|本期目录/Table of Contents|

[1]王春霞,王晶,曹子健,等.玉米根毛单细胞类型转录组分析[J].江苏农业科学,2022,50(3):49-58.
　Wang Chunxia,et al.Transcriptome analysis of single cell type of root hair in maize[J].Jiangsu Agricultural Sciences,2022,50(3):49-58.
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玉米根毛单细胞类型转录组分析(PDF)
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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:
第50卷
期数:
2022年第3期
页码:
49-58
栏目:
“转录组”专题
出版日期:
2022-02-05

文章信息/Info

Title:
Transcriptome analysis of single cell type of root hair in maize
作者:
王春霞1; 2;  王晶1;  曹子健1;  胡宝1;  许子洁1;  陈立群2
1.河北工程大学园林与生态工程学院，河北邯郸 056038；2.中国农业大学生物学院植物生理学与生物化学国家重点实验室，北京 100193
Author(s):
Wang Chunxia; et al
关键词:
玉米; 根毛; 单子叶植物; 转录组分析; 特异表达基因
Keywords:
-
分类号:
S513.01
DOI:
-
文献标志码:
A
摘要:
根毛是植物根表皮细胞向外突起伸长形成的管状单细胞，在植物水分、营养物质吸收及与外界环境相互作用的过程中发挥重要功能。目前，对玉米根毛生长发育的研究极其有限，玉米作为一种单子叶植物，根毛生长发育的分子机制与十字花科植物拟南芥可能有所不同。为探究玉米根毛生长发育的调控机制，以玉米自交系B73根毛为研究对象，收集生长3 d的根毛进行单细胞类型的转录组测序分析。结果表明，各组样品高质量碱基均达到6 Gb以上，各组样品的数据比对率均在85%以上。筛选到1 050个根毛特异表达基因，其中含有68个转录因子。GO富集分析发现，玉米根毛特异表达基因主要集中在细胞壁合成、离子结合、氧化还原反应及应答逆境胁迫过程。将玉米根毛特异表达基因进行同源比对，鉴定到23个同源基因。分析转录组数据还发现玉米根毛细胞中存在内含子保留这一特异可变剪切形式。qRT-PCR 验证结果表明，9个根毛特异表达基因的表达与转录组分析的结果相符合，证明了转录组数据的可靠性。研究结果解析了玉米根毛生长发育的转录调控分子机制，为后续研究提供了重要基因数据信息。
Abstract:
-

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备注/Memo

备注/Memo:
收稿日期：2021-07-07
基金项目：河北省现代农业产业技术体系食用菌创新团队建设专项(编号：HBCT2018050202)；河北省重点研发计划项目现代种业科技专项(编号：19222903D)；河北省高等学校学科技术研究项目(编号：QN2021210)。
作者简介：王春霞(1989—)，女，河北石家庄人，博士，主要从事玉米根毛生长发育机制研究。E-mail：wangchunxia@hebeu.edu.cn。
通信作者：陈立群，博士，副教授，主要从事植物发育生物学研究。E-mail：15933560026@163.com。
更新日期/Last Update: 2022-02-05