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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第51卷

期数:

2023年第5期

页码:

11-18

栏目:

“转录组学” 专栏

出版日期:

2023-03-05

文章信息/Info

Title:

Transcriptome-wide identification and bioinformatics analysis of bHLH transcription factor family in onion (Allium cepa L.)

作者:

陈微¹; 潘美红¹; 惠林冲¹; 李威亚¹; 郇国磊²; 何林玉¹; 缪美华¹; 陈振泰¹; 杨海峰¹

1.江苏省连云港市农业科学院，江苏连云港 222000； 2.江苏省灌南县农业农村局，江苏灌南 222000

Author(s):

Chen Wei; et al

关键词:

洋葱; bHLH; 转录因子; 生物信息学分析

Keywords:

-

分类号:

S633.201

DOI:

-

文献标志码:

A

摘要:

bHLH转录因子家族是真核生物中重要的转录因子家族，在植物生长发育、次生代谢和逆境应答中发挥重要作用。本研究利用转录组数据对洋葱基因家族成员进行筛选和鉴定，并对家族成员进行理化性质、保守基序、亚细胞定位、系统发育和蛋白互作生物信息学分析，同时对bHLH基因家族在洋葱幼苗中的表达情况进行分析。结果表明，从洋葱转录组共鉴定到36个bHLH基因，预测编码蛋白质含有的氨基酸数量为127～610个，且均为亲水性蛋白，系统发育分析表明，洋葱bHLH基因家族分为12个亚族，同一亚族的大多数基因具有相似的保守基序，大多数bHLH 基因在洋葱幼苗中表达，不同基因的表达量水平差异较大。本研究结果为进一步研究洋葱bHLH 转录因子家族的生物学功能奠定了基础。

Abstract:

-

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备注/Memo

备注/Memo:

收稿日期：2022-04-21
基金项目：江苏省农业科技自主创新资金［编号：CX(20)3164］；连云港市财政专项(编号：QNJJ2005)。
作者简介：陈微(1990—)，女，山东菏泽人，硕士，助理研究员，主要从事蔬菜育种和栽培研究。E-mail：1135050599@qq.com。
通信作者：杨海峰，副研究员，主要从事蔬菜育种和栽培研究。E-mail：13775492959@163.com。

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更新日期/Last Update: 2023-03-05