|本期目录/Table of Contents|

[1]葛金涛,王江英,汤雪燕,等.百合叶片磷脂酶基因家族转录组学分析[J].江苏农业科学,2022,50(3):36-42.
　Ge Jintao,et al.Transcriptome analysis of phospholipase gene family in lily leaves[J].Jiangsu Agricultural Sciences,2022,50(3):36-42.
点击复制

百合叶片磷脂酶基因家族转录组学分析(PDF)
分享到：

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第3期
页码:
36-42
栏目:
“转录组”专题
出版日期:
2022-02-05

文章信息/Info

Title:
Transcriptome analysis of phospholipase gene family in lily leaves
作者:
葛金涛1;  王江英1;  汤雪燕1;  腾年军2;  朱朋波1;  孙明伟1;  赵统利1;  邵小斌1
1.连云港市农业科学院，江苏连云港 222000； 2.南京农业大学园艺学院，江苏南京 210095
Author(s):
Ge Jintao; et al
关键词:
百合; 叶烧病; 磷脂酶; 注释基因; 转录组测序; 蛋白质数据库
Keywords:
-
分类号:
S682.2+65.01
DOI:
-
文献标志码:
A
摘要:
为探究磷脂酶基因家族在百合叶片中的表达情况，利用Illumina测序对百合正常叶片和叶烧病叶片进行转录组测序分析。测序共获得磷脂酶注释基因274个，分为PLA1、PLA2、PLB、PLC和PLD共5类。在KEGG、NR、COG和Swiss-prot等4个蛋白质数据库中都注释为磷脂酶基因的Unigene序列有34条，其中PLA2、PLB、PLC和PLD类磷脂酶基因参与醚脂类代谢、甘油磷脂代谢调控，在百合叶烧病发生过程中，PLA2基因与PLC基因表达量上调，PLB与PLD类既有上调表达基因也有下调表达基因。
Abstract:
-

参考文献/References:

［1］Berghoef J. Effect of calcium on tipburn of Lilium ‘Pirate’［J］. Acta Hort，1986，177(61)：433-438.
［2］Chang Y C，Miller W B.The relationship between leaf enclosure，transpiration，and upper leaf necrosis on Lilium ‘Star Gazer’［J］. Journal of the American Society for Horticultural Science，2004，129(1)：128-133.
［3］杨阳，王晶懋. 百合响应非生物胁迫的分子机制研究［J］. 分子植物育种，2018，16(15)：5046-5054.
［4］Campos M L.A novel role for a phospholipase D in plant immunity［J］. Plant Physiology，2020，183(1)：33-34.
［5］李晓薇，戢舒涵，赵旭，等. 亚麻芥未成熟胚中磷脂酶基因家族的转录组学分析［J］. 中国油料作物学报，2018，40(6)：793-800.
［6］Wang G L，Ryu S，Wang X M.Plant phospholipases：an overview［J］. Methods in Molecular Biology，2012，861：123-137.
［7］Hong Y Y，Pan X Q，Welti R，et al. The effect of phospholipase Dalpha3 on Arabidopsis response to hyperosmotic stress and glucose［J］. Plant Signaling & Behavior，2008，3(12)：1099-1100.
［8］Jang J H，Lee O R.Overexpression of ginseng patatin-related phospholipase pPLAIIIβ alters the polarity of cell growth and decreases lignin content in Arabidopsis［J］. Journal of Ginseng Research，2020，44(2)：321-331.
［9］Hong Y Y，Zhao J A，Guo L A，et al. Plant phospholipases D and C and their diverse functions in stress responses［J］. Progress in Lipid Research，2016，62：55-74.
［10］Gonorazky G，Guzzo M C，Abd-El-haliem A M，et al. Silencing of the tomato phosphatidylinositol-phospholipase C2 (SlPLC2) reduces plant susceptibility to Botrytis cinerea［J］. Molecular Plant Pathology，2016，17(9)：1354-1363.
［11］Shi X，Chye M L . An Arabidopsis family of six acyl-CoA-binding proteins has three cytosolic members［J］. Plant Physiology & Biochemistry，2009，47(6)：479-484.
［12］Manevich Y，Fisher A B. Peroxiredoxin 6，a 1-Cys peroxiredoxin，functions in antioxidant defense and lung phospholipid metabolism［J］. Free Radical Biology & Medicine，2005，38(11)：1422-1432.
［13］Scherer G F E，Ryu S B，Wang X M，et al. Patatin-related phospholipase A：nomenclature，subfamilies and functions in plants［J］. Trends in Plant Science，2010，15(12)：693-700.
［14］Chen G Q，Greer M S，Lager I，et al. Identification and characterization of an LCAT-like Arabidopsis thaliana gene encoding a novel phospholipase A［J］. FEBS Letters，2012，586(4)：373-377.
［15］Ryu S B. Phospholipid-derived signaling mediated by phospholipase A in plants［J］. Trends in Plant Science，2004，9(5)：229-235.
［16］Holk A，Rietz S，Zahn M，et al. Molecular identification of cytosolic，patatin-related phospholipases A from Arabidopsis with potential functions in plant signal transduction［J］. Plant Physiology，2002，130(1)：90-101.
［17］Gao W，Li H Y，Xiao S，et al. Protein interactors of acyl-CoA-binding protein ACBP2 mediate cadmium tolerance in Arabidopsis［J］. Plant Signaling & Behavior，2010，5(8)：1025-1027.
［18］Wang A J，Johnson C A，Jones Y，et al. Subcellular localization and PKC-dependent regulation of the human lysophospholipase A/acyl-protein thioesterase in WISH cells［J］. Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids， 2000，1484(2/3)：207-214.
［19］Hirano T，Kishi M，Sugimoto H，et al. Thioesterase activity and subcellular localization of acylprotein thioesterase 1/lysophospholipase 1［J］. Biochimica et Biophysica Acta-Molecular and Cell Biology of Lipids，2009，1791(8)：797-805.
［20］Meldrum E，Parker P J，Carozzi A.The PtdIns-PLC superfamily and signal transduction［J］. Biochimica et Biophysica Acta (-Molecular Cell Research，1991，1092(1)：49-71.
［21］Kocourková D，Krcˇková Z，Pejchar P，et al. The phosphatidylcholine-hydrolysing phospholipase C NPC4 plays a role in response of Arabidopsis roots to salt stress［J］. Journal of Experimental Botany，2011，62(11)：3753-3763.
［22］Pokotylo I，Kolesnikov Y，Kravets V，et al. Plant phosphoinositide-dependent phospholipases C：Variations around a canonical theme［J］. Biochimie，2014，96：144-157.
［23］Salinas-Mondragon R E，Kajla J D，Perera I Y，et al. Role of inositol 1，4，5-triphosphate signalling in gravitropic and phototropic gene expression［J］. Plant，Cell & Environment，2010，33(12)：2041-2055.
［24］Wang X M，Devaiah S P，Zhang W H，et al. Signaling functions of phosphatidic acid［J］. Progress in Lipid Research，2006，45(3)：250-278.
［25］杨学利，马沁沁，袁向华，等. 磷脂酶D及其催化机制的研究进展［J］. 中国油脂，2016，41(3)：80-84.

相似文献/References:

[1]吴祝华,詹德智,施季森,等.百合尖孢镰刀菌鳞茎腐烂病研究进展[J].江苏农业科学,2013,41(06):1.
　Wu Zhuhua,et al.Research progress of basal rotting disease of lily caused by Fusarium oxysporum[J].Jiangsu Agricultural Sciences,2013,41(3):1.
[2]关惠忆,雷家军,吴杉.多安娜×大花卷丹种间杂种花粉母细胞减数分裂行为观察[J].江苏农业科学,2013,41(09):164.
　Guan Huiyi,et al.Pollen mother cells meiotic behavior observation of interspecific hybrid of Duoanna×Lilium leichtlinii Hook. f. var. maximowiczii (Regel) Baker[J].Jiangsu Agricultural Sciences,2013,41(3):164.
[3]张静,左丹,唐道城.百合花粉的形态特征观察及温度对其萌发率的影响[J].江苏农业科学,2014,42(01):148.
　Zhang Jing,et al.Observation of morphological characteristics of lily pollens and effect of temperature on its germination[J].Jiangsu Agricultural Sciences,2014,42(3):148.
[4]胡新颖,杨迎东,颜津宁,等.百合鳞片全基质包埋试验[J].江苏农业科学,2014,42(02):147.
　Hu Xinying,et al.A full matrix embedding test of lily bud scale[J].Jiangsu Agricultural Sciences,2014,42(3):147.
[5]赵兴华,裴新辉,杨佳明,等.LA 和O型百合正反交试验及胚培养的杂交效果[J].江苏农业科学,2016,44(04):273.
　Zhao Xinghua,et al.LA and O type lily reciprocal cross experiment and hybrid effect of early embryo culture[J].Jiangsu Agricultural Sciences,2016,44(3):273.
[6]胡新颖,杨迎东,颜津宁,等.百合种球脱毒技术研究进展[J].江苏农业科学,2014,42(04):6.
　Hu Xinying,et al.Research progress of eradication techniques of viruses in lily[J].Jiangsu Agricultural Sciences,2014,42(3):6.
[7]童巧珍,高昱,刘平安,等.基于SRAP标记的湘产百合核心种质库构建[J].江苏农业科学,2016,44(01):56.
　Tong Qiaozhen,et al.Construction of Hunan lily core germplasm repository based on SRAP marker[J].Jiangsu Agricultural Sciences,2016,44(3):56.
[8]郭思佳,王倩,梁隐泉,等.几种百合花粉生活力测定及贮藏条件研究[J].江苏农业科学,2015,43(09):205.
　Guo Sijia,et al.Study on pollen viability measurement and storage conditions of several lily cultivars[J].Jiangsu Agricultural Sciences,2015,43(3):205.
[9]杨佳明,赵兴华,裴新辉.百合品种间杂交亲和性研究[J].江苏农业科学,2015,43(05):175.
　Yang Jiaming,et al.Study on cross-compatibility of Lilium cultivars[J].Jiangsu Agricultural Sciences,2015,43(3):175.
[10]方中明,于恒,白根祥,等.香水百合组织培养和快速繁殖条件的优化[J].江苏农业科学,2016,44(08):81.
　Fang Zhongming,et al.Optimization of tissue culture and rapid propagation conditions of perfume lily[J].Jiangsu Agricultural Sciences,2016,44(3):81.

备注/Memo

备注/Memo:
收稿日期：2021-05-28
基金项目：江苏省农业科技自主创新资金［编号：CX(20)3025］；连云港市农业科学院青年基金(编号：QNJJ2004)；江苏省政策引导类计划项目(苏北科技专项)(编号：SZ-LYG202039)。
作者简介：葛金涛(1987—)，男，山东安丘人，硕士，助理研究员，主要从事园艺作物育种研究。E-mail：672643419@qq.com。
通信作者：邵小斌，研究员，主要从事园艺作物育种与栽培技术研究。E-mail：382748523@qq.com。
更新日期/Last Update: 2022-02-05