|本期目录/Table of Contents|

[1]冯立娟,焦其庆,尹燕雷,等.石榴果皮DHQ/SDH基因的克隆及序列分析[J].江苏农业科学,2017,45(01):26-29.
 Feng Lijuan,et al.Cloning and sequence analysis of DHQ/SDH gene in pomegranate peel[J].Jiangsu Agricultural Sciences,2017,45(01):26-29.
点击复制

石榴果皮DHQ/SDH基因的克隆及序列分析(PDF)
分享到:

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第45卷
期数:
2017年01期
页码:
26-29
栏目:
生物技术
出版日期:
2017-01-05

文章信息/Info

Title:
Cloning and sequence analysis of DHQ/SDH gene in pomegranate peel
作者:
冯立娟1 焦其庆1 尹燕雷1 李甲梁2
1.山东省果树研究所,山东泰安 271000;2.枣庄市农业科学研究院,山东枣庄 277300
Author(s):
Feng Lijuanet al
关键词:
石榴DHQ/SDH基因克隆序列分析
Keywords:
-
分类号:
S665.401;Q785
DOI:
-
文献标志码:
A
摘要:
以泰山红石榴果实为试验材料,利用逆转录PCR(RT-PCR)技术扩增出980 bp大小的中间片段,用 3′cDNA 末端快速扩增PCR(RACE PCR)获得DHQ/SDH基因的3′端,拼接后获得1 585 bp的cDNA片段。结果表明,该基因含有1 265 bp编码序列(CDS),编码421个氨基酸,其氨基酸序列与苹果(注册号:XP_008370537.1)、葡萄(注册号:XP_002270232.1)、白梨(注册号:XP_009335660.1)的同源性分别为82%、84%、82%;蛋白质理论分子质量为 133 682.2 u,等电点为5.01,含量最丰富的氨基酸是丙氨酸(Ala)、半胱氨酸(Cys)、甘氨酸(Gly)、苏氨酸(Thr),具有DHQase I、SDH(AroE)2个保守结构域,为亲水性蛋白;二级结构主要由无规则卷曲(31.12%)、α-螺旋(30.17%)、伸展链(24.94%)和β-转角(13.78%)组成;GenBank登录号为KU133479。
Abstract:
-

参考文献/References:

[1]Zhao X E,Yuan Z H,Feng L J,et al. Cloning and expression of anthocyanin biosynthetic genes in red and white pomegranate[J]. Journal of Plant Research,2015,128(4):687-696.
[2]Schuck A G,Weisburg J H,Esan H,et al. Cytotoxic and proapoptotic activities of gallic acid to human oral cancer HSC-2 cells[J]. Oxidants and Antioxidants in Medical Science,2013,2(4):265-274.
[3]Qiu X B,Takemura G,Koshiji M,et al. Gallic acid induces vascular smooth muscle cell death via hydroxyl radical production[J]. Heart and Vessels,2000,15(2):90-99.
[4]Zaidi-Yahiaoui R,Zaidi F,Bessai A A. Influence of gallic and tannic acids on enzymatic activity and growth of Pectobacterium chrysanthemi(Dickeya chrysanthemi bv. chrysanthemi)[J]. African Journal of Biotechnology,2008,7(4):482-486.
[5]Khalil R S. Influence of gallic acid and catechin polyphenols on probiotic properties of Streptococcus thermophilus CHCC 3534 strain[J]. World Journal of Microbiology and Biotechnology,2010,26(11):2069-2079.
[6]冯立娟,尹燕雷,招雪晴,等. 石榴没食子酸代谢与保健功能研究进展[J]. 果树学报,2014,31(4):710-716.
[7]Werner R A,Rossmann A,Schwarz C,et al. Biosynthesis of gallic acid in Rhus typhina:discrimination between alternative pathways from natural oxygen isotope abundance[J]. Phytochemistry,2004,65(20):2809-2813.
[8]Ossipov V,Salminen J P,Ossipova S,et al. Gallic acid and hydrolysable tannins are formed in birch leaves from an intermediate compound of the shikimate pathway[J]. Biochemical Systematics and Ecology,2003,31(1):3-16.
[9]Singh S A,Christendat D. The DHQ-dehydroshikimate-SDH-shikimate-NADP(H) complex:insights into metabolite transfer in the shikimate pathway[J]. Crystal Growth & Design,2007,7(11):2153-2160.
[10]Singh S A,Christendat D. Structure of arabidopsis dehydroquinate dehydratase-shikimate dehydrogenase and implications for metabolic channeling in the shikimate pathway[J]. Biochemistry,2006,45(25):7787-7796.
[11]Magalhes M B,Pereira C P,Basso L A,et al. Cloning and expression of functional shikimate dehydrogenase (EC 1.1.1.25) from Mycobacterium tuberculosis H37Rv[J]. Protein Expression and Purification,2002,26(1):59-64.
[12]Zhang X L,Zhang S B,Hao F,et al. Expression,purification and properties of shikimate dehydrogenase from Mycobacterium tuberculosis[J]. Journal of Biochemistry and Molecular Biology,2005,38(5):624-631.
[13]Kubota T,Tanaka Y,Hiraga K,et al. Characterization of shikimate dehydrogenase homologues of Corynebacterium glutamicum[J]. Applied Microbiology and Biotechnology,2013,97(18):8139-8149.
[14]Deka R K,Anton I A,Dunbar B,et al. The characterisation of the shikimate pathway enzyme dehydroquinase from Pisum sativum[J]. FEBS Letters,1994,349(3):397-402.
[15]Ding L,Hofius D,Hajirezaei M R,et al. Functional analysis of the essential bifunctional tobacco enzyme 3-dehydroquinate dehydratase/shikimate dehydrogenase in transgenic tobacco plants[J]. Journal of Experimental Botany,2007,58(8):2053-2067.
[16]Bischoff M,Schaller A,Bieri F,et al. Molecular characterization of tomato 3-dehydroquinate dehydratase-shikimate:NADP oxidoreductase[J]. Plant Physiology,2001,125(4):1891-1900.
[17]Han J W,Lee K P,Yoon M,et al. Cold stress regulation of a bi-functional 3-dehydroquinate dehydratase/shikimate dehydrogenase (DHQ/SDH)-like gene in the freshwater green alga Spirogyra varians[J]. Botanica Marina,2009,52(2):178-185.
[18]Tzin V,Galili G. New insights into the shikimate and aromatic amino acids biosynthesis pathways in plants[J]. Molecular Plant,2010,3(6):956-972.

相似文献/References:

[1]陶吉寒,招雪晴,苑兆和,等.石榴DFR基因的同源克隆及分析[J].江苏农业科学,2013,41(04):22.
[2]郭文琦,张培通,李春宏,等.沿海滩涂绿化树种选择和耐盐性评价[J].江苏农业科学,2014,42(10):175.
 Guo Wenqi,et al.Selection and evaluation on salt tolerance of gardening tree species in coastal beach areas[J].Jiangsu Agricultural Sciences,2014,42(01):175.
[3]陈成,汪洪涛.山楂石榴复合果酒发酵工艺的研究[J].江苏农业科学,2013,41(07):248.
 Chen Cheng,et al.Study on fermentation technology of hawthorn and pomegranate compound fruit wine[J].Jiangsu Agricultural Sciences,2013,41(01):248.
[4]邓志勇,吴桂容,杨程显.脐橙-石榴复合果酒酿造工艺的研究[J].江苏农业科学,2015,43(02):266.
 Deng Zhiyong,et al.Study on brewing technology of compound wine of navel orange and pomegranate[J].Jiangsu Agricultural Sciences,2015,43(01):266.
[5]范春丽,罗青.干旱胁迫下外源甜菜碱对石榴光合作用、渗透调节及保护酶活性的影响[J].江苏农业科学,2016,44(11):229.
 Fan Chunli,et al.Effects of exogenous glycinebetaine on photosynthesis,osmotic adjustment ability and protective enzyme activity of Punica granatum under drought stress[J].Jiangsu Agricultural Sciences,2016,44(01):229.
[6]鲁海菊,李河,史淑義,等.云南省石榴干腐病病菌生物学特性及其防治药剂筛选[J].江苏农业科学,2017,45(01):99.
 Lu Haiju,et al.Study on biological characteristics of pathogen of pomegranate dry rot from Yunnan Province and its control fungicides[J].Jiangsu Agricultural Sciences,2017,45(01):99.
[7]曹尚银,牛娟,张杰,等.2种蛋白质组学方法在石榴中的应用比较[J].江苏农业科学,2017,45(20):68.
 Cao Shangyin,et al.A comparative study on application of two proteomics methods in pomegranate[J].Jiangsu Agricultural Sciences,2017,45(01):68.
[8]周银丽,郭建伟,杨伟,等.间作桃树对石榴园枯萎病土壤碳代谢多样性的影响[J].江苏农业科学,2018,46(14):106.
 Zhou Yinli,et al.Influences of intercropping peach trees on fusarium wilt soil carbon metabolism diversity in pomegranate garden[J].Jiangsu Agricultural Sciences,2018,46(01):106.
[9]王硕,李德生,朱秀锦,等.石榴对镉、铅、锌复合污染土壤的修复效果[J].江苏农业科学,2019,47(02):250.
 Wang Shuo,et al.Remediation effect of pomegranate on Cd,Pb and Zn combined pollution soil[J].Jiangsu Agricultural Sciences,2019,47(01):250.
[10]方庆.不同秋水仙素处理对石榴种子发芽及幼苗生长的影响[J].江苏农业科学,2019,47(19):142.
 Fang Qing.Effects of different colchicine treatments on seed germination and seedling growth of pomegranate species[J].Jiangsu Agricultural Sciences,2019,47(01):142.

备注/Memo

备注/Memo:
收稿日期:2015-11-17
基金项目:山东省自然科学基金(编号:ZR2014YL022、ZR2015YL056);山东省果树研究所所长基金(编号:2013KY04)。
作者简介:冯立娟(1982—),女,山东聊城人,博士,助理研究员,主要从事果树遗传资源与育种研究。E-mail:flj_19820227@163.com。
通信作者:尹燕雷,硕士,副研究员,主要从事果树遗传资源与育种研究。E-mail:yylei66@sina.com。
更新日期/Last Update: 2017-01-05