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《江苏农业科学》[ISSN:1002-1302/CN:32-1214/S]

卷:

第51卷

期数:

2023年第11期

页码:

1-10

栏目:

专论与综述

出版日期:

2023-06-05

文章信息/Info

Title:

Research progress of CRISPR/Cas gene editing system in rice

作者:

刘建菊; 肖宁; 吴云雨; 蔡跃; 潘存红; 时薇; 陈梓春; 朱书豪; 李育红; 余玲; 王志平; 刘广青; 周长海; 黄年生; 张小祥; 季红娟; 李爱宏

1.江苏里下河地区农业科学研究所/国家水稻产业技术体系扬州试验综合站，江苏扬州 225007；2.扬州大学农学院/植物功能基因组学教育部重点实验室/江苏省作物基因组学和分子育种重点实验室，江苏扬州 225009；3.江苏省现代作物生产协同创新中心，江苏南京 210095

Author(s):

Liu Jianju; et al

关键词:

基因编辑; Cas9; Cas12a; 水稻; 性状改良

Keywords:

-

分类号:

S511.01

DOI:

-

文献标志码:

A

摘要:

基因编辑是一种能对特定基因进行修饰的基因工程技术，能快速对靶点基因编辑，是高效捕获目的基因、快速研究目标基因功能的重要手段，在基因功能研究和作物育种等方面有着重要意义和广阔的应用前景。基因编辑利用特异的DNA结合元件和切割元件开展编辑工作，然而该技术最需注意的是特异性和脱靶率问题，不同时期的基因编辑技术也针对上述2个问题进行改良，目前应用最为广泛的是CRISPR/Cas9，Cas12a 由于其特异性高且脱靶率大大降低也受到越来越多的关注。本文对基因编辑的技术发展及特点、CRISPR/Cas9和Cas12a的技术优势进行介绍，并对这2种技术在水稻产量、抗性及品质中的研究进展进行综述，同时对拓展CRISPR/Cas基因编辑技术在水稻中的应用提出展望，为基因功能鉴定及遗传改良提供参考。

Abstract:

-

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备注/Memo

备注/Memo:

收稿日期：2023-05-17
基金项目：江苏省农业科技自主创新资金［编号：CX(21) 3101］；江苏省自然科学基金(编号：BK20220307)；国家现代农业产业技术体系建设专项(编号：CARS-01-65)。
作者简介：刘建菊(1989—)，女，河北保定人，博士，助理研究员，主要从事水稻遗传育种与分子生物学研究。E-mail：jianjuliu23@163.com。
通信作者：李爱宏，博士，研究员，主要从事水稻遗传育种研究。E-mail：yzlah@126.com。

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更新日期/Last Update: 2023-06-05