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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第48卷

期数:

2020年第03期

页码:

80-85

栏目:

专论与综述

出版日期:

2020-03-15

文章信息/Info

Title:

Research progress on application of loop-mediated isothermal amplification(LAMP) for detection of salmonella in food

作者:

魏桢元

腾德商品检测(上海)有限公司，上海201114

Author(s):

Wei Zhenyuan

关键词:

环介导等温扩增技术; 沙门氏菌; 食源性致病菌; 现场检测; 研究进展

Keywords:

-

分类号:

TS207.4

DOI:

-

文献标志码:

A

摘要:

沙门氏菌是世界上最常见的食源性致病菌之一，全球每年沙门氏菌中毒事件居细菌性中毒事件首位。环介导等温扩增技术(loop-mediated isothermal amplification，LAMP)是一种新型的核酸恒温扩增方法。2005年首次报道了采用LAMP技术快速检测沙门氏菌的研究，在随后的十几年中，LAMP技术不断优化完善，并广泛应用于食品沙门氏菌的现场检测。本综述阐述LAMP的技术原理和特点，归纳基于LAMP技术的不同平台在沙门氏菌快速检测中的应用，同时对LAMP技术假阳性率高的问题进行了探讨，并对LAMP技术的发展方向和研究趋势提出了几个设想。

Abstract:

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备注/Memo

备注/Memo:

收稿日期：2019-08-27
作者简介：魏桢元(1984—)，男，硕士，工程师，从事食品安全检测。E-mail：wzyits@163.com。

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更新日期/Last Update: 2020-02-05