Biochemistry，2000，32(7)：989-1005.
［7］West A W，Grant W D，Sparling G P. Use of ergosterol，diaminopimelic acid and glucosamine contents of soils to monitor changes in microbial populations［J］. Soil Biol Biochem，1987，19：607-612.
［8］Davis M W，Lamar R T. Evaluation of methods to extract ergosterol for quantification of soil fungus biomass［J］. Soil Biol Biochem，1992，24：189-198.
［9］Seitz L M，Mohr H E，Burroughs R，et al. Ergosterol as an indicator of fungal invasion in grains［J］. Cereal Chemistry，1997，54：1207-1217.
［10］Stahl P D，Parkin T B. Relationship of soil ergosterol concentration and fungal biomass［J］. Soil Biol Biochem，1996，28：847-855.
［11］Barajas-Aceves M，Hassan M，Tinoco R，et al. Effect of pollutants on the ergosterol content as indicator of fungal biomass［J］. Journal of Microbiological Methods，2002，50(3)：227-236.
［12］Grant W D，West A W. Measurement of ergosterol，diaminopimelic acid and glucosamine in soil： evaluation as indicators of microbial biomass［J］. Journal of Microbiological Methods，1986，6：47-53.
［13］Zhao X R，Lin Q，Brookes P C. Does soil ergosterol concentration provide a reliable estimate of soil fungal biomass?［J］. Soil Biology & Biochemistry，2005，37(2)：311-317.
［14］Ruzicka S，Norman M D P，Harris J A. Short communication rapid ultrasonication method to determine ergosterol concentration in soil［J］. Soil Biol Biochem，1995，27：1215-1517.
［15］Ruzicka S，Norman M D，Harris J A. Rapid ultrasonication method to determine ergosterol concentration in soil［J］. Soil Biology & Biochemistry，1995，27(9)：1215-1217.
［16］Young I M，Illian J，Harris J A，et al. Comment on“Does ergosterol concentration provide a reliable estimate of soil fungal biomass?”［J］. Soil Biology & Biochemistry，2006，38(6)：1500-1501.

相似文献/References:

[1]张美景,杨硕,陈艳萍,等.玉米黑粉菌单倍体菌株的分离及交配型a位点的鉴别[J].江苏农业科学,2013,41(04):122.
[2]蒋宝南,刘腾飞,单建明,等.QuEChERS-GC/μECD法测定土壤中的毒死蜱残留量[J].江苏农业科学,2014,42(12):332.
　Jiang Baonan,et al.Determination of chlorpyrifos residues in soil by QuEChERS-GC/μECD[J].Jiangsu Agricultural Sciences,2014,42(04):332.
[3]李国锋,魏瑞成,王冉.高效液相色谱法测定土壤中联苯与间羟基苯甲酸残留[J].江苏农业科学,2014,42(12):316.
　Li Guofeng,et al.Determination of biphenyl and M-hydroxy benzoic acid residues in soil by high performance liquid chromatography[J].Jiangsu Agricultural Sciences,2014,42(04):316.
[4]史景允,于伟红,梁秋生.蓖麻对镉污染土壤的修复潜力[J].江苏农业科学,2014,42(11):386.
　Shi Jingyun,et al(8).Potential repairing of cadmium contaminated soil by castor oil plant[J].Jiangsu Agricultural Sciences,2014,42(04):386.
[5]何继山,梁杏,李静.土样浸提液电导率与盐分关系的逐步回归分析[J].江苏农业科学,2014,42(10):314.
　He Jishan,et al.Regression analysis of relationship between soil samples leaching solution conductivity and solinity[J].Jiangsu Agricultural Sciences,2014,42(04):314.
[6]徐洪文,卢妍.土壤碳矿化及活性有机碳影响因子研究进展[J].江苏农业科学,2014,42(10):4.
　Xu Hongwen,et al.Research progress on soil carbon mineralization and factors affecting active organic carbon[J].Jiangsu Agricultural Sciences,2014,42(04):4.
[7]李范,李娜,陈建中,等.基于磷脂脂肪酸提取方法的微生物群落结构研究[J].江苏农业科学,2014,42(09):323.
　Li Fan,et al.Study on microbial community structure based on phospholipid fatty acid extraction method[J].Jiangsu Agricultural Sciences,2014,42(04):323.
[8]张乐森,刘悦上,马金芝,等.山东省滨州市设施蔬菜土壤退化防治与修复对策[J].江苏农业科学,2013,41(07):141.
　Zhang Lesen,et al.Control and restoration strategies of facility vegetable soil degradation in Binzhou of Shandong Province[J].Jiangsu Agricultural Sciences,2013,41(04):141.
[9]尹辉,李晖,蒋忠诚,等.典型岩溶区土壤水分的空间异质性研究[J].江苏农业科学,2013,41(07):332.
　Yin Hui,et al.Study on spatial variability of soil water content in typical karst area[J].Jiangsu Agricultural Sciences,2013,41(04):332.
[10]覃怀德,吴炳孙,吴敏,等.橡胶园土壤钾素空间变异与分区管理技术——以海南省琼中县为例[J].江苏农业科学,2013,41(08):326.
　Qin Huaide,et al.Spatial variability and regionalized management of soil potassium nutrient in rubber plantation—Taking Qiongzhong County of Hainan Province as an example[J].Jiangsu Agricultural Sciences,2013,41(04):326.
[11]赵帆,赵密珍,王钰,等.草莓不同连作年限土壤养分及微生物区系分析[J].江苏农业科学,2017,45(16):110.
　Zhao Fan,et al.Analysis of nutrients and microbial flora of strawberry soil with different continuous cropping years[J].Jiangsu Agricultural Sciences,2017,45(04):110.
[12]漆艳香,张欣,彭军,等.不同抗、感枯萎病香蕉种质根际土壤的微生物数量[J].江苏农业科学,2019,47(13):110.
　Qi Yanxiang,et al.Microbial population in rhizosphere soil of different banana germplasms with Fusarium oxysporum f. sp. cubense resistance[J].Jiangsu Agricultural Sciences,2019,47(04):110.
[13]谭骏,黄河,汤薇,等.蚯蚓粪有机肥对土壤微生物群落的影响[J].江苏农业科学,2021,49(20):228.
　Tan Jun,et al.Effect of earthworm manure organic fertilizer on soil microbial community[J].Jiangsu Agricultural Sciences,2021,49(04):228.

备注/Memo

备注/Memo:
收稿日期：2015-12-28
基金项目：河北省高等学校科学技术研究青年基金(编号：QN2015169)；河北省廊坊市科学技术项目(编号：2013012004)；廊坊师范学院青年基金(编号：LSLQ201406)；河北省高校食药用菌应用技术研发中心项目(编号：YF201411)；廊坊师范学院微生物学重点学科项目(编号：2015001)。
作者简介：乔洁(1980—)，女，吉林白山人，硕士，讲师，主要从事微生物生态学研究。E-mail：jieqiao1980@126.com。
通信作者：赵英，博士，副教授，硕士生导师，主要从事环境土壤学研究。E-mail：yzhao@gmail.com。
更新日期/Last Update: 2017-02-20