|本期目录/Table of Contents|

[1]唐文文,陈垣.党参地上茎叶总黄酮提取工艺及其抗氧化活性[J].江苏农业科学,2021,49(17):171-177.
 Tang Wenwen,et al.Optimization of extraction process of total flavonoids from stems and leaves of Codonopsis pilosula and the evaluation of their antioxidation activities[J].Jiangsu Agricultural Sciences,2021,49(17):171-177.
点击复制

党参地上茎叶总黄酮提取工艺及其抗氧化活性(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第49卷
期数:
2021年第17期
页码:
171-177
栏目:
贮藏加工与检测分析
出版日期:
2021-09-05

文章信息/Info

Title:
Optimization of extraction process of total flavonoids from stems and leaves of Codonopsis pilosula and the evaluation of their antioxidation activities
作者:
唐文文12陈垣2
1.铜仁职业技术学院,贵州铜仁 554300; 2.甘肃农业大学农学院,甘肃兰州 730070
Author(s):
Tang Wenwenet al
关键词:
党参茎叶总黄酮提取工艺双酶协同超声法响应面抗氧化
Keywords:
-
分类号:
R284
DOI:
-
文献标志码:
A
摘要:
以党参废弃物茎叶为试验材料,在单因素试验基础上,设计Box-Behnken响应面试验,优化双酶协同超声波提取党参茎叶总黄酮的最佳提取工艺,采用清除DPPH自由基、ABTS自由基和 FRAP法评价党参茎叶总黄酮的抗氧化活性。结果表明,利用双酶协同超声法提取党参茎叶总黄酮的最佳条件为:液料比40 mL ∶1 g,乙醇体积分数70%,双酶(纤维素酶 ∶果胶酶=1 ∶1)用量0.45 mg/mL,酶解时间60 min,超声时间42 min,此条件下,总黄酮提取率为6.511%。DPPH自由基、ABTS自由基和FRAP总还原力的分析结果表明,党参茎叶总黄酮具有较高的抗氧化活性,在浓度为100 μg/mL时,对DPPH自由基清除率达到91.74%;在浓度达到500 μg/mL时,对ABTS自由基的清除率为94.82%,非常接近维生素C;党参茎叶总黄酮的总还原力为28.22 mg/g。本研究确定出党参茎叶总黄酮具有抗氧化活性,可为党参资源的充分利用提供数据支撑。
Abstract:
-

参考文献/References:

[1]于小红,赵嵘,代云桃,等. 党参标准汤剂质量评价的建立[J]. 中国实验方剂学杂志,2017,23(7):24-29.
[2]吴仪洛. 本草从新:十八卷[M]. 新1版.上海:上海卫生出版社,1957:5.
[3] 国家药典委员会. 中国药典:一部[M]. 北京:中国医药科技出版社,2020:293.
[4]Aga E B,Li H J,Chen J,et al.Chemical constituents from the aerial parts of Codonopsis nervosa[J]. Chinese Journal of Natural Medicines,2012,10(5):366-369.
[5]成铭,皮文霞,陆兔林,等. 党参地上部分的党参炔苷、多糖含量测定及影响因素分析[J]. 中药材,2020,43(5):1092-1098
[6]蔡兴航,孙晓春,孙安敏,等. 党参茎叶总皂苷提取工艺及其抗氧化活性研究[J]. 中国农学通报,2018,34(26):146-151.
[7]Lin L Z,Zhuang M Z,Lei F F,et al.GC/MS analysis of volatiles obtained by headspace solid-phase microextraction and simultaneous-distillation extraction from Rabdosia serra (MAXIM.) HARA leaf and stem[J]. Food Chemistry,2013,136(2):555-562.
[8]陈蓬凤,梅新,黄师荣,等. 不同品种薯尖的总酚、总黄酮含量及抗氧化活性比较[J]. 现代食品科技,2021,37(3):132-138,15.
[9]Dong X Z,Huang Y Y,Wang Y H,et al. Anti-inflammatory and antioxidant jasmonates and flavonoids from lychee seeds[J]. Journal of Functional Foods,2019,54:74-80.
[10]de Souza D R,Willems J L,Low N H. Phenolic composition and antioxidant activities of saskatoon berry fruit and pomace[J]. Food Chemistry,2019,290:168-177.
[11]Jin L,Li X B,Tian D Q,et al. Antioxidant properties and color parameters of herbal teas in China[J]. Industrial Crops and Products,2016,87:198-209.
[12]杨硕,徐元庆,邢媛媛,等. 植物源黄酮类化合物对动物免疫和抗氧化功能影响的研究进展[J]. 动物营养学报,2019,31(7):2958-2964.
[13]王英,张玉刚,戴洪义. 苹果果皮中类黄酮的超声波辅助提取及稳定性研究[J]. 食品科学,2011,32(16):178-181.
[14]李侠,邹基豪,王大为. 响应面试验优化超声波-酶法提取绿豆皮黄酮类化合物工艺[J]. 食品科学,2017,38(8):206-212.
[15]何国耀,王建国,郭福存,等. 党参茎叶的急、慢性毒性试验[J]. 中国兽医科技,1988,18(1):53 -54.

相似文献/References:

[1]何可群,李相兴.民族药金丝梅总黄酮含量的测定[J].江苏农业科学,2013,41(05):307.
 He Kequn,et al.Determination of total flavonoids content in ethnic drug Hypericum patulum Thunb.[J].Jiangsu Agricultural Sciences,2013,41(17):307.
[2]石雪萍,李小华,杨爱萍.南京雨花茶中总黄酮提取以及DPPH自由基清除活性研究[J].江苏农业科学,2013,41(06):238.
 Shi Xueping,et al.Extraction and DPPH free radicals scavenging activity of total flavonoids from Yuhua tea of Nanjing[J].Jiangsu Agricultural Sciences,2013,41(17):238.
[3]周银丽,王晓云,白建波,等.芭蕉花中总黄酮的最佳提取工艺[J].江苏农业科学,2013,41(06):257.
 Zhou Yinli,et al.Optimum extraction technology for total flavonoids from flowers of Japanese bananaes[J].Jiangsu Agricultural Sciences,2013,41(17):257.
[4]梁梓,杜兵,汪淑芳,等.九节龙总黄酮的抑菌作用[J].江苏农业科学,2013,41(06):294.
 Liang Zi,et al.Antibacterial effect of total flavonoids in Ardisia pusilla[J].Jiangsu Agricultural Sciences,2013,41(17):294.
[5]冯海燕,任 蕾,贾鹏飞,等.超声波辅助提取柿蒂总黄酮的工艺[J].江苏农业科学,2013,41(07):275.
 Feng Haiyan,et al.Technology of total flavonoids extraction from Kaki calyx by ultrasonic assisted extraction method[J].Jiangsu Agricultural Sciences,2013,41(17):275.
[6]张虹,白红丽,张江梅,等.青叶胆不同部位总黄酮提取及其抑菌作用[J].江苏农业科学,2013,41(09):224.
 Zhang Hong,et al.Extraction of total flavonoids from different spots of Swertia mileensis and its antibacterial effect[J].Jiangsu Agricultural Sciences,2013,41(17):224.
[7]王亚红,祝波.微波法提取狼把草总黄酮工艺研究[J].江苏农业科学,2014,42(04):235.
 Wang Yahong,et al.Study on microwave-assisted extraction process of total flavonoid from Bidens frondosa[J].Jiangsu Agricultural Sciences,2014,42(17):235.
[8]尤静,任晓芳,刘春叶,等.菊花中总黄酮成分不同提取工艺的比较[J].江苏农业科学,2014,42(05):218.
 You Jing,et al.Comparison of different extraction process of general flavonoids from chrysanthemum[J].Jiangsu Agricultural Sciences,2014,42(17):218.
[9]任冰如,陈剑,吕寒,等.红凤菜总黄酮的富集方法[J].江苏农业科学,2014,42(06):260.
 Ren Bingru,et al.Enrichment method of total flavonids from Gynura bicolor[J].Jiangsu Agricultural Sciences,2014,42(17):260.
[10]房海灵,卢艳花,聂[XCZ.tif],等.响应面法优化广东紫珠总黄酮的超声波提取工艺[J].江苏农业科学,2014,42(07):265.
 Fang Hailing,et al.Optimization of ultrasonic extraction process of total flavonoid from Callicarpa kwangtungensis Chun. by response surface methodology[J].Jiangsu Agricultural Sciences,2014,42(17):265.

备注/Memo

备注/Memo:
收稿日期:2021-06-30
基金项目:国家重点研发专项(编号:2018YFC1706301);甘肃省现代农业中药材产业体系首席专家项目(编号:GARS-ZYC-1)。
作者简介:唐文文(1986—),女,河南许昌人,硕士,副教授,主要从事药用植物资源开发与利用研究。E-mail:412251544@qq.com。
通信作者:陈垣,博士,教授,主要从事药用植物资源开发与利用研究。E-mail:chenyuan@gsau.edu.cn。
更新日期/Last Update: 2021-09-05