«上一篇/Previous Article|本期目录/Table of Contents|下一篇/Next Article»

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第52卷

期数:

2024年第1期

页码:

41-48

栏目:

生物技术

出版日期:

2024-01-05

文章信息/Info

Title:

Bioinformatics and expression analysis of TCP gene family in Punica granatum L.

作者:

李晓静¹; 李圣龙¹; 岳亮亮²

1.红河学院生物科学与农学学院/云南省高校滇南特色生物资源研究与利用重点实验室，云南蒙自 661100；2.西南林业大学高原湿地中心，云南昆明 650224

Author(s):

Li Xiaojing; et al

关键词:

石榴; TCP转录因子; 基因家族分析; 基因表达; 生物信息学

Keywords:

-

分类号:

S665.401

DOI:

-

文献标志码:

A

摘要:

TCP是植物特有转录因子家族之一，参与植物的叶发育、花发育、分枝发育、芽的休眠、果实的成熟及花色苷的生物合成等生长发育过程。本研究以石榴基因组数据和转录组数据为材料，分析了石榴TCP基因家族成员的理化性质、系统进化关系、保守结构域、基因结构和表达情况。结果表明，石榴基因组有22个TCP基因，TCP蛋白氨基酸长度在221~644 aa之间，蛋白分子量为23.23~70.76 ku。石榴TCP基因可分为ClassⅠ(PCF)和ClassⅡ(CYC/TB1和CIN)两大类。大多数PgTCP基因无内含子。CIN亚族成员PgTCP1、PgTCP3、PgTCP4、PgTCP5含有miR319调控位点。PgTCP1和PgTCP3在叶片中表达量高，PgTCP1和PgTCP14在花中表达量高。PgTCP22在果皮发育中表达量降低，PgTCP2和PgTCP5在外种皮发育中表达量降低。该研究结果可为石榴TCP家族基因功能研究，探索TCP调控石榴果实发育及品质形成提供参考，为石榴的分子育种提供科学依据。

Abstract:

-

参考文献/References:

［1］Wang Q J，Xu G X，Zhao X H，et al. Transcription factor TCP20 regulates peach bud endodormancy by inhibiting DAM5/DAM6 and interacting with ABF2［J］. Journal of Experimental Botany，2020，71(4)：1585-1597.
［2］Kieffer M，Master V，Waites R，et al. TCP14 and TCP15 affect internode length and leaf shape in Arabidopsis［J］. The Plant Journal，2011，68(1)：147-158.
［3］Hur Y S，Kim J，Kim S，et al. Identification of TCP13 as an upstream regulator of ATHB12 during leaf development［J］. Genes，2019，10(9)：644.
［4］Wang Y，Yu Y H，Wang J D，et al. Heterologous overexpression of the GbTCP5 gene increased root hair length，root hair and stem trichome density，and lignin content in transgenic Arabidopsis［J］. Gene，2020，758：144954.
［5］Davière J M，Wild M，Regnault T，et al. Class I TCP-DELLA interactions in inflorescence shoot apex determine plant height［J］. Current Biology，2014，24(16)：1923-1928.
［6］An J P，Liu Y J，Zhang X W，et al. Dynamic regulation of anthocyanin biosynthesis at different light intensities by the BT2-TCP46-MYB1 module in apple［J］. Journal of Experimental Botany，2020，71(10)：3094-3109.
［7］Xie Y G，Ma Y Y，Bi P P，et al. Transcription factor FvTCP9 promotes strawberry fruit ripening by regulating the biosynthesis of abscisic acid and anthocyanins［J］. Plant Physiology and Biochemistry，2020，146：374-383.
［8］Wei W，Hu Y，Cui M Y，et al. Identification and transcript analysis of the TCP transcription factors in the diploid woodland strawberry Fragaria vesca［J］. Frontiers in Plant Science，2016，7：1937.
［9］Jiu S T，Xu Y，Wang J Y，et al. Genome-wide identification，characterization，and transcript analysis of the TCP transcription factors in Vitis vinifera［J］. Frontiers in Genetics，2019，10：1276.
［10］Leng X，Wei H，Xu X，et al. Genome-wide identification and transcript analysis of TCP transcription factors in grapevine［J］. BMC Genomics，2019，20：786.
［11］Zheng A Q，Sun F L，Cheng T T，et al. Genome-wide identification of members of the TCP gene family in switchgrass (Panicum virgatum L.) and analysis of their expression［J］. Gene，2019，702：89-98.
［12］李圣龙，王传铭，李晓静.石榴NAC转录因子家族的生物信息学分析［J］. 分子植物育种，2021，19(1)：88-99.
［13］Chen C J，Chen H，Zhang Y，et al. TBtools：an integrative toolkit developed for interactive analyses of big biological data［J］. Molecular Plant，2020，13(8)：1194-1202.
［14］Qin G H，Xu C Y，Ming R，et al. The pomegranate (Punica granatum L.) genome and the genomics of punicalagin biosynthesis［J］. Plant Journal，2017，91(6)：1108-1128.
［15］Danisman S，van Dijk A D J，Bimbo A，et al. Analysis of functional redundancies within the Arabidopsis TCP transcription factor family［J］. Journal of Experimental Botany，2013，64(18)：5673-5685.
［16］Wang S T，Sun X L，Hoshino Y，et al. MicroRNA319 positively regulates cold tolerance by targeting OsPCF6 and OsTCP21 in rice (Oryza sativa L.)［J］. PLoS One，2014，9(3)：e91357.
［17］Shi P B，Guy K M，Wu W F，et al. Genome-wide identification and expression analysis of the ClTCP transcription factors in Citrullus lanatus［J］. BMC Plant Biology，2016，16：85.
［18］Violeta P，Marco B，Jacqueline B L，et al. Identification，cloning and characterization of the tomato TCP transcription factor family［J］. BMC Plant Biology，2014，14(1)：157.
［19］Yin Z，Li Y，Zhu W，et al. Identification，characterization，and expression patterns of TCP genes and microRNA319 in cotton［J］. International Journal of Molecular Sciences，2018，19(11)：3655.
［20］Wang H F，Wang H W，Liu R，et al. Genome-wide identification of TCP family transcription factors in Medicago truncatula reveals significant roles of miR319-targeted TCPs in nodule development［J］. Frontiers in Plant Science，2018，9：774.
［21］Zheng X Y，Yang J J，Lou T X，et al. Transcriptome profile analysis reveals that CsTCP14 induces susceptibility to foliage diseases in cucumber［J］. International Journal of Molecular Sciences，2019，20(10)：2582.
［22］Velasco R，Zharkikh A，Affourtit J，et al. The genome of the domesticated apple (Malus×domestica Borkh.)［J］. Nature Genetics，2010，42(10)：833-839.
［23］Consortium T G.The tomato genome sequence provides insights into fleshy fruit evolution［J］. Nature，2012，485(7400)：635-641.
［24］Xu R R，Sun P，Jia F J，et al. Genomewide analysis of TCP transcription factor gene family in Malus domestica［J］. Journal of Genetics，2014，93(3)：733-746.
［25］Tang C，Xie Y M，Guo M，et al. AASRA：an anchor alignment-based small RNA annotation pipeline［J］. Biology of Reproduction，2021，105(1)：267-277.
［26］Liu Y，Guan X Y，Liu S N，et al. Genome-wide identification and analysis of TCP transcription factors involved in the formation of leafy head in Chinese cabbage［J］. International Journal of Molecular Sciences，2018，19(3)：847.
［27］Rubio-Somoza I，Zhou C M，Confraria A，et al. Temporal control of leaf complexity by miRNA-regulated licensing of protein complexes［J］. Current Biology，2014，24(22)：2714-2719.
［28］Xu Y M，Xiao X M，Zeng Z X，et al. BrTCP7 transcription factor is associated with MeJA-promoted leaf senescence by activating the expression of BrOPR3 and BrRCCR［J］. International Journal of Molecular Sciences，2019，20(16)：3963.
［29］Koyama T，Nii H，Mitsuda N，et al. A regulatory cascade involving class ii ethylene response factor transcriptional repressors operates in the progression of leaf senescence［J］. Plant Physiology，2013，162(2)：991-1005.
［30］Koyama T，Sato F，Ohme-Takagi M.Roles of miR319 and TCP transcription factors in leaf development［J］. Plant Physiology，2017，175(2)：874-885.
［31］Schommer C，Palatnik J F，Aggarwal P，et al. Control of jasmonate biosynthesis and senescence by miR319 targets［J］. PLoS Biology，2008，6(9)：e230.
［32］Palatnik J F，Allen E，Wu X L，et al. Control of leaf morphogenesis by microRNAs［J］. Nature，2003，425(6995)：257-263.
［33］Guo Z H，Shu W S，Cheng H Y，et al. Expression analysis of TCP genes in peach reveals an involvement of PpTCP.A2 in ethylene biosynthesis during fruit ripening［J］. Plant Molecular Biology Reporter，2018，36(4)：588-595.

相似文献/References:

[1]陶吉寒,招雪晴,苑兆和,等.石榴DFR基因的同源克隆及分析[J].江苏农业科学,2013,41(04):22.
[2]郭文琦,张培通,李春宏,等.沿海滩涂绿化树种选择和耐盐性评价[J].江苏农业科学,2014,42(10):175.
　Guo Wenqi,et al.Selection and evaluation on salt tolerance of gardening tree species in coastal beach areas[J].Jiangsu Agricultural Sciences,2014,42(1):175.
[3]陈成,汪洪涛.山楂石榴复合果酒发酵工艺的研究[J].江苏农业科学,2013,41(07):248.
　Chen Cheng,et al.Study on fermentation technology of hawthorn and pomegranate compound fruit wine[J].Jiangsu Agricultural Sciences,2013,41(1):248.
[4]邓志勇,吴桂容,杨程显.脐橙-石榴复合果酒酿造工艺的研究[J].江苏农业科学,2015,43(02):266.
　Deng Zhiyong,et al.Study on brewing technology of compound wine of navel orange and pomegranate[J].Jiangsu Agricultural Sciences,2015,43(1):266.
[5]范春丽,罗青.干旱胁迫下外源甜菜碱对石榴光合作用、渗透调节及保护酶活性的影响[J].江苏农业科学,2016,44(11):229.
　Fan Chunli,et al.Effects of exogenous glycinebetaine on photosynthesis，osmotic adjustment ability and protective enzyme activity of Punica granatum under drought stress[J].Jiangsu Agricultural Sciences,2016,44(1):229.
[6]冯立娟,焦其庆,尹燕雷,等.石榴果皮DHQ/SDH基因的克隆及序列分析[J].江苏农业科学,2017,45(01):26.
　Feng Lijuan,et al.Cloning and sequence analysis of DHQ/SDH gene in pomegranate peel[J].Jiangsu Agricultural Sciences,2017,45(1):26.
[7]鲁海菊,李河,史淑義,等.云南省石榴干腐病病菌生物学特性及其防治药剂筛选[J].江苏农业科学,2017,45(01):99.
　Lu Haiju,et al.Study on biological characteristics of pathogen of pomegranate dry rot from Yunnan Province and its control fungicides[J].Jiangsu Agricultural Sciences,2017,45(1):99.
[8]曹尚银,牛娟,张杰,等.2种蛋白质组学方法在石榴中的应用比较[J].江苏农业科学,2017,45(20):68.
　Cao Shangyin,et al.A comparative study on application of two proteomics methods in pomegranate[J].Jiangsu Agricultural Sciences,2017,45(1):68.
[9]周银丽,郭建伟,杨伟,等.间作桃树对石榴园枯萎病土壤碳代谢多样性的影响[J].江苏农业科学,2018,46(14):106.
　Zhou Yinli,et al.Influences of intercropping peach trees on fusarium wilt soil carbon metabolism diversity in pomegranate garden[J].Jiangsu Agricultural Sciences,2018,46(1):106.
[10]王硕,李德生,朱秀锦,等.石榴对镉、铅、锌复合污染土壤的修复效果[J].江苏农业科学,2019,47(02):250.
　Wang Shuo,et al.Remediation effect of pomegranate on Cd，Pb and Zn combined pollution soil[J].Jiangsu Agricultural Sciences,2019,47(1):250.

备注/Memo

备注/Memo:

收稿日期：2023-02-20
基金项目：国家自然科学地区基金(编号：42161015)；云南省教育厅科学研究基金(编号：2023J1115)。
作者简介：李晓静(1986—)，女，河南洛阳人，博士，讲师，主要从事果树栽培学方面的教学与研究。E-mail：lanmeixj@163.com。
通信作者：岳亮亮，博士，助理研究员，主要从事植物学、生物地理学、湿地学、分子生态学方面的教学与研究。E-mail：yueliangliang@swfu.edu.cn。

常用功能

更新日期/Last Update: 2024-01-05