|本期目录/Table of Contents|

[1]许芳芳,刘文祥,郑伟,等.茶树NF-Y基因家族鉴定及非生物胁迫下的表达分析[J].江苏农业科学,2023,51(5):81-93.
　Xu Fangfang,et al.Genome identification of Camellia sinensis NF-Y gene family and its expression analysis of abiotic stress[J].Jiangsu Agricultural Sciences,2023,51(5):81-93.
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第51卷
期数:
2023年第5期
页码:
81-93
栏目:
生物技术
出版日期:
2023-03-05

文章信息/Info

Title:
Genome identification of Camellia sinensis NF-Y gene family and its expression analysis of abiotic stress
作者:
许芳芳; 刘文祥; 郑伟; 孙耀清; 王辉
信阳农林学院林学院，河南信阳 464000
Author(s):
Xu Fangfang; et al
关键词:
茶树; NF-Y家族; 干旱胁迫; 全基因组分析; 基因表达
Keywords:
-
分类号:
S571.101
DOI:
-
文献标志码:
A
摘要:
NF-Y(nuclear factor-Y，NF-Y)通常是以NF-YA (CBF-B/HAP2)、NF-YB (CBF-A/HAP3)和NF-YC (CBF-C/HAP5) 3种亚基构成异源三聚体的形式调控下游基因的表达，并在生物与非生物胁迫耐受性等方面起着重要作用。本研究在茶树基因组中鉴定分类了茶树NF-Y基因家族成员，为后续的功能分析研究做准备。在研究中鉴定出45个NF-Y转录因子亚基(10个NF-YA，20个NF-YB，15个NF-YC)。染色体定位和同义分析表明，茶树NF-Y基因分布在14条染色体上，9对基因具有大片段复制，4对基因串联复制，片段复制是家族成员扩张的主要方式。通过序列分析，所有的NF-Y基因具有高度的结构保守性，大多数NF-Y基因有2个以上的内含子。通过启动子顺式元件分析发现，大部分基因含有激素和应激反应元件。转录组分析显示，一部分基因在干旱情况下出现不同程度的转录组丰度增加，而在盐处理后基本没有变化或出现不同程度的丰度下降。随后的qRT-PCR结果显示，在选定的4个基因中，CsNF-YA6、CsNF-YB4在20%PEG处理下出现不同幅度的上调，200 mmol/L 盐处理下，CsNF-YA6、CsNF-YB4、CsNF-YC5在盐处理后2 d出现轻微上调。结合qRT-PCR结果分析表明，在候选基因中存在介导茶树响应干旱胁迫与盐胁迫途径的基因。综上所述，茶树NF-Y基因在茶树非生物胁迫防御等多方面响应并发挥作用，本研究也为揭示相关NF-Y基因在茶树干旱胁迫与盐胁迫下的确切作用奠定了基础。
Abstract:
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备注/Memo

备注/Memo:
收稿日期：2022-08-04
基金项目：河南省林草局科技兴林项目(编号：YLK202138)；河南省科技攻关项目(编号：212102110186)；信阳农林学院青年基金(编号：QN2021013、QN2021016)。
作者简介：许芳芳(1993—)，女，河南驻马店人，硕士，助教，主要从事植物抗逆胁迫及生态保护研究。E-mail：906792515@qq.com。
通信作者：王辉，硕士，副教授，从事植物造景与养护研究与教学。E-mail：13733159426@163.com。
更新日期/Last Update: 2023-03-05