|本期目录/Table of Contents|

[1]席继锋,袁立岗,杨楠,等.奶牛性别控制试验的效果验证[J].江苏农业科学,2019,47(23):195-197.
　Xi Jifeng,et al.Verification on effect of sex control experiment for dairy cattle[J].Jiangsu Agricultural Sciences,2019,47(23):195-197.
点击复制

奶牛性别控制试验的效果验证(PDF)
分享到：

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第47卷
期数:
2019年第23期
页码:
195-197
栏目:
畜牧兽医与水产蚕桑
出版日期:
2019-12-30

文章信息/Info

Title:
Verification on effect of sex control experiment for dairy cattle
作者:
席继锋1;  袁立岗2;  杨楠4;  邓双义1;  贾斌3;  张永生3;  李超程3;  夏欢3;  王香祖1
1.新疆农业职业技术学院，新疆昌吉 831100； 2.新疆生产建设兵团第十二师兽医站，新疆乌鲁木齐 830000；
3.石河子大学动物科技学院，新疆石河子 832003； 4.新疆天山畜牧生物工程股份有限公司，新疆昌吉 831100
Author(s):
Xi Jifeng; et al
关键词:
奶牛; 性别控制; 试验; 效果验证
Keywords:
-
分类号:
S823.3
DOI:
-
文献标志码:
A
摘要:
为满足畜牧生产需求，更有效地控制奶牛的后代性别，采用RNA干扰技术分别对2头成年健康繁殖性能正常的荷斯坦公牛Zfy基因mRNA进行干扰，应用SPSS 19.0统计软件进行卡方检验分析方法。结果显示，与对照组相比，干扰组公牛的后代性别比例发生明显的偏移，母犊比例达75%以上，而子代的体尺、生长发育及繁殖性能等均无变化。得出结论，干扰Zfy基因生产奶牛性控精液的方法简单、方便、易操作，在生产中具有一定的推广价值和应用前景。
Abstract:
-

参考文献/References:

［1］杨增明，孙青原，夏国良. 生殖生物学［M］. 北京：科学出版社，2005：53-54.
［2］Reynolds R，Varlaro J. Gender determination of forensic samples using PCR amplification of ZFY/ZFX gene sequence［J］. Journal of Forensic Sciences，41(2)：276-286.
［3］Vergnaud G，Page D C，Simmler M C，et al. A deletion map of the human Y chromosome based on DNA hybridization［J］. Am J Human Genet，1986，38(2)：109-109.
［4］Ao A，Frickson R P，Winston R，et al. Transcription of paternal Y linked genes in the human zygote as early as the prenudeate stage［J］. Zygote，1994，2(4)：281.
［5］Nagamine C M，Chan K M，Kozak C A，et al. Chromosome mapping and expression of a putative testis-determining gene in mouse［J］. Science，1989，2434(4887)：80-83.
［6］Mardon G，Page D C. The sex-determining region of the mouse Y chromosome encodes a protein with a highly acidic domain and 13 zinc fingers［J］. Cell，1989，s6(5)：765-770.
［7］彭强，班谦，贾斌，等. siRNA介导小鼠Zfy基因干扰的定量分析［J］. 石河子大学学报(自然科学版)，2011，29(4)：448-451.
［8］Page D C，Mosher R，Simpson E M，et al.The sex-determining region of the human Y chromosome encodes a finger protein［J］. Cell，1987. 51(6)：1091-1104.
［9］Vernet N，Mahadevaiah S K，Decarpentrie F A，et al. Mouse Y-encoded transcription factor Zfy2 is essential for sperm head remodelling and sperm tail development［J］. PLoS One，2016，11(1)：1-15.
［10］Vernet N，Mahadevaiah S K，Ojarikre O A，et al. The Y-Encoded gene Zfy2 zcts to remove cells with unpaired chromosomes at the first meiotic metaphase in male mice［J］. Current Biology，2011，21(9)：787-793.
［11］Vernet N，Mahadevaiah S K，Yamauchi Y A，et al. Mouse Y-Linked Zfy1 and Zfy2 are expressed during the Male-Specific interphase between meiosis Ⅰ and meiosis Ⅱ and promote the 2nd meiotic division［J］. PLoS Genetics，2014，10(6)：1-15.
［12］Vernet N，Szot M，Mahadevaiah S K，et al. The expression of Y-linked Zfy2 in XY mouse oocytes leads to frequent meiosis 2 defects，a high incidence of subsequent early cleavage stage arrest and infertility［J］. Development，2014，141(4)：855-866.
［13］Vernet N，Mahadevaiah S K，de Rooij D G，Et al. Zfy genes are required for efficient meiotic sex chromosome inactivation (MSCI) in spermatocytes［J］. Human Molecular Genetics，2016，25(24)：5300-5310.
［14］申立山，贺业春，杨春林，等. 小白鼠生精上皮周期的形成［J］. 哈尔滨医科大学学报，1991，25(4)：241-243.

相似文献/References:

[1]黎江,蒙翠萍,周悦,等.2种奶牛隐性乳房炎检测方法的对比[J].江苏农业科学,2013,41(05):193.
　Li Jiang,et al.Comparative study on two detection methods of recessive mastitis of dairy cow[J].Jiangsu Agricultural Sciences,2013,41(23):193.
[2]刘俊栋,刘海霞,赵佳,等.挥发性脂肪酸对奶牛离体真胃平滑肌收缩的影响[J].江苏农业科学,2013,41(06):166.
　Liu Jundong,et al.Effect of volatile fatty acids on smooth muscle contraction of cow abomasal in vitro[J].Jiangsu Agricultural Sciences,2013,41(23):166.
[3]孙宝丽,陈汝能,吴银宝,等.不同季节、不同生理阶段奶牛粪便的特性[J].江苏农业科学,2014,42(08):187.
　Sun Baoli,et al.Characteristics of dairy cow dung during different seasons and physiological stages[J].Jiangsu Agricultural Sciences,2014,42(23):187.
[4]耿丽微,李泉辉.奶牛数字化虚拟仪器测控网络USB的通信设计[J].江苏农业科学,2016,44(03):431.
　Geng Liwei,et al.USB communication design and research of milk cow digitization virtual instrument measurement and control network[J].Jiangsu Agricultural Sciences,2016,44(23):431.
[5]徐俊,侯玉洁,丁健,等.不同牧草来源及纤维水平对奶牛的影响研究进展[J].江苏农业科学,2013,41(10):153.
　Xu Jun,et al.Research progress for effect of forage sources and fiber levels on dairy cow[J].Jiangsu Agricultural Sciences,2013,41(23):153.
[6]周帮会,翟向和,陈斌,等.奶牛发生胎衣不下的胎盘免疫学及中药防治机理[J].江苏农业科学,2013,41(10):181.
　Zhou Banghui,et al.Placental immunology and traditional Chinese herbal medicine prevention mechanism for retention of dairy cows’ afterbirth[J].Jiangsu Agricultural Sciences,2013,41(23):181.
[7]胡路锋,刘贤侠,王少华,等.应用B超辅助诊断奶牛临床子宫疾病[J].江苏农业科学,2013,41(12):229.
　Hu Lufeng,et al.Application of B-mode ultrasonography in diagnosis of clinical uterus diseases of dairy cows[J].Jiangsu Agricultural Sciences,2013,41(23):229.
[8]邓敏,梁光华,徐雷,等.促孕散治疗持久黄体不孕奶牛的血清微量元素与激素相关性分析[J].江苏农业科学,2014,42(02):173.
　Deng Min,et al.Correlation analysis of trace elements and hormones in serum of persistent corpus luteum dairy cattle treated by fertility-promoting powder[J].Jiangsu Agricultural Sciences,2014,42(23):173.
[9]尹柏双,王秋竹,付连军,等.隐性乳房炎奶牛乳清中部分酶活性变化[J].江苏农业科学,2016,44(07):276.
　Yin Baishuang,et al.Activity change of some enzyme in whey of dairy cows with recessive mastitis[J].Jiangsu Agricultural Sciences,2016,44(23):276.
[10]王军,彭永帅,霍军,等.郑州市奶牛隐性乳腺炎病原菌的分离与鉴定[J].江苏农业科学,2016,44(09):254.
　Wang Jun,et al.Isolation and identification of subclinical mastitis of dairy cattle in Zhengzhou City[J].Jiangsu Agricultural Sciences,2016,44(23):254.

备注/Memo

备注/Memo:
收稿日期：2018-09-06
基金项目：2017年度新疆农业职业技术学院资助课题(编号：XJNZYKJ201704)；新疆生产建设兵团第十二师科技专项(编号：NYJH2013010)。
作者简介：席继锋(1978—)，男，甘肃人，博士，副教授，主要从事动物遗传育种与繁殖研究。E-mail：fftt2011@163.com。
通信作者：王香祖，博士，副教授，主要从事动物遗传育种与繁殖研究。E-mail：173897302@qq.com。
更新日期/Last Update: 2019-12-05