|本期目录/Table of Contents|

[1]李美茹,杨烁烁,张洪琛,等.红线虫抗菌肽提取条件的工艺优化[J].江苏农业科学,2020,48(17):191-194.
 Li Meiru,et al.Optimization of extraction conditions of antibacterial peptides from red nematodes[J].Jiangsu Agricultural Sciences,2020,48(17):191-194.
点击复制

红线虫抗菌肽提取条件的工艺优化(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第48卷
期数:
2020年第17期
页码:
191-194
栏目:
贮藏加工与检测分析
出版日期:
2020-09-05

文章信息/Info

Title:
Optimization of extraction conditions of antibacterial peptides from red nematodes
作者:
李美茹 杨烁烁 张洪琛 张天悦 吴兴华 张会会
廊坊师范学院生命科学学院/河北省动物多样性重点实验室,河北廊坊 065000
Author(s):
Li Meiruet al
关键词:
红线虫抗菌肽抑菌效果大肠杆菌金黄色葡萄球菌
Keywords:
-
分类号:
Q516;S188
DOI:
-
文献标志码:
A
摘要:
以红线虫(Limnodrilus hoffmeisteri)为材料,采用0.01 mol/L EDTA-2Na的0.9% NaCl溶液为浸提液提取有抑菌活性的多肽,以料液比、时间、pH值对红线虫抗菌肽提取条件进行3因素3水平正交试验优化提取工艺,并对处理结果进行多重比较。试验结果表明,红线虫抗菌肽对大肠杆菌没有抑菌效果,对金黄色葡萄球菌有一定抑制效果;影响浸提效果的主要因素为料液比,次要因素为浸提时间和pH值,提取的最优处理组合:料液比1 g ∶15 mL,浸提时间8 h,浸提液pH值为7.2。
Abstract:
-

参考文献/References:

[1]马文山,廖富苹,范雪云,等. 细菌抗菌肽的研究进展及其应用[J]. 生物技术通报,2008(3):39-43,49.
[2]Boman H B. Inducible antibacterial defense system in Drasophial[J]. Natural,1972,237(5352):232-235.
[3]李文楚. 昆虫抗菌肽研究进展[J]. 广东蚕业,2000,34(2):51-59.
[4]黄自然,王少颐. 注射大肠杆菌诱导柞蚕蛹血淋巴产生抗菌物质(简报)[J]. 华南农学院学报,1981,2(1):65-68.
[5]Huang Z R,Zheng T H,Ling Y Z,et al. Inhibition effects of antibacterial peptided from the Chinese oak silkworm(Antheraea pernyi)pupae[J]. Science Bulletin,1987,32(9):629-632.
[6]Dourado F S,Leite J R,Silva L P,et al. Antimicrobial peptide from the skin secretion of the frog Leptodactylus syphax[J]. Toxicom,2007,50(4):572-580.
[7]马清泉,董娜,曹艳萍,等. 利用精氨酸和缬氨酸设计新型 α-螺旋抗菌肽[J]. 微生物学报,2011,51(3):346-351.
[8]王宏亮,李昂,那杰. 抗菌肽在果蔬保鲜中的应用[J]. 江苏农业科学,2014,42(3):238-240.
[9]林碧敏,曹庸. 抗菌肽在农业和食品工业的应用[J]. 广东蚕业,2013(3):47-51.
[10]Lu J,Chen Z W. Isolation,characterization and anti-cancer activity of SK84,a novel glycine-rich antimicrobial peptide from Drosophila virilis[J]. Peptides,2010,31(1):44-50.
[11]Shaykhiev R,Beisswenger C K,et al. Human endogenous antibiotic LL-37 stimulates airway epithelial cell proliferation and wound closure[J]. American Journal of Physiology(Lung Cellular and Molecular and Molecular Physiology),2005,33(5):L842-L848.
[12]Choi J,Sabikhi L,Hassan A,et al. Bioactive peptedes in dairy productes[J]. Interantional Journary of Dairy Technology,2012,65(1):1-12.

相似文献/References:

[1]王宏亮,李昂,那杰.抗菌肽在果蔬保鲜中的应用[J].江苏农业科学,2014,42(03):238.
 Wang Hongliang,et al.Application of antimicrobial peptides in preservation of fruits and vegetables[J].Jiangsu Agricultural Sciences,2014,42(17):238.
[2]武慧,宋明霞,宁思淇,等.紫花苜蓿的抗菌肽基因Rev4遗传转化[J].江苏农业科学,2017,45(07):44.
 Wu Hui,et al.Genetic transformation of antimicrobial peptide gene Rev4] in Medicago sativa[J].Jiangsu Agricultural Sciences,2017,45(17):44.
[3]谢昆,王丽仙,王靖,等.家蚕抗菌肽基因BmMoricin的克隆及重组蛋白的自诱导表达[J].江苏农业科学,2017,45(21):52.
 Xie Kun,et al.Cloning of BmMoricin antibacterial peptide gene and auto-induction expression of recombinant proteins from Bombyx mori[J].Jiangsu Agricultural Sciences,2017,45(17):52.
[4]卢艳敏.猪感染PRRSV后Cathelicidins抗菌肽基因的表达情况[J].江苏农业科学,2018,46(02):24.
 Lu Yanmin.Expression of Cathelicidins antibacterial peptide gene after porcine infected with PRRSV[J].Jiangsu Agricultural Sciences,2018,46(17):24.
[5]杭柏林,徐军,胡建和.鸡内源性抗菌肽LEAP-2的生物信息学分析[J].江苏农业科学,2018,46(06):46.
 Hang Bolin,et al.Bioinformatics analysis of endogenous antimicrobial peptide LEAP-2 of chicken[J].Jiangsu Agricultural Sciences,2018,46(17):46.
[6]吴三桥,赵冠杰,万健,等.抗菌肽SMAP-29在大肠杆菌中表达条件的优化及纯化研究[J].江苏农业科学,2018,46(09):44.
 Wu Sanqiao,et al.Optimization of expression conditions and purification of antibacterial peptide SMAP-29 in Escherichia coli[J].Jiangsu Agricultural Sciences,2018,46(17):44.
[7]张丽君,王英,张燕,等.小分子海洋抗菌肽鲎素Ⅰ里氏木霉表达系统构建[J].江苏农业科学,2018,46(14):41.
 Zhang Lijun,et al.Construction of Trichoderma reesei expression system for a small molecular marine antibacterial peptide tachyplesin Ⅰ[J].Jiangsu Agricultural Sciences,2018,46(17):41.

备注/Memo

备注/Memo:
收稿日期:2019-10-10
基金项目:廊坊师范学院校级项目(编号:LSLY201404)。
作者简介:李美茹(1974—),女,河北秦皇岛人,硕士,副教授,从事多肽分离纯化研究。E-mali:lmrdyx@163.com。
更新日期/Last Update: 2020-09-05