[1]郄卫那,张兰英,何健,等.1株饲用木聚糖酶产生菌的分离、鉴定、发酵、酶学性质及应用[J].江苏农业科学,2014,42(10):196-200.
　Qie Weina,et al.Isolation，identification，fermentation，enzymatic properties， and application of an xylanase-producing strain for feed[J].Jiangsu Agricultural Sciences,2014,42(10):196-200.
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1株饲用木聚糖酶产生菌的分离、鉴定、发酵、酶学性质及应用()

《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第42卷
期数:
2014年10期
页码:
196-200
栏目:
畜牧兽医
出版日期:
2014-10-25

文章信息/Info

Title:
Isolation，identification，fermentation，enzymatic properties， and application of an xylanase-producing strain for feed
作者:
郄卫那1;  张兰英2;  何健1;  贺芹1
1.南京农业大学生命科学学院/农业部农业环境微生物工程重点开放实验室，江苏南京 210095；2.江西省南康市第一小学，江西南康 341400
Author(s):
Qie Weina; et al
关键词:
木聚糖酶; 酶学性质; 产酶条件; 饲料; 尖孢镰刀菌
Keywords:
-
分类号:
S182
DOI:
-
文献标志码:
A
摘要:
以木聚糖为唯一碳源，从长期堆放枯枝败叶的土壤中筛选能够高效降解木聚糖的菌株，并对该菌的产酶条件和酶学性质进行研究。分离得到1株酶活性较高且产酶相对稳定的菌株，鉴定为尖孢镰刀菌，命名为Fusarium oxysporum MJ23。研究表明，木聚糖、酵母粉是该菌的最适培养底物，在最适培养条件下，该菌株所产木聚糖酶活力可达186 U/mL；木聚糖酶的最适作用温度、pH值分别为55 ℃、6，55 ℃保温3 h，酶活力仍可维持90%以上；Ca2+、Fe2+、Mn2+、Tween 80和聚乙二醇对木聚糖酶活力有较强促进作用，Cu2+、Zn2+、EDTA、尿素和SDS则会抑制酶活，其中SDS的抑制作用最强。模拟动物体内对饲料中半纤维素组分的消化，木聚糖酶的添加可使猪饲料中木糖生成量提高28%，鸡饲料中木糖生成量提高16%，表明该菌株所产木聚糖酶具有用作饲料添加剂的可能性。
Abstract:
-

参考文献/References:

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备注/Memo

备注/Memo:
收稿日期：2013-12-07
作者简介：郄卫那(1986—)，女，河北石家庄人，硕士研究生，主要研究方向为环境微生物与环境工程。E-mail：woniubeibao@gmail.com。
通信作者：贺芹，山东枣庄人，讲师，主要研究方向为发酵工程。E-mail：qhe@njau.edu.cn。
更新日期/Last Update: 2014-10-25