基于转录组的大头金蝇密码子的偏好性分析-《江苏农业科学》

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Title:: Analysis of codon usage preference of Chrysomya megacephala based on transcriptome

作者:: 张玉波¹; 2; 周正湘¹; 2; 吴小玉¹; 张斌³; 1.安顺学院农学院，贵州安顺 561000； 2.贵州省昆虫信息系统与资源开发利用重点实验，贵州安顺 561000；
3.内蒙古师范大学生命科学与技术学院，内蒙古呼和浩特 010022

Author(s):: Zhang Yubo; et al

关键词:: 大头金蝇; 转录组; 密码子偏好性; 同义密码子

Keywords:: -

分类号:: Q969.451.9；S186

DOI:: -

文献标志码:: A

摘要:: 使用Codon W软件分析大头金蝇［Chrysomya megacephala(Fabricius，1794)］转录组10 923条全长转录序列的密码子偏好性。结果表明，大头金蝇转录组中的AT含量(6181%)远大于GC含量(38.19%)；PR2(parity rule 2，即密码子偏好性)绘图结果显示，密码子第3位碱基A的使用频率大于碱基T，碱基G的使用频率大于碱基C；中性绘图结果显示，该序列密码子的使用更多地受到选择压力的影响；有效密码子数(effective number of codons，简称ENC)在25.17~61.00个之间，均值为43.16个；密码子适应指数(codon adaptation index，简称CAI)在0.099~0.554之间，均值为0.215 8。结果共筛选出29个同义密码子相对使用度(relative synonymous codon usage，简称RSCU)>1的密码子和28个最优密码子。

Abstract:: -

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