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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第46卷

期数:

2018年第23期

页码:

207-211

栏目:

贮藏加工与检测分析

出版日期:

2018-12-05

文章信息/Info

Title:

Preparative purification of deoxynivalenol by combined use of macroporous resin and high-speed countercurrent chromatography

作者:

王刚;  胡俊强;  史建荣;  徐剑宏

江苏省食品质量安全重点实验室-省部共建国家重点实验室培育基地/农业农村部农产品质量安全风险评估实验室(南京)/
江苏省现代粮食流通与安全协同创新中心/江苏省农业科学院农产品质量安全与营养研究所，江苏南京 210014

Author(s):

Wang Gang; et al

关键词:

脱氧雪腐镰刀菌烯醇; 分离纯化; 大孔吸附树脂; 高速逆流色谱

Keywords:

-

分类号:

S432.4⁺4

DOI:

-

文献标志码:

A

摘要:

脱氧雪腐镰刀菌烯醇是一类危害严重的真菌毒素，针对其毒理学的研究与脱毒研究需要大量毒素纯品。采用大孔吸附树脂与高速逆流色谱联用技术，从禾谷镰刀菌PH-1的大米培养物中提取并纯化脱氧雪腐镰刀菌烯醇。首先采用85%甲醇溶液提取毒素，之后以XAD-4大孔吸附树脂柱初步纯化，用30%甲醇溶液洗脱；初步纯化产物以高速逆流色谱进行精制，采用乙酸乙酯-水(1 ∶ 1)溶剂系统进行分离，脱氧雪腐镰刀菌烯醇在150 min被洗脱，最终产物纯度为96.78%，回收率为76.20%。采用该方法可以从1 000 g大米发酵物中一次提取出500 mg以上毒素纯品。

Abstract:

-

参考文献/References:

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备注/Memo

备注/Memo:

收稿日期：2018-06-29
基金项目：江苏省农业科技自主创新资金［编号：CX(17)1003］；国家自然科学基金(编号：31471662、U1604234)；江苏省自然科学基金青年基金(编号：BK20160595)。
作者简介：王刚(1987—)，男，江苏南京人，博士，助理研究员，主要从事真菌毒素的消减方法研究。E-mail：njcpuwg@126.com。
通信作者：徐剑宏，博士，研究员，主要从事真菌毒素评估与治理研究。E-mail：xujianhongnj@126.com。

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更新日期/Last Update: 2018-12-05