[1]白雪,张慧莉,黄冲,等.解淀粉芽孢杆菌草酸脱羧酶基因的克隆、原核表达与活力测定[J].江苏农业科学,2019,47(12):66-70.
Bai Xue,et al.Cloning，prokaryotic expression and activity determination of Bacillus amyloliquefaciens oxalate decarboxylase gene[J].Jiangsu Agricultural Sciences,2019,47(12):66-70.
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解淀粉芽孢杆菌草酸脱羧酶基因的克隆、
原核表达与活力测定(PDF)
Bai Xue,et al.Cloning，prokaryotic expression and activity determination of Bacillus amyloliquefaciens oxalate decarboxylase gene[J].Jiangsu Agricultural Sciences,2019,47(12):66-70.
原核表达与活力测定(PDF)
《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]
- 卷:
- 第47卷
- 期数:
- 2019年第12期
- 页码:
- 66-70
- 栏目:
- 生物技术
- 出版日期:
- 2019-07-10
文章信息/Info
- Title:
- Cloning，prokaryotic expression and activity determination of Bacillus amyloliquefaciens oxalate decarboxylase gene
- Keywords:
- -
- 分类号:
- Q786；S188
- DOI:
- -
- 文献标志码:
- A
- 摘要:
- 为进一步明确解淀粉芽孢杆菌C(10)的草酸脱羧酶基因的生物功能，克隆其关键基因对该基因进行原核表达。通过PCR技术克隆了淀粉芽孢杆菌C(10)的草酸脱羧酶基因，构建了原核生物表达载体pET28a-Baoxdc，对其进行表达与纯化。结果表明，扩增所获解淀粉芽孢杆菌C(10)的草酸脱羧酶全基因序列全长为1 161 bp，在大肠杆菌中进行表达，添加诱导剂IPTG、MnCl2至终浓度分别为0.6、6 mmol/L，诱导4 h时的酶活力和比活力最高，分别为3793 2 U/mL和3.145 3 U/mg。
- Abstract:
- -
参考文献/References:
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备注/Memo
- 备注/Memo:
-
收稿日期：2018-02-27
基金项目：国家自然科学基金(编号：21366028)。
作者简介：白雪(1992—)，女，甘肃人，硕士研究生，主要从事微生物学发酵工程方向研究。E-mail：937094198@qq.com。
通信作者：张慧莉，博士，讲师，主要从事分子生物学及生物工程方向研究。E-mail：zhanghch02@126.com。
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